Abstract.
The origin of the grapevine was investigated with archaeobotanical, cultural and historical data. A primary domestication centre was located in the Near East region but there is no agreement on the existence or role of secondary domestication centres. In this work, PCR-based microsatellite analysis has been applied to study the origin of some Italian cultivated grapevines from in situ direct domestication of the wild autoctonous grapevine. Three different Italian locations in Grosseto, Cosenza and Nuoro were identified for this study, and domesticated grapevine as well as wild local accessions growing in these location, were analysed by SSR markers. Cluster analysis performed on Cosenza and Grosseto samples showed a high value of genetic distance between domesticated and wild accessions. On the contrary two cultivars (Bovale Murru and Bovale Muristellu) recovered in Nuoro (in the Sardinia island) were very close to some wild varieties. This suggests that the latter two cultivars may have originated from wild grapevines and consequently that in this location a secondary grapevine domestication event occurred. Six Lambrusco varieties were also included in this analysis as ancient putative ancestors of the cultivated grapevines. The molecular analysis excluded this hypothesis and suggest Lambrusco as an independent Vitis taxon.
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Acknowledgements.
We are grateful to Prof. Francesco Salamini for perceptive comments that helped to improve this manuscript and anonymous referees for important comments and suggestions. This research was supported by the project "Biodiversity of the Italian grapevines and its protection from pathogens" of Fondazione Bussolera Branca, Mairano di Casteggio, Pavia, Italy. Finally, we thank Roberto Brontini and Riccardo Cop for technical support.
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Grassi, F., Labra, M., Imazio, S. et al. Evidence of a secondary grapevine domestication centre detected by SSR analysis. Theor Appl Genet 107, 1315–1320 (2003). https://doi.org/10.1007/s00122-003-1321-1
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DOI: https://doi.org/10.1007/s00122-003-1321-1