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A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke

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Abstract

The Dalbergieae lectin group encompasses several lectins with significant differences in their carbohydrate specificities and biological properties. The current work reports on the purification and characterization of a GalNAc/Gal-specific lectin from Vataireopsis araroba (Aguiar) Ducke, designated as VaL. The lectin was purified from the seeds in a single step using guar gum affinity chromatography. The lectin migrated as a single band of about 35 kDa on SDS-PAGE and, in native conditions, occurs as a homodimer. The purified lectin is stable at temperatures up to 60 °C and in a pH range from 7 to 8 and requires divalent cations for its activity. Sugar-inhibition assays demonstrate the lectin specificity towards N-acetyl-D-galactosamine, D-galactose and related sugars. Furthermore, glycan array analyses show that VaL interacts preferentially with glycans containing terminal GalNAc/Galβ1-4GlcNAc. Biological activity assays were performed using three insect cell lines: CF1 midgut cells from the spruce budworm Choristoneura fumiferana, S2 embryo cells from the fruit fly Drosophila melanogaster, and GutAW midgut cells from the corn earworm Helicoverpa zea. In vitro assays indicated a biostatic effect for VaL on CF1 cells, but not on S2 and GutAW cells. The lectin presented a biostatic effect by reducing the cell growth and inducing cell agglutination, suggesting an interaction with glycans on the cell surface. VaL has been characterized as a galactoside-specific lectin of the Dalbergieae tribe, with sequence similarity to lectins from Vatairea and Arachis.

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Acknowledgements

This study was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP). B.S.C and K.S.N are senior investigators of CNPq. N. R. and S.S. are grateful to Ministerio de Ciencia e Innovación and Agencia Estatal de Investigación (MCIN/AEI: https://doi.org/10.13039/501100011033) for funding received under grant number PID2020-117552RB-I00 and the Maria de Maeztu Units of Excellence Programme (Grant MDM-2017-0720). VJSO is grateful to FWO-Vlaanderen for the post-doctoral fellowship (12T4622N).

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Authors and Affiliations

  1. Laboratory for Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, 9000, Ghent, Belgium

    Vinicius J.S. Osterne & Els J.M. Van Damme

  2. Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceara, 60455-760, Fortaleza, Brazil

    Messias V. Oliveira, Benildo S. Cavada & Kyria Santiago Nascimento

  3. Laboratory of Agrozoology, Department of Plants and Crops, Ghent University, 9000, Ghent, Belgium

    Kristof De Schutter & Guy Smagghe

  4. Glycotechnology Laboratory, Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo Miramon 182, 20014, San Sebastian, Spain

    Sonia Serna & Niels-Christian Reichardt

  5. CIBER-BBN, 20009, San Sebastian, Spain

    Niels-Christian Reichardt

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Osterne, V.J., Oliveira, M.V., De Schutter, K. et al. A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke. Glycoconj J 40, 85–95 (2023). https://doi.org/10.1007/s10719-022-10082-8

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