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Comparative transcriptome analysis of eyes reveals the adaptive mechanism of mantis shrimp (oratosquilla oratoria) induced by a dark environment

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Abstract

The light-dark cycle significantly impacts the growth and development of animals. Mantis shrimps (Oratosquilla oratoria) receive light through their complex photoreceptors. To reveal the adaptive expression mechanism of the mantis shrimp induced in a dark environment, we performed comparative transcriptome analysis with O. oratoria cultured in a light environment (Oo-L) as the control group and O. oratoria cultured in a dark environment (Oo-D) as the experimental group. In the screening of differentially expressed genes (DEGs) between the Oo-L and Oo-D groups, a total of 88 DEGs with |log2FC| > 1 and FDR < 0.05 were identified, of which 78 were upregulated and 10 were downregulated. Then, FBP1 and Pepck were downregulated in the gluconeogenesis pathway, and MKNK2 was upregulated in the MAPK classical pathway, which promoted cell proliferation and differentiation, indicating that the activity of mantis shrimp was slowed and the metabolic rate decreases in the dark environment. As a result, the energy was saved for its growth and development. At the same time, we performed gene set enrichment analysis (GSEA) on all DEGs. In the KEGG pathway analysis, each metabolic pathway in the dark environment showed a slowing trend. GO was enriched in biological processes such as eye development, sensory perception and sensory organ development. The study showed that mantis shrimp slowed down metabolism in the dark, while the role of sensory organs prominent. It provides important information for further understanding the energy metabolism and has great significance to study the physiology of mantis shrimp in dark environment.

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Data Availability

The datasets generated during the current study are available in the NCBI repository. The light environment (Oo-L) and dark environment (Oo-D) transcriptome data generated in this study were under project PRJNA903491, SRA : SRP409162. The raw reads of the full-length transcriptome sequenced by the Pacbio platform was under the project PRJNA1020148, SRA: SRR26151500, and the assembled transcriptome was under the project PRJNA1020076, SRA: SRR26145030.

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Acknowledgements

The work was funded by National Natural Science Foundation of China (32070526) and sponsored by “Qing Lan Project” & “333 Project” & “Outstanding Young Talents of YCTU”.

Funding

The work was funded by National Natural Science Foundation of China (32070526).

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Yancheng Teachers University, Yancheng, 224051, China

    Xiaoli Sun, Ling He, Bujin Ayi, Yuyang Qiu, Jiayue Xu, Wei Yu, Tinghao Yan, Boping Tang, Gang Wang & Daizhen Zhang

  2. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China

    Xiaoli Sun

  3. Chemical and Biological Engineering College, Yancheng Institute of Technology, Yancheng, 224003, China

    Ge Ding

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  1. Xiaoli Sun
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  4. Yuyang Qiu
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  10. Gang Wang
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Contributions

All authors contributed to the study conception and design. Methodology was performed by Gang Wang, Yuyang Qiu and Bujin Ayi. Formal analysis, data curation and investigation were performed by Tinghao Yan, Wei Yu and Jiayue Xu. Visualization was performed by Ling He. The original draft was written by Ge Ding and Daizhen Zhang. Writing-review and editing were performed by Xiaoli Sun and Boping Tang. All authors read and approved the final manuscript.

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Correspondence to Daizhen Zhang.

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This article does not contain any studies with human participants performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Sun, X., He, L., Ayi, B. et al. Comparative transcriptome analysis of eyes reveals the adaptive mechanism of mantis shrimp (oratosquilla oratoria) induced by a dark environment. Genetica 151, 339–348 (2023). https://doi.org/10.1007/s10709-023-00198-6

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