Abstract
Polygonatum cirrhifolium (Wall.) Royle is an important medicinal herb of the family Asparagaceae used to cure several ailments. The rhizome of the species forms an important ingredient of “Chyavanprash” which is identified for its rejuvenating properties. However, P. cirrhifolium is least explored scientifically and systematically till date. Therefore, in this study genetic diversity and phytochemical variations along with antimutagenic activity of P. cirrhifolium populations were evaluated. Antimutagenic activity varied remarkably (p < 0.05) among population and Gagar population with significantly (p < 0.05) higher DNA recovery (84.95%) percentage. Higher genetic diversity (He) was recorded among populations using RAPD (He, 0.30–0.36) and ISSR (0.25–0.38) markers. High intra and low inter population variations were recorded in the species using both kinds of markers. Phenolics (p < 0.05; r = 0.924); tannins (p < 0.05; r = 0.897) and DNA damage inhibition efficiency displayed a highly positive correlation with genetic diversity (estimated using ISSR markers). The population structure analysis of P. cirrhifolium revealed that the greatest value of the K was 3 for studied populations. Gene flow among studied populations was found sufficient to encounter genetic erosion in the species. Therefore, it is recommended that the populations with higher ingredient and genetic diversity can be utilized for conservation priority and management plan of this species.
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References
Azqueta A, Collins A (2016) Polyphenols and DNA damage: a mixed blessing. Nutrients 8:785
Balkrishna A, Srivastava A, Mishra RK, Patel SP, Vashistha RK, Singh A, Jadon V, Saxena P (2012) Astavarga plants-threatened medicinal herbs of the North-West Himalaya. Int J Medicinal Aromat Plants 2:661–676
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Annal Biochem 239:70–76. https://doi.org/10.1006/abio.1996.0292
Brand-Williams W, Cuvelier ME, Berset CLWT (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 28:25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Cai Y, Luo Q, Sun M, Corke H (2004) Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci 74:2157–2184. https://doi.org/10.1016/j.lfs.2003.09.047
Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178–182. https://doi.org/10.38212/2224-6614.2748
Chaudhary AA, Dhar U, Ahmad A, Bhatt ID, Jugran A, Kaur G (2012) Analysis of genetic diversity in Hedychium spicatum Buch. Ham. ex. D. Don in West Himalaya, India. J Med Plant Res 6:3984–3991. https://doi.org/10.5897/JMPR12.014
Chung MY, López-Pujol J, Chung JM, Kim KJ, Chung MG (2014) Contrasting levels of clonal and within-population genetic diversity between the 2 ecologically different herbs Polygonatum stenophyllum and Polygonatum inflatum (Liliaceae). J Hered 105:690–701. https://doi.org/10.1093/jhered/esu048
Ding G, Li X, Ding X, Qian L (2009) Genetic diversity across natural populations of Dendrobium officinale, the endangered medicinal herb endemic to China, revealed by ISSR and RAPD markers. Russ J Genet 45:327–334. https://doi.org/10.1134/S1022795409030119
Earl DA (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361. https://doi.org/10.1007/s12686-011-9548-7
Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individual using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x
Fang HL, Nie W, Zhu PL, Liang CY, Deng SY (2018) Genetic diversity in Callicarpa kwangtungensis Chun. based on morphological, biochemical and ISSR markers. J Appl Res Med Aromat Plants 10:41–48
Felsenstein J (2006) PHYLIP (phylogenetic inference package) documentation files, version 3.66. Department of Genome Sciences, University of Washington, Seattle, USA. https://doi.org/10.1016/j.jarmap.2018.06.001
Feng T, Jia Q, Meng X, Chen X, Wang F, Chai W, Liang Z (2020) Evaluation of genetic diversity and construction of DNA fingerprinting in Polygonatum Mill. based on EST-SSR and SRAP molecular markers. 3 Biotech. https://doi.org/10.1007/s13205-020-02316-z
Giri L, Belwal T, Bahukhandi A, Suyal R, Bhatt ID, Rawal RS, Nandi SK (2017) Oxidative DNA damage protective activity and antioxidant potential of Ashtvarga species growing in the Indian Himalayan Region. Ind Crops Prod 102:173–179. https://doi.org/10.1016/j.indcrop.2017.03.023
Hamrick JL, Godt MW (1990) Allozyme diversity in plant species. Plant population genetics, breeding, and genetic resources. Sinauer Associates Inc., Sunderland, pp 43–63
Hirai N, Miura T, Moriyasu M, Ichimaru M, Nishiyama Y, Ogura K, Kato A (1997) Cardiotonic activity of the rhizome of Polygonatum sibiricum in rats. Biol Pharm Bull 20:1271–1273. https://doi.org/10.1248/bpb.20.1271
