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CircANKRD36 Knockdown Suppressed Cell Viability and Migration of LPS-Stimulated RAW264.7 Cells by Sponging MiR-330

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Abstract

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is an independent risk factor for mortality in patients with sepsis. In this study, we attempt to investigate the molecular mechanism of circANKRD36 underlying sepsis-induced ALI/ARDS in vitro. We first detected the altered circRNAs in serums of patients with sepsis-induced ARDS using circRNAs microarray. CircANKRD36 expression in serums and LPS-stimulated RAW264.7 cells was measured using qRT-PCR. CCK-8, cell migration, ELISA, and qRT-PCR were applied to the evaluation of cell biological behavior and inflammation reaction. The results showed that circANKRD36 expression was significantly elevated in serum of patients with sepsis-induced ARDS. Knockdown of circANKRD36 inhibited cell viability and migration and alleviated inflammation of lipopolysaccharide-stimulated (LPS-stimulated) RAW264.7 cells. Bioinformatic analysis demonstrated that circANKRD36 serves as a sponge for miR-330 and ROCK1 was directly targeted by miR-330. Furthermore, knockdown of circANKRD36 repressed ROCK1 expression by targeting miR-330. In short, circANKRD36 knockdown suppressed cell viability and migration of LPS-stimulated RAW264.7 cells in vitro via sponging miR-330, which may provide new ideas for the treatment of sepsis-induced ARDS.

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Availability of Data and Materials

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Emergency Medicine, The Affiliated Hospital of Youjiang Medical University for Nationalities, No. 18, Zhongshan Second Road, Yooujiang District, Baise City, 533000, China

    Qiqing Lin

  2. Respiratory Department, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, 533000, China

    Qiong Liang & Chunyan Qin

  3. Department of Interventional Medicine, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, 533000, China

    Yueyong Li

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Contributions

Qiqing Lin and Qiong Liang designed experiments; Chunyan Qin and Yueyong Li performed experiments; Qiong Liang analyzed the data; Qiqing Lin wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Qiqing Lin.

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All patients gave informed consent and this study was approved by the ethics committee of The Affiliated Hospital of Youjiang Medical University for Nationalities.

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Lin, Q., Liang, Q., Qin, C. et al. CircANKRD36 Knockdown Suppressed Cell Viability and Migration of LPS-Stimulated RAW264.7 Cells by Sponging MiR-330. Inflammation 44, 2044–2053 (2021). https://doi.org/10.1007/s10753-021-01480-5

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