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Differentiation of Greek grapevine cultivars (Vitis vinifera L.) based on the combination of ampelographic description and microsatellite markers

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Abstract

Greek vineyards are characterized by a great number of indigenous grapevine cultivars as well as grapevine cultivars of eastern origin. Due to their age-old lineage and cultivation in Greece, most of those cultivars exhibit an even greater number of biotypes and mutations which frequently lead to an improper use of synonyms and homonyms. The aim of this study was the identification and discrimination of twelve grapevine cultivars cultivated in Greece by means of the seventy-four OIV descriptors, followed by the molecular analysis of five microsatellite markers on each grapevine biotype and cultivar during the years 2015, 2016, and 2017, on the basis of a list of ampelographic descriptors developed by the International Organization of Vine and Wine (OIV in OIV descriptor list for grape varieties and Vitis species, 2nd edn, Organisation Internationale de la Vigne et du Vin, 2009. http://oiv.int/en/technical-standards-and-documents/description-of-grape-varieties), followed by the molecular analysis of five microsatellite markers. The studied grapevine cultivars were clustered according to their origin; grapevine cultivars of eastern origin were placed within the same cluster of the dendrogram, while the high degree of genetic similarity of grapevine cultivars ‘Avgoulato’, ‘Korithi lefko’, ‘Kolokythas’ and ‘Baresana’ bear to one another corroborates the hypothesis that all of them were formed by mutations from the same single cultivar. With regard to the indigenous grapevine cultivars, ‘Zalovitiko’ proved to be a synonym of ‘Xinomavro’, while the low degree of genetic similarity between grape cultivars ‘Korithi mavro’ and ‘Korithi lefko’ suggests that they are different and do not constitute colour mutations, as it had been believed so far. In most cases, the molecular findings confirmed the results of the ampelographic description. More importantly, they indicated that only the combination of the ampelographic description with molecular markers can allow for a more effective identification and discrimination of grapevine cultivars.

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References

  • Arroyo-García R, Ruiz-García L, Bolling L, Ocete R, López MA, Arnold C, Ergul A, Söylemezoflu G, Uzun HI, Cabello F, Ibáñez J, Aradhya MK, Atanassov A, Atanassov I, Balint S, Cenis JL, Costantini L, Gorislavets S, Grando MS, Klein BY, McGovern PE, Merdinoglu D, Pejic I, Pelsy F, Primikirios N, Risovannaya V, Roubelakis-Angelakis KA, Snoussi H, Sotiri P, Tamhankar S, This P, Troshin L, Malpica JM, Lefort F, Martinez-Zapater JM (2006) Multiple origins of cultivated grapevine (Vitis vinifera L., ssp sativa) based on chloroplast DNA polymorphisms. Mol Ecol 15(12):3707–3714

    Article  Google Scholar 

  • Avise JC (2001) Molecular markers, natural history and evolution, 4th Printing. Kluwer, Dordrecht

  • Babiniotis G (2009) Etymological dictionary of modern Greek (in Greek). Lexicology Centre, Athens

    Google Scholar 

  • Biniari K (2005) Identification and genetic study of grapevine cultivars by random amplified polymorphic DNA markers (PCR). Dissertation, Agricultural University of Athens

  • Biniari K, Stavrakaki M (2019) Genetic study of native grapevine varieties of northern, western and central Greece with the use of ampelographic and molecular methods. Not Bot Horti Agrobo. https://doi.org/10.15835/nbha47111213

    Article  Google Scholar 

  • Bitz L, Zinelabidine LH, Ruehl E, Bitz O (2015) Microsatellite analysis of traditional eastern grapevine varieties and wild accessions from Geisenheim collection in Germany. Vitis 54:17–21

    Google Scholar 

  • Bowers JE, Dangl GS, Vignani R, Meredith CP (1996) Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome 39:628–633

    Article  CAS  Google Scholar 

  • Celik H, Karli A(2003) The use of in ovulo embryo culture for cross-breeding studies of empty—seeded table grape cultivars. In: Hajdu E, Borbás É (eds) Proceedings of VIIIth IC on grape genetics and breeding. Acta Hortic 603, ISHS 2003

  • Davidis OX (1982) Greek Ampelology, Volume 3, Elements of Ampelography (in Greek). Agricultural University of Athens, University Press, Athens