Giri L, Jugran AK, Bahukhandi A, Dhyani P, Bhatt ID, Rawal RS, Nandi SK, Dhar U (2017a) Population genetic structure and marker trait associations using morphological, phytochemical and molecular parameters in Habenaria edgeworthii—a threatened medicinal orchid of West Himalaya, India. Appl Biochem Biotechnol 181:267–282. https://doi.org/10.1007/s12010-016-2211-8
Jugran AK, Bahukhandi A, Dhyani P, Bhatt ID, Rawal RS, Nandi SK (2016) Impact of altitudes and habitats on Valerenic acid, total phenolics, flavonoids, tannins, and antioxidant activity of Valeriana jatamansi. Appl Biochem Biotechnol 179:911–926. https://doi.org/10.1007/s12010-016-2039-2
Jugran AK, Bhatt ID, Rawal RS, Nandi SK, Pande V (2013) Patterns of morphological and genetic diversity of Valeriana jatamansi Jones in different habitats and altitudinal range of West Himalaya, India. Flora 208:13–21. https://doi.org/10.1016/j.flora.2012.12.003
Khan H, Saeed M, Gilani AH, Muhammad N, ur Rehman N, Mehmood MH, Ashraf N (2016) Antispasmodic and antidiarrheal activities of rhizomes of Polygonatum verticillatum maneuvered predominately through activation of K+ channels: components identification through TLC. Toxicol Ind Health 32:677–685. https://doi.org/10.1177/0748233713506956
Khan H, Saeed M, Mehmood MH, Rehman NU, Muhammad N, Haq IU, Ashraf N, El-Tahir KEH, Gilani AH (2013) Studies on tracheorelaxant and anti-inflammatory activities of rhizomes of Polygonatum verticillatum. BMC Complement Altern Med. https://doi.org/10.1186/1472-6882-13-197
Kramp K, Huck S, Niketic M, Tomovic G, Schmitt T (2009) Multiple glacial refugia and complex postglacial range shifts of the obligatory woodland plant Polygonatum verticillatum (Convallariaceae). Plant Biol 11:392–404. https://doi.org/10.1111/j.1438-8677.2008.00130.x
Kumaran A, Karunakaran RJ (2007) In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT-Food Sci Technol 40, 344–352
Lattoo SK, Khan S, Dhar AK (2005) A new chromosome number in Polygonatum cirrhifolium Royle: an endangered Liliaceous medicinal herb. Curr Sci 89: 1080–1081
Liu XX, Wan ZJ, Shi L, Lu XX (2011) Preparation and antiherpetic activities of chemical modified polysaccharides from Polygonatum cyrtonema Hua. Carbohydr Polym 83:737–742. https://doi.org/10.1016/j.carbpol.2010.08.044
Manners V, Kumaria S, Tandon P (2013) SPAR methods revealed high genetic diversity within populations and high gene flow of Vanda coerulea Griff ex Lindl (Blue Vanda), an endangered orchid species. Gene 519:91–97. https://doi.org/10.1016/j.gene.2013.01.037
Meng Y, Nie ZL, Deng T, Wen J, Yang YP (2014) Phylogenetics and evolution of phyllotaxy in the Solomon’s seal genus Polygonatum (Asparagaceae: Polygonateae). Bot J Linn Soc 176:435–451. https://doi.org/10.1111/boj.12218
Mucciarelli M, Ferrazzini D, Belletti P (2014) Genetic variability and population divergence in the rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as revealed by RAPD analysis. PLoS ONE. https://doi.org/10.1371/journal.pone.0101967
Naik PK, Alam MA, Singh H, Goyal V, Parida S, Kalia S, Mohapatra T (2010) Assessment of genetic diversity through RAPD, ISSR and AFLP markers in Podophyllum hexandrum: a medicinal herb from the Northwestern Himalayan region. Physiol Mol Biol Plants 16:135–148. https://doi.org/10.1007/s12298-010-0015-9
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590. https://doi.org/10.1093/genetics/89.3.583
Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155. https://doi.org/10.1111/j.1365-294X.2004.02141.x
Peakall ROD, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959. https://doi.org/10.1093/genetics/155.2.945
Rawat S, Andola H, Giri L, Dhyani P, Jugran A, Bhatt ID, Rawal RS (2013) Assessment of nutritional and antioxidant potential of selected vitality strengthening Himalayan medicinal plants. Int J Food Prop 17:703–712. https://doi.org/10.1080/10942912.2012.654563
Ray A, Jena S, Haldar T, Sahoo A, Kar B, Patnaik J, Ghosh B, Panda PC, Mahapatra N, Nayak S (2019) Population genetic structure and diversity analysis in Hedychium coronarium populations using morphological, phytochemical and molecular markers. Ind Crops Prod 132:118–133. https://doi.org/10.1016/j.indcrop.2019.02.015
Russo A, Cardile V, Caggia S, Gunther G, Troncoso N, Garbarino J (2011) Boldo prevents UV light and nitric oxide mediated plasmid DNA damage and reduces the expression of Hsp70 protein in melanoma cancer cells. J Pharm Pharmacol 63:1219–1229. https://doi.org/10.1111/j.2042-7158.2011.01320.x
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Singh SK, Patra A (2018) Evaluation of phenolic composition, antioxidant, anti-inflammatory and anticancer activities of Polygonatum verticillatum (L.). J Integr Med 16: 273–282
