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Protective Effect of Gentianine, a compound from Du Huo Ji Sheng Tang, against Freund’s Complete Adjuvant-Induced Arthritis in Rats

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Abstract

The anti-inflammatory property of gentianine (GE) aroused our interest. To investigate the effect of GE on Freund’s complete adjuvant (FCA)-induced arthritis in rats was the aim of this study. Adjuvant-induced arthritis (AA) was induced by the administration of 0.1 ml FCA in the surface of hind paw. Diclofenac sodium (DS) and Fasudil were administered as positive controls. Downregulation of cytokines including interleukin-6 (IL-6), interleukin-1 beta (IL-1β), and tumor necrosis factor-alpha (TNF-α) revealed anti-inflammatory effect of GE, which was consisted with pathological conditions of rat paws. In addition, western blot analysis revealed that GE could strikingly suppressed the expressions of Rho/NF-κB signaling pathways as well as TGF-β/smad-3 pathways. In conclusion, data indicated that GE might contribute to treat arthritis as a potential therapeutic candidate.

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Authors and Affiliations

  1. Nanjing University of Chinese Medicine, Nanjing, China

    Chen Wenjin

  2. Wuxi Hospital affiliated to Nanjing University of Chinese Medicine, Wuxi, China

    Wang Jianwei

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  1. Chen Wenjin
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  2. Wang Jianwei
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Correspondence to Wang Jianwei.

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All animal experiments were undertaken in accordance with the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals.

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The authors declare that they have no conflicts of interest.

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Wenjin, C., Jianwei, W. Protective Effect of Gentianine, a compound from Du Huo Ji Sheng Tang, against Freund’s Complete Adjuvant-Induced Arthritis in Rats. Inflammation 40, 1401–1408 (2017). https://doi.org/10.1007/s10753-017-0583-8

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  • DOI: https://doi.org/10.1007/s10753-017-0583-8

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