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Genome-wide analysis of HAK/KUP/KT potassium transporter genes in banana (Musa acuminata L.) and their tissue-specific expression profiles under potassium stress

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Abstract

Potassium is one of the most essential inorganic cations for plant growth and development. The high affinity K+ (HAK)/K+ uptake (KUP)/K+ transporter (KT) family plays essential roles in the regulation of cellular K+ levels and the maintenance of osmotic balance. However, the roles of these genes in the responses of bananas to low-potassium stress are unclear. In this study, 24 HAK/KUP/KT (MaHAK) genes were identified from banana. These genes were further classified into four groups based on phylogenetic analysis, gene structure and conserved domain analysis. Segmental duplication events played an important role in the expansion of the MaHAK gene family. Transcriptome analysis revealed the expression patterns of MaHAKs in various tissues under different K+ conditions. MaHAK14b was upregulated under both short- and long-term K+-deficient conditions, suggesting that it plays crucial roles in K+ uptake at low K+ concentrations. Furthermore, MaHAK14b mediated K+ uptake when it was heterologously expressed in the yeast mutant R5421 on low K+ medium. Collectively, these findings provide a foundation for further functional analysis of MaHAK genes, which may be used to improve potassium stress resistance in bananas.

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Funding

This research was financially supported by the Guangdong Basic and Applied Basic Research Foundation (2020A1515110731), Guangdong Science and technology planning project (2021A0505030049), the Director Fund of the Institute of Fruit Research, Guangdong Academy of Agricultural Sciences, the Special Fund for Scientific Innovation Strategy–Construction of High Level Academy of Agriculture Science (R2018QD-021), the Special Fund Project for Talent Introduction of Guangdong Academy of Agriculture Science.

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Author notes

  1. Siwen Liu and Bangting Wu have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Key Laboratory of Tropical and Subtropical Fruit Tree Research of Guangdong Province, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Siwen Liu, Bangting Wu, Yanling Xie, Dandan Xiang & Chunyu Li

  2. Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650224, China

    Sijun Zheng

  3. Bioversity International, Kunming, 650224, China

    Sijun Zheng

  4. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Jianghui Xie & Wei Wang

Authors
  1. Siwen Liu
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  2. Bangting Wu
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  3. Yanling Xie
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  4. Sijun Zheng
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  5. Jianghui Xie
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  6. Wei Wang
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Correspondence to Chunyu Li.

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Communicated by Luca Sebastiani .

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Liu, S., Wu, B., Xie, Y. et al. Genome-wide analysis of HAK/KUP/KT potassium transporter genes in banana (Musa acuminata L.) and their tissue-specific expression profiles under potassium stress. Plant Growth Regul 97, 51–60 (2022). https://doi.org/10.1007/s10725-021-00793-7

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