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Waterlogging tolerance evaluation methods for soybean (Glycine max (L.) Merr.) at the pregermination stage

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Abstract

Soybean is an important oil- and protein-rich crop worldwide. It is vital to identify soybean genotypes that show tolerance at initial crop growth stages (germination to fourth tri-foliate leaf stage) that coincide with a heavy monsoon. A set of 964 diverse soybean accessions was evaluated for pregermination anaerobic stress (pGAS) tolerance for different durations (3, 5, 7 and 9 days) under the paper towel method in artificial conditions. After nine days of submergence, the EC accessions exhibited significantly higher percent germination (11%), followed by IC germplasm (8.11%), breeding lines (2.40%) and the commercial varieties (2.12%). Ninety-seven genotypes showing phenotypic variation during the initial screening were further evaluated for waterlogging tolerance in perforated cups, and germination percentage was recorded after nine days of stress treatment. Forty-five of these 97 genotypes surviving waterlogging tolerance were shortlisted to evaluate their performance under two field screening conditions in two locations. The accessions EC471972, EC471920 and EC472119 were identified as tolerant to eight days of waterlogging in the seed germination stage after four levels of screening. These selected accessions can be used as sources of pregermination anaerobic stress tolerance in breeding programs.

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Data availability

Data is generated from inhouse project of Soybean in the Division of Genetics, Indian Agricultural Research Insitute (ICAR-IARI), New Delhi, India.

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The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Division of Genetics, ICAR-Indian Agricultural Research Institute (IARI), Pusa Campus, New Delhi, 110012, India

    Ambika Rajendran, Sanjay Kumar Lal & Deepshikha Sharma

  2. Division of Plant Physiology, ICAR-Indian Agricultural Research Institute (IARI), Pusa Campus, New Delhi, 110012, India

    Dhandapani Raju

  3. Division of Genetics, ICAR-Indian Agricultural Research Institute (IARI) Regional Station, Dharwad, Karnataka, 580005, India

    Bingi Pujari Mallikarjun

  4. School of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Georgetown, Penang, Malaysia

    Ayyagari Ramlal

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  1. Ambika Rajendran
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  2. Sanjay Kumar Lal
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  3. Dhandapani Raju
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  4. Bingi Pujari Mallikarjun
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  5. Ayyagari Ramlal
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  6. Deepshikha Sharma
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Contributions

Study conception and design by Ambika Rajendran and Dhandapani Raju. Material preparation, data collection and analysis were performed by Ambika Rajendran, Dhandapani Raju and some experimentation by Mallikarjun BP. The first draft of the manuscript was written by Ambika Rajendran and all authors commented on previous versions of the manuscript. Dhandapani Raju, Sanjay Kumar Lal and Mallikarjun BP reviewed the manuscript. Ayyagari Ramlal formatted the article. All authors read and approved the final manuscript.

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Correspondence to Ambika Rajendran.

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Rajendran, A., Lal, S.K., Raju, D. et al. Waterlogging tolerance evaluation methods for soybean (Glycine max (L.) Merr.) at the pregermination stage. Genet Resour Crop Evol 70, 2451–2461 (2023). https://doi.org/10.1007/s10722-023-01573-0

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