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Anti-Asthmatic Effects of Ginsenoside Rb1 in a Mouse Model of Allergic Asthma Through Relegating Th1/Th2

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Abstract

The aim of the study was to investigate the anti-asthma effects of ginsenoside Rb1 (Rb1) and its possible mechanisms. A total of 50 mice were randomly assigned to five experimental groups: control, model, dexamethasone (2 mg/kg), and Rb1 (10 and 20 mg/kg). Airway resistance (RI) was measured; histological studies were evaluated by the hematoxylin and eosin (HE) staining; Th1/Th2, ovalbumin (OVA)-specific serum, and bronchoalveolar lavage fluid (BALF) IgE levels were evaluated enzyme-linked immunosorbent assay (ELISA); and T-bet/GATA3 proteins were evaluated by Western blot. Our study demonstrated that Rb1 inhibited OVA-induced increases in RI and eosinophil counts; interleukin (IL)-4 was recovered, and IFN-γlevel increased in bronchoalveolar lavage fluid. Histological studies demonstrated that Rb1 substantially inhibited OVA-induced eosinophilia in lung tissue. Western blot studies demonstrated that Rb1 substantially inhibited GATA3 and increased T-bet. These findings suggest that Rb1 may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.

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Acknowledgments

This work was supported by a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjiaxiang, Nanjing, 210009, Jiangsu Province, People’s Republic of China

    Tong Chen & Hui Ji

  2. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, 210009, China

    Shiping Ma

  3. Department of Physiology and Pharmacology, China Pharmaceutical University, Nanjing, China

    Lu Xiao, Lingpeng Zhu & Tianhua Yan

Authors
  1. Tong Chen
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  2. Lu Xiao
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  3. Lingpeng Zhu
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  4. Shiping Ma
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  5. Tianhua Yan
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Correspondence to Hui Ji.

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Chen, T., Xiao, L., Zhu, L. et al. Anti-Asthmatic Effects of Ginsenoside Rb1 in a Mouse Model of Allergic Asthma Through Relegating Th1/Th2. Inflammation 38, 1814–1822 (2015). https://doi.org/10.1007/s10753-015-0159-4

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  • DOI: https://doi.org/10.1007/s10753-015-0159-4

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