Singh SK, Patra A (2019) Evaluation of adaptogenic potential of Polygonatum cirrhifolium (Wall.) Royle: in vitro, in vivo and in silico studies. S Afr J Bot 121:159–177. https://doi.org/10.1016/j.sajb.2018.10.022
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16:144–158
Suyal R, Rawat S, Rawal RS, Bhatt ID (2019a) Variability in morphology, phytochemicals, and antioxidants in Polygonatum verticillatum (L.) All. populations under different altitudes and habitat conditions in Western Himalaya, India. Environ Monit Assess. https://doi.org/10.1007/s10661-019-7687-6
Suyal R, Bhatt D, Rawal RS, Tewari LM (2019b) Status of two threatened Astavarga herbs, Polygonatum cirrhifolium and Malaxis muscifera, in West Himalaya: conservation implications. Proc Natl Acad Sci India B Biol Sci. https://doi.org/10.1007/s40011-019-01144-3
Suyal R, Jugran AK, Rawal RS, Bhatt ID (2021) Morphological, phytochemical and genetic diversity of threatened Polygonatum verticillatum (L.) All. populations of different altitudes and habitat types in Himalayan region. Physiol Mol Biol Plants. https://doi.org/10.1007/s12298-021-01044-9
Tabin S, Kamili AN, Ganie SA, Zargar O, Sharma V, Gupta RC (2016) Genetic diversity and population structure of Rheum species in Kashmir Himalaya based on ISSR markers. Flora 223:121–128. https://doi.org/10.1016/j.flora.2016.05.001
Tiwari V, Mahar KS, Singh N, Meena B, Nair KN, Datt B, Upreti DK, Tamta S, Rana TS (2015) Genetic variability and population structure of Bergenia ciliata (Saxifragaceae) in the Western Himalaya inferred from DAMD and ISSR markers. Biochem Syst Ecol 60:165–170. https://doi.org/10.1016/j.bse.2015.04.018
Wang D, Song X, Li J (2006) Extraction and isolation technology of polysaccharides from Polygonatum cirrhifolium Root. J Northwest for Univ 21:158
Wang W, Dabu X, He J, Yang H, Yang S, Chen J, Fan W, Zhang G, Cai J, Ai H, Hai M (2019) Polygonatone H, a new homoisoflavanone with cytotoxicity from Polygonatum cyrtonema Hua. Nat Prod Res 33:1727–1733. https://doi.org/10.1080/14786419.2018.1434645
Wright S (1951) The genetical structure of populations. Ann Eugen 15:323–354. https://doi.org/10.1111/j.1469-1809.1949.tb02451.x
Xu Y, Lin W, Ye S, Wang H, Wang T, Su Y, Wu L, Wang Y, Xu Q, Cai J (2017) Protective effects of an ancient chinese kidney-tonifying formula against H2O2-induced oxidative damage to MES23.5 cells. Parkinson’s disease. https://doi.org/10.1155/2017/2879495
Yeh FC, Boyle T, Rongcai Y, Ye Z, Xian JM (1999) POPGENE, version 1.31. A Microsoft Window Based Freeware for Population Genetic Analysis. University of Alberta, Edmonton, Canada. https://www.ualberta.ca/fyeh/popgene.download.html
Zhang X, Kong W, Wang X, Zhang J, Liu L, Wang W, Zhang H, Deng Q (2020) Genetic diversity analysis of 34 fig varieties (Ficus carica L.) based on ISSR molecular marker. Genet Resour Crop Evol 67:913–921. https://doi.org/10.1007/s10722-020-00889-5
Zhao P, Zhao C, Li X, Gao Q, Huang L, Xiao P, Gao W (2018) The genus Polygonatum: a review of ethnopharmacology, phytochemistry and pharmacology. J Ethnopharmacol 214:274–291. https://doi.org/10.1016/j.jep.2017.12.006
Acknowledgements
The authors are thankful to Director, GBPNIHE, Kosi-Katarmal, Almora for facilities and encouragement. Help obtained from the members of BCM & BTA Group is highly acknowledged. This paper is the part of Ph. D. thesis of Dr. Renu Suyal and dedicated to Late Dr. R. S. Rawal for his supervision during the Ph. D. work.
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This work was supported under Botanical Garden Scheme of Ministry of Environment, Forest and Climate change (MoEF&CC), New Delhi (F.N. BSI-290/6/2013- Tech; Dated 29/09/2013) and Project No. 4 (In house project) funded to CBCM group by GB Pant National Institute of Himalayan Environment (MoEF&CC).
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RS, AKJ, and IDB conceptualized the study. RS and AKJ standardized methodology and conducted the experiments. AKJ performed data analysis. IBD and RSR contributed in management of resources and funds. RS and AKJ contributed in Writing—Original Draft of the MS. All authors contributed in Writing—Review & Editing of the manuscript.
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Suyal, R., Jugran, A.K., Bhatt, I.D. et al. Assessment of genetic diversity, population structure and phytochemical variations in Polygonatum cirrhifolium (Wall.) Royle: an endangered medicinal herb. Genet Resour Crop Evol 69, 2383–2397 (2022). https://doi.org/10.1007/s10722-022-01378-7
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DOI: https://doi.org/10.1007/s10722-022-01378-7