Abstract
Introduction
The present study investigates the effect of oral consumption of hydrogen sulfide-containing Harkány thermal spring water, as well as sodium hydrogen sulfide (NaHS) solution on experimental colitis.
Methods
Colitis was induced by 2 % dextran sulfate sodium (DSS) in the drinking water of C57BL/6 mice for 7 days. Some animal groups drank Harkány thermal spring water or water supplemented with 21.68 mg/L NaHS. General signs of colitis, myeloperoxidase (MPO) enzyme activity of colon samples, histological features of colitis and function of the enteric nervous system were assessed.
Results
Oral administration of Harkány thermal spring water significantly attenuated general signs of colitis, MPO enzyme activity of colon samples and detrimental effect of colitis on the function of the enteric nervous system, but not histological signs of colitis. These findings could be reproduced using NaHS solution with additional significantly diminished histological damage.
Conclusions
We conclude that oral treatment with Harkány thermal spring water relieves various aspects of DSS-evoked colitis in mice. This effect is most likely to be mediated by hydrogen sulfide content of the Harkány water. Our data might promote complementary utilization of sulfurous thermal spring water in the therapy of inflammatory bowel disease.
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Acknowledgments
The study was supported by Baross Gábor Program and SROP-4.2.2.A-11/1/KONV-2012-0024. E. P. was funded by Szentágothai János Fellowship A2-SZJÖ-TOK-13-0149.
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The authors declare that they have no conflict of interest.
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Pozsgai, G., Benkó, R., Barthó, L. et al. Thermal spring water drinking attenuates dextran-sulfate-sodium-induced colitis in mice. Inflammopharmacol 23, 57–64 (2015). https://doi.org/10.1007/s10787-014-0227-7
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DOI: https://doi.org/10.1007/s10787-014-0227-7