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Arabidopsis SSL1 encoding AtMWFE subunit of mitochondrial complex I regulates leaf development and reactive oxygen species

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Abstract

Mitochondrial complex I is an NADH-ubiquinone oxidoreductase responsible for 40% of the production of mitochondrial ATP. It contains 14 core subunits and 25–35 non-core ones in different organisms. However, the role of these subunits in plant development remains largely unknown. Here, we report a novel Arabidopsis T-DNA insertion mutant. The T-DNA insertion mutant produced smaller and more serrated leaves than wild-type control. So, it is named that the Arabidopsis small and serrated leaves 1 (ssl1). We identified a T-DNA insertion in the AtMWFE locus- that disrupted the function of AtMWFE in ssl1. AtMWFE encodes a conserved non-core subunit of mitochondrial complex I. The expression of AtMWFE complemented the leaf developmental defects of ssl1- thus SSL1 is the AtMWFE subunit of mitochondrial complex I. We also showed that the compromise of SSL1/AtMWFE function led to the accumulation of ROS. Our findings reveal that SSL1/AtMWFE is required for the function of mitochondrial complex I and the proper ROS level in leaves, and demonstrate that SSL1/AtMWFE plays a critical role in leaf development.

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Acknowledgements

This research was supported by the Applied Basic Research Project of Datong of China (Grant No. 2022053). We would like to thank Prof. Qin Genji (Peking University) for presenting the ssl1 mutant as a gift.

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Authors and Affiliations

  1. College of Agricultural and Life Science, Shanxi Datong University, Datong, 037009, China

    Liu Jianxia, Zhang Yongfang, Xue Naiwen, Cao Huifen & Wu Juan

  2. Research and Development Center of Agricultural Facility Technology, Shanxi Datong University, Datong, 037009, China

    Liu Jianxia, Zhang Yongfang & Xue Naiwen

  3. Maize Research Institute, Shanxi Agricultural University, Xinzhou, 034000, China

    Wen Riyu

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  1. Liu Jianxia
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Contributions

J.L. and Y. Z. performed the experiments. F. C. and J. W. designed the experiments, performed the experiments, analyzed the data and wrote the manuscript. N.X. and R.W. conceived the project, designed the experiments, analyzed the data and wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Wen Riyu.

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Jianxia, L., Yongfang, Z., Naiwen, X. et al. Arabidopsis SSL1 encoding AtMWFE subunit of mitochondrial complex I regulates leaf development and reactive oxygen species. Plant Growth Regul (2024). https://doi.org/10.1007/s10725-024-01135-z

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