Abstract
Sustainable zinc (Zn) management strategies are needed to improve cereal yield production, particularly under tropical savannah conditions. Inoculation with plant growth-promoting bacteria, such as Azospirillum brasilense, could be a useful and sustainable strategy to enhance agronomic Zn use efficiency (AZnUE), leading to better cereal development. This study was developed to explore the effect of seed inoculation with A. brasilense combined with Zn application rates on maize-wheat crop development, AZnUE and grain yield. The field experiments were conducted during four cropping seasons, using five Zn application rates (0–8 kg Zn ha−1), with maize grown in the first season, and two seed inoculation treatments (without or with A. brasilense) applied to both maize and wheat crops. Our results showed that inoculation was effective in promoting maize and wheat development with higher AZnUE (an increase of 76% and 17% for maize and wheat, respectively), leading to a greater yield (an increase of 6% for the maize and wheat harvests). The estimated applied Zn rates (between 4 and 5 kg Zn ha−1) resulted in higher maize-wheat grain yields. Our study explored new perspectives of A. brasilense inoculation related to nutrient uptake and use efficiency under different seasonal climatic conditions. First, we examined the positive effect of A. brasilense on maize and wheat Zn uptake and AZnUE in tropical Zn-deficient soils. In addition, we examined the positive effect of A. brasilense inoculation on maize and wheat development and grain yield under harsh climatic conditions such as in the Brazilian savannah.
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References
Alloway BJ (2008) Zinc in soils and crop nutrition, 2nd edn. IFA, Paris, p 135
Amanullah I, Alwahibi MS, Elshikh MS, Alkahtani J, Muhammad A, Khalid S, Imran Ahmad M, Khan N, Ullah S, Ali I (2020) Phosphorus and zinc fertilization improve zinc biofortification in grains and straw of coarse vs. fine rice genotypes. Agronomy 10:1155. https://doi.org/10.3390/agronomy10081155
Bashan Y, de de-Bashan LE (2010) How the plant growth-promoting bacterium Azospirillum promotes plant growth—a critical assessment. Adv Agron 108:77–136. https://doi.org/10.1016/S0065-2113(10)08002-8
Bremner JM, Breitenbeck GA (1983) A simple method for determination of ammonium in semimicro-Kjeldahl analysis of soils and plant materials using a block digester. Commun Soil Sci Plant Anal 14:905–913. https://doi.org/10.1080/00103628309367418
Cakmak I (2008) Enrichment of cereal grains with zinc: agronomic or genetic biofortification? Plant Soil 302:1–17. https://doi.org/10.1007/s11104-007-9466-3
Cakmak I, Kutman UB (2017) Agronomic biofortification of cereals with zinc: a review. Eur J Soil Sci 69:172–180. https://doi.org/10.1111/ejss.12437
Cakmak I, McLaughlin MJ, White P (2017) Zinc for better crop production and human health. Plant Soil 411:1–4. https://doi.org/10.1007/s11104-016-3166-9
Cardillo BES, Oliveira DP, Soares BL, Martins FAD, Rufini M, Silva JS, Ferreira Neto GG, de Andrade MJB, Moreira FMDS (2019) Nodulation and yields of common bean are not affected either by fungicides or by the method of inoculation. Agron J 111:694–701. https://doi.org/10.2134/agronj2018.06.0389
Cassán F, Diaz-Zorita M (2016) Azospirillum sp. in current agriculture: from the laboratory to the field. Soil Biol Biochem 103:117–130. https://doi.org/10.1016/j.soilbio.2016.08.020
Cassán F, Perrig D, Sgroy V, Masciarelli O, Penna C, Luna V (2009) Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination and early seedling growth in corn (Zea mays L.) and soybean (Glycine max L.). Eur J Soil Biol 45:28–35. https://doi.org/10.1016/j.ejsobi.2008.08.005
Cassán F, Coniglio A, López G, Molina R, Nievas S, de Carlan CLN, Donadio F, Torres D, Rosas S, Pedrosa FO, de Souza E, Díaz Zorita M, de-Bashan L, Mora V (2020) Everything you must know about Azospirillum and its impact on agriculture and beyond. Biol Fertil Soils 56:461–479. https://doi.org/10.1007/s00374-020-01463-y
Cherubin MR, Oliveira DMS, Feigl BJ, Pimentel LG, Lisboa IP, Gmach MR, Varanda LL, Moraes MC, Satiro LS, Popin GV, Paiva SR, Santos AKB, Vasconcelos ALS, Melo PLA, Cerri CEP, Cerri CC (2018) Crop residue harvest for bioenergy production and its implications on soil functioning and plant growth: a review. Scientia Agricola 75:255–272. https://doi.org/10.1590/1678-992x-2016-0459
Conab. Companhia Nacional de Abastecimento (2020) Grains report—October 2020. Brasília: Conab (in Portuguese). https://www.conab.gov.br/info-agro/safras
Davarpanah S, Tehranifar A, Davarynejad G, Abadía J, Khorasani R (2016) Effects of foliar applications of zinc and boron nano-fertilizers on pomegranate (Punica granatum cv. Ardestani) fruit yield and quality. Sci Hortic 210:57–64. https://doi.org/10.1016/j.scienta.2016.07.003
de-Bashan LE, Hernandez J-P, Bashan Y (2012) The potential contribution of plant growth-promoting bacteria to reduce environmental degradation—a comprehensive evaluation. Appl Soil Ecol 61:171–189. https://doi.org/10.1016/j.apsoil.2011.09.003
Dhillon J, Torres G, Driver E, Figueiredo B, Raun WR (2017) World phosphorus use efficiency in cereal crops. Agron J 109:1670–1677. https://doi.org/10.2134/agronj2016.08.0483
Döbereiner J, Baldani VLD, Baldani JI (1995) How to isolate and identify diazotrophic bacteria from non-leguminous plants. Brasília, Embrapa-SPI e Seropédica, Embrapa-CNPAB, 60 p (in Portuguese).
Drissi S, Houssa AA, Bamouh A, Benbella M (2017) Response of corn silage (Zea mays L.) to zinