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Activation of Neutrophils via IP3 Pathway Following Exposure to Demodex-Associated Bacterial Proteins

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Abstract

Rosacea is a chronic inflammatory condition that predominantly affects the skin of the face. Sera from rosacea patients display elevated reactivity to proteins from a bacterium (Bacillus oleronius) originally isolated from a Demodex mite from a rosacea patient suggesting a possible role for bacteria in the induction and persistence of this condition. This work investigated the ability of B. oleronius proteins to activate neutrophils and demonstrated activation via the IP3 pathway. Activated neutrophils displayed increased levels of IP1 production, F-actin formation, chemotaxis, and production of the pro-inflammatory cytokines IL-1β and IL-6 following stimulation by pure and crude B. oleronius protein preparations (2 μg/ml), respectively. In addition, neutrophils exposed to pure and crude B. oleronius proteins (2 μg/ml) demonstrated increased release of internally stored calcium (Ca2+), a hallmark of the IP3 pathway of neutrophil activation. Neutrophils play a significant role in the inflammation associated with rosacea, and this work demonstrates how B. oleronius proteins can induce neutrophil recruitment and activation.

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Acknowledgments

F. McM is funded by the Programme in BioAnalysis and Therapeutics (BioAT) by the Higher Education Authority of Ireland through PRTLI V.

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Authors and Affiliations

  1. Department of Biology, Maynooth University, Co. Kildare, Ireland

    Fred McMahon & Kevin Kavanagh

  2. Respiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin 9, Ireland

    Nessa Banville, David A. Bergin & Emer Reeves

  3. Mabtech AB, Nacka Strand, Sweden

    Christian Smedman & Staffan Paulie

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  1. Fred McMahon
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  2. Nessa Banville
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  3. David A. Bergin
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  7. Kevin Kavanagh
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Correspondence to Kevin Kavanagh.

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S. Paulie is employed by, and part-owner of, Mabtech AB. C. Smedman is employed by Mabtech AB. The other authors (F.McMahon, N. Banville, D. Bergin, E. Reeves, K. Kavanagh) have no conflicts of interest to declare.

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McMahon, F., Banville, N., Bergin, D.A. et al. Activation of Neutrophils via IP3 Pathway Following Exposure to Demodex-Associated Bacterial Proteins. Inflammation 39, 425–433 (2016). https://doi.org/10.1007/s10753-015-0264-4

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