    Google Scholar 

  • De Andrés MT, Benito A, Perez-Rivera G, Ocete R, Lopez MA, Gaforio L, Muñoz G, Cabello F, Martínez-Zapater JM, Arroyo-García R (2012) Genetic diversity of wild grapevine populations in Spain and their genetic relationship with cultivated grapevines. Mol Ecol 21(4):800–816

    Article  Google Scholar 

  • Fregoni M (1991) Origines de la Vigne et de la Viticulture. Musumeci Editeur, Quart (Vale d’Aosta), Italy

  • Galet P (2000) Dictionnaire Encyclopédique des Cépages. Hachette pratique, Paris

    Google Scholar 

  • Grassi F, Labra M, Imazio S, Spada A, Sgorbati S, Scienza A, Sala F (2003) Evidence of a secondary grapevine domestication centre detected by SSR analysis. Theor Appl Genet 107:1315–1320. https://doi.org/10.1007/s00122-003-1321-1

    Article  CAS  PubMed  Google Scholar 

  • Guillon JM (1896) Les cépages orientaux. G. Carre, Paris

    Google Scholar 

  • Katramis N (1880) Philological analects of Zante (in Greek). Zante, Greece

    Google Scholar 

  • Kourakou S (2017) Xinomavro. The wine-grape variety of Central-western Macedonia. Publications of Foinikas, Athens

    Google Scholar 

  • Krimbas V (1943) Greek Ampelography, vol I (in Greek). Ministry of Agriculture, Athens

    Google Scholar 

  • Krimbas V (1944) Greek Ampelography, vol II (in Greek). Ministry of Agriculture, Athens

    Google Scholar 

  • Lefort F, Roubelakis-Angelakis KA (2001) Genetic comparison of Greek cultivars of Vitis vinifera L. by nuclear microsatellite profiling. Am J Enol Vitic 52(2):101–108

    Google Scholar 

  • Levadoux LD (1956) Wild and cultivated populations of Vitis vinifera L. Ann Amel Plant 6:59–118

    Google Scholar 

  • Logothetis BX (1970) The development of the vine and of viticulture in Greece, based on archeological findings in the area. Aristotle University of Thessaloniki, Thessaloniki

    Google Scholar 

  • Logothetis BX (1975) Contributions de la vigne et du vin à la civilisation de la Grèce et de la Méditerranée orientale. Annuaire de la Faculté Agronomique de l’Université de Thessaloniki 17:1–286

    Google Scholar 

  • Marshall TC, Slate J, Kruuk LE, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655

    Article  CAS  Google Scholar 

  • Merkouropoulos G, Michailidou S, Alifragkis A, Zioziou E, Koundouras S, Argiriou A, Nikolaou N (2015) A combined approach involving ampelographic description, berry oenological traits and molecular analysis to study native grapevine varieties of Greece. Vitis 54:99–103

    Google Scholar 

  • Negrul AM (1938) Evolution of cultivated forms of grapes. C R Acad Sci USSR 18:585–588

    Google Scholar 

  • Negrul AM (1946) Origin of the cultivated grapevine and its classification. In: Baranoc A, Kai YF, Lazarevski MA, Negrul AM, Palibin TV, Prosmoserdov NN (eds)Ampelographia of USSR, vol. I. Moscow, Russia, pp 159–216

  • OIV (2009) OIV descriptor list for grape varieties and Vitis species, 2nd edn. Organisation Internationale de la Vigne et du Vin. Retrieved March 4, 2015 from http://oiv.int/en/technical-standards-and-documents/description-of-grape-varieties

  • OIV (2013) OIV international list of vine varieties and their synonyms. Organisation Internationale de la Vigne et du Vin. Retrieved March 4, 2015 from http://oiv.int/en/technical-standards-and-documents/description-of-grape-varieties

  • Palaiologos Gr (1836) On viticulture and enology (in Greek). Royal Printing, Athens

    Google Scholar 

  • Poniropoulos E (1888) Viticulture and enology (in Greek). N. Michalopoulos, Athens

    Google Scholar 

  • Pulliat V (1888) Mille variétés de vignes. Description et synonymies, 3ème ed. Coulet, Montpellier

  • Rohlf FJ (2000) NTSYS-pc. Numerical taxonomy and multivariate analysis system (Version 2.1). Exeter Software, Setauket, New York

