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Pollen germination, pollen–pistil interaction and crossability studies in interspecific and induced colchiploid population of Abelmoschus species

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Abstract

Okra is one of the important vegetable crops suffering from yield losses due to various biotic stresses. Crop wild relatives are reservoir of genetic resistance to biotic stresses but their utilization in crop breeding is restricted by various pre- and post-zygotic crossing barriers. In our study, multiple stress tolerant lines ‘IC90476-1’ of Abelmoschus manihot var. tetraphyllus and (IC141055) of Abelmoschus moschatus were crossed reciprocally with commercially grown variety Pusa A-4 of A. esculentus. Subsequently, only direct cross (Abelmoschus esculentus as female parent) F1s, amphidiploidized (C1), selfed and backcrossed with A. esculentus variety Pusa A-4 were raised to investigate the crossability barriers. Abelmoschus manihot var. tetraphyllus was found to be bi-directionally crossable with A. esculentus and not hampered by any serious pre- and post-zygotic barriers. But differences in ploidy leads to failing in production of F1 seeds upon selfing and backcrossing. Therefore, amphidiploidization of F1 hybrid followed by selfing and backcrossing (amphidiploid as seed parent) is recommended as an effective strategy to transfer desirable traits from A. manihot var. tetraphyllus to A. esculentus. However, A. moschatus × A. esculentus crosses suffered from severe pre-zygotic barriers that resulted in formation of aborted embryo with partially filled seeds. Therefore, use of A. esculentus only as female parent, in crossing with A. moschatus may increase the production of viable F1 seeds. In conclusion, colchicine (0.1%) induced amphidiploidization was found effective for restoration of fertility. However greater success was obtained in reciprocal backcrossing (A. esculentus × F1/C1) compared to amphidiploidization followed by selfing of C1 was recommended for gene introgression from A. moschatus.

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Abbreviations

YVMV :

Yellow vein mosaic virus

ELCV:

Enation leaf curl virus

AES :

A. esculentus

AMT :

A. manihot var. tetraphyllus

AM :

A. moschatus

ABF :

Aniline blue fluorescence

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Funding

There is no specific financial assistance provided for this work. Only institute funding by ICAR-Indian Agricultural Research Institute, New Delhi.

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Authors and Affiliations

  1. Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 11002, India

    Mahesh Badiger, Ramesh Kumar Yadav, Brij Bihari Sharma, Bhoopal Singh Tomar, Suman Lata, N. D. Vinay & Anjan Das

  2. ICAR-National Bureau of Plant Genetic Resources, New Delhi, 11002, India

    K. V. Bhat

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  1. Mahesh Badiger
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  2. Ramesh Kumar Yadav
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  4. K. V. Bhat
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  7. N. D. Vinay
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Contributions

MB: Investigation, analysis, Writing—Original Draft; RKY: conceptualization, execuition, Resources, Supervision, Review & Editing; BBS: analysis, Editing; KVB: Editing, BST: Editing; SL: editing, Analysis; VNB: Data curation; AD: Analysis.

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Correspondence to Ramesh Kumar Yadav.

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Badiger, M., Yadav, R.K., Sharma, B.B. et al. Pollen germination, pollen–pistil interaction and crossability studies in interspecific and induced colchiploid population of Abelmoschus species. Genet Resour Crop Evol 71, 107–127 (2024). https://doi.org/10.1007/s10722-023-01610-y

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