Abstract
Prostate cancer is one of the most common neoplasm in the male population. It is not known why some tumors become more aggressive than others. Although most studies show changes in the expression of cell adhesion molecules and the extracellular matrix correlated with the Gleason score, no study has objectively measured the tissue content of these molecules. This study aims to measure the content and tissue expression of collagen type I and IV and laminin in the extracellular matrix of patients with prostate adenocarcinoma and correlate these findings with the Gleason score and clinical characteristics. Forty-one patients who underwent radical prostate surgery at the Urology Department of a reference Hospital in Brazil between January 2015 and December 2020 were studied. The tissue protein content was estimated under light microscopy at a final magnification of 200 × . The mean collagen I score in prostate adenocarcinoma tissue samples was 7.16 ± 1.03 pixels/field. The mean type IV collagen score was 3.44 ± 0.61 pixels/field. The mean laminin score was 5.19 ± 0.79 pixels/field. The total Gleason score was correlated with both collagen and laminin. All the correlations were negative, which shows that the higher the collagen/laminin expression was, the lower the total Gleason score (p-value < 0,05). According to the Pearson correlation analysis, age has no statistical relationship with collagen and laminin content. PSA, in turn, showed a correlation only with laminin, but r = -0.378 (p = 0.015). Among the associated diseases and lifestyle habits, there is only statistical significance in the comparison of alcoholism for collagen I. For collagen IV and laminin, no statistical significance was obtained with the clinical variables analyzed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data availability
No datasets were generated or analysed during the current study.
Abbreviations
- ANOVA:
-
Analysis of variance
- EAEC:
-
Ethics Applied Evaluation Committee
- ECM:
-
Extracellular matrix
- HUSF:
-
São Francisco de Assis University Hospital
- Pca:
-
Prostate cancer
- PSA:
-
Prostate-specific antigen
References
Brum IS, Spritzer PM, Brentani MM (2005) Biologia molecular das neoplasias de próstata. Arq Bras Endocrinol Metab 49(5):797–804
Burns-Cox N, Avery NC, Gingell CN, Bailey AJ (2001) Changes In collagen metabolism in prostate cancer: a host response that may alter progression. J Urol 166(5):1698–1701
Cambruzzi E, Zettler CG, Pegas KL, Teixeira SL (2010) Relação entre escore de gleason e fatores prognósticos no adenocarcinoma acinar de próstata. J Bras Patol Med Lab 46(1):61–68
Cavallaro U, Christofori G (2004) Multitasking in tumor progression: signaling functions of cell adhesion molecules. Ann N Y Acad Sci 1014(1):58–66
Chou R, Griffin J, Cheney T (2022) Management of clinically localized prostate cancer: a systematic evidence review. Pac NW Evid Based Pract 23(2):150–162
Duarte AH, Colli S, Alves-Pereira JL, Martins MP, Sampaio Francisco JB, Ramos CF (2012) Collagen I and III and metalloproteinase gene and protein expression in prostate cancer in relation to Gleason score. Int Braz J Urol 38(3):341–354
Dubik N, Mai S (2020) Lamin A/C: Function in normal and tumor cells. Cancers 12(12):3688
Instituto Nacional do Câncer (2023) Câncer De Próstata. Disponível em. https://www.inca.gov.br/tipos-de-cancer/cancer-de-prostata. Acesso em: 1 de maio de
Kordan Y, Chang SS, Salem S, Cokson MS, Clark PE, Davis R, Herrell SD, Baumgartner R, Phillips S, Smith Jr JA, Barocas DA (2009) Pathological stage T2 subgroups to predict biochemical recurrence after prostatectomy. J Urol 182(5):2291–2295
Korkes F, Smaidi K, Timoteo F, Glina S (2022) Recommendations for prostate cancer diagnosis and treatment during COVID-19 outbreak were not followed in Brazil. Int Braz J Urol 48(4):712–718
Luthold C, Hallal T, Labbé DP, Bordeleau F (2022) The extracellular matrix stiffening: a trigger of prostate cancer progression and castration resistance?. Cancers, 14(12):2887. https://doi.org/10.3390/cancers14122887
Ma J, Bai J, Zhang H, Gu L, He D, Guo P (2020) Downregulation of collagen COL4A6 is associated with prostate cancer progression and metastasis. Genet Test Mol Biomarkers 24(7):399–408
Norris JM, Carmona LM, Bott-Simon RJ, Brown LC, Burns-Cox N, Dudderidge T, Bosaily AE, Frangou E, Freeman A, Ghei M, Henderson A, Hindley RG, Kaplan RS, Kirkham A, Oldroyd R, Parker C, Persad R, Punwani S, Rosario DJ, Shergill IS, Stavrindes V, Winkler M, Whitaker HC, Ahmed HU, Emberton M (2020) What type of prostate cancer is systematically overlooked by multiparametric magnetic resonance imaging? An analysis from the PROMIS Cohort. Eur Urol 78(2):163–170
Podgorski I, Linebaugh BE, Sameni M, Jedeszko C, Bhagat S, Cher ML, Sloane BF (2005) Bone Microenvironment Modulates Expression and Activity of Cathepsin B in prostate cancer. Neoplasia 7(3):207–223
Rasmussen AE, Douglas GK, Karsdal Morten A (2016) Biochemistry Of Collagens Laminins And Elastin. Academic Press 15(3):163–196
Sehn JK (2018) Prostate cancer pathology: recent updates and controversies. Mo Med 115(2):151–155
Shimojo H, Kobayashi M, Kamigaito T, Shimojo Y, Fukuda M, Nakayama J (2011) Reduced Glycosylation of α-dystroglycans on carcinoma cells contributes to formation of highly infiltrative histological patterns in prostate cancer: reduced glycosylation of α-dystroglycans. Prostate 71(11):1151–1157
Sociedade Brasileira de Urologia (2023) Câncer de Próstata. Disponível em. https://sbu-sp.org.br/publico/cancer-de-prostata-3/. Acesso em: 3 de maio de
Velosa APP, Teodoro WR, Yoshinari NH (2003) Colágeno na cartilagem osteoartrótica. Rev Bras Reumatol 43(3):160–166
Acknowledgements
The project was approved by the Ethics Committee of the University of São Francisco, located in Bragança Paulista, State of São Paulo. The approval number was 4,823,523
Author information
Authors and Affiliations
Contributions
Igor Arantes is the main author, he was present at all stages of the project. Maria Roberta, Enrico, Mateus and Geovanna assisted in the elaboration of the project as well as the reading of the slides. José Aires coordinated the reading of the slides. Marcos Castro and Carlos Martinez assisted in the writing of the article and its revision.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Novelty and impact
No study has objectively measured the content and tissue expression of collagen type I and IV and laminin in the extracellular matrix of patients with prostate adenocarcinoma and correlate these findings with the Gleason score and clinical characteristics. This results can be useful and lead us to believe it can become a relevant marker of worse prognosis in Prostate Cancer patients
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Góes, I.A., Pereira, M.R.M., Crotti, E. et al. Content and tissue expression of Collagen I, Collagen IV, and Laminin in the Extracellular Matrix in Prostate Adenocarcinoma. J Mol Histol 55, 371–378 (2024). https://doi.org/10.1007/s10735-024-10196-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10735-024-10196-3