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Schneider A, Raimondi S, Pirolo CS, Marinoni DT, Ruffa P, Venerito P, La Notte P (2014) Genetic characterization of grape cultivars from Apulia (Southern Italy) and synonymies in other mediterranean regions. Am J Enol Vitic 65(2):244–249. https://doi.org/10.5344/ajev.2013.13082

    Article  Google Scholar 

  • Sefc KM, Lefort F, Grando MS, Scott KD, Steinkellner H, Thomas MR (2001) Microsatellite markers for grapevine. A state of the art. In: Roubelakis-Angelakis KA (ed) Molecular biology and biotechnology of the grapevine. Kluwer, Dordrecht, pp 433–463

    Chapter  Google Scholar 

  • Sibthorp J, Smith EJ (1806–1809) Florae Graecae prodromus; sive Plantarum omnium enumeratio, vol 1 and 2. Typis Richardi Taylor et Socii, in vico Shoe Lane, veneunt apud Johannem White, in vico Fleet Street, Londini

  • Stavrakaki M, Biniari K (2016) Genotyping and phenotyping of twenty old traditional Greek grapevine varieties (Vitis vinifera L.) from Eastern and Western Greece. Sci Hortic 209:86–95. https://doi.org/10.1016/j.scienta.2016.06.021

    Article  Google Scholar 

  • Stavrakakis MN, Biniari K (1998/9) Genetic study of the grape cultivar Rhoditis by random amplified polymorphic DNA markers. Agric Res 220:45–52

  • Stavrakakis MN, Stavrakaki M, Biniari K, Bouza D (2019) Ampelography of the forgotten Greek grapevine cultivars (in Greek). Embryo Publications, Athens (in press)  

    Google Scholar 

  • This P, Martínez-Zapater JM, Peros JP, Lacombe T (2011) Natural variation in Vitis. In: Adam-Blondon AF et al (eds) Genetics, genomics, and breeding of grapes. CRC Press, Boca Raton, pp 30–67

    Chapter  Google Scholar 

  • Viala P, Vermorel V (1909) Traité Général d’Ampélographie (Volumes 7). Maison éd, Paris

  • Vlachos M (1986) Ampelography (in Greek). Thessaloniki

  • Vouillamoz JF, McGovern PE, Ergül A, Söylemezoglu G, Tevzadze G, Meredith CP, Grando MS (2006) Genetic characterization and relationships of traditional grape cultivars from Transcaucasia and Anatolia. Plant Genet Resour 4(2):144–158

    Article  CAS  Google Scholar 

  • Walpole RMA (1818) Memoirs relating to European and Asiatic Turkey, and other countries of the East, 2nd edn. Longman, Hurst, Rees, Orme, and Brown, London

    Book  Google Scholar 

  • Zois LX (1898) Filological and historical dictionary of Zante (in Greek), vol I. O. Foskolos. Zante, Greece

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Viticulture, Faculty of Crop Science, Agricultural University of Athens, 75 Iera Odos Street, 11855, Athens, Greece

    Maritina Stavrakaki, Despoina Bouza & Katerina Biniari

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  1. Maritina Stavrakaki
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  2. Despoina Bouza
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  3. Katerina Biniari
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Correspondence to Maritina Stavrakaki.

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Appendices

Appendix 1: Grapevine cultivars studied

(i) Grapevine cultivars of eastern origin

figure a

(ii) Grapevine cultivar Baresana and indigenous Greek grapevine cultivars

figure b

Appendix 2

See Table 5.

Table 5 Ampelographic descriptors, based on the OIV descriptors list (OIV descriptors list, 2009)
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Appendix 3

See Table 6.

Table 6 Ampelographic characterization of the cultivars studied, based on the 74 OIV descriptors used (OIV descriptors list, 2009)
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Appendix 4

See Table 7.

Table 7 Genetic characterization (allele size) of the cultivars studied, based on the five molecular markers used
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Stavrakaki, M., Bouza, D. & Biniari, K. Differentiation of Greek grapevine cultivars (Vitis vinifera L.) based on the combination of ampelographic description and microsatellite markers. Genet Resour Crop Evol 67, 21–40 (2020). https://doi.org/10.1007/s10722-019-00860-z

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