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Glycitin Suppresses Cartilage Destruction of Osteoarthritis in Mice

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Abstract

Osteoarthritis (OA), a chronic joint disease, is characterized by cartilage surface erosion, subchondral bone rebuilding, and formation of osteophytes. To date, the nosogenesis and underlying mechanisms of OA have not yet been elucidated. However, it is widely accepted that TNF-α is a crucial cytokine in the development of OA. Glycitin, a natural isoflavone extracted from legumes, affects physiological reactions and pathological responses. Recently, the anti-inflammatory effect of glycitin has been reported. However, the function of glycitin in cartilage degeneration in OA remains to be investigated. In the current study, primary murine chondrocytes were isolated and stimulated by TNF-α to evaluate the anti-inflammatory effects and protective function of glycitin in chondrocytes. In vivo, the ACLT mouse model, a frequently-used OA model, was used to further examine the therapeutic role of glycitin in cartilage degeneration and inflammation in OA. Consequently, glycitin functions were examined both in vivo and in vitro. Moreover, the underlying mechanism of action of glycitin was investigated and was found to involve the NF-κB signaling pathway. Collectively, this study suggests that glycitin can be potentially used for the treatment of joint degenerative diseases, including OA.

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Funding

This work was supported by the Natural Science Foundation of Shandong Province (Grant Nos. ZR2019MH05, ZR2019MH075, and BS2015SW028), the Key Research and Development Projects of Shandong Province (Nos. 2015GSF118115 and 2019GSF108152), the Cross-disciplinary Fund of Shandong University (Grant No. 2018JC007), and the National Natural Science Foundation of China (Grant Nos. 81501880 and 81602761).

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Authors and Affiliations

  1. Department of Orthopedics, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People’s Republic of China

    Wenhan Wang, Ruitong Yang, Yunpeng Zhao, Hao Li & Xin Pan

  2. Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, People’s Republic of China

    Wenhan Wang, Ruitong Yang, Jiayi Li, Jiangfan Peng & Mingyang Xu

  3. Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Jinan, Shandong, 250012, People’s Republic of China

    Minfa Zhang

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  1. Wenhan Wang
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  9. Xin Pan
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Contributions

Conceived and designed the experiments: Hao Li, Xin Pan.

Performed the experiments: Wenhan Wang, Ruitong Yang, Minfa Zhang, Jiayi Li, Jiangfan Peng, Mingyang Xu, Yunpeng Zhao.

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Correspondence to Hao Li or Xin Pan.

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Wang, W., Yang, R., Zhang, M. et al. Glycitin Suppresses Cartilage Destruction of Osteoarthritis in Mice. Inflammation 43, 1312–1322 (2020). https://doi.org/10.1007/s10753-020-01210-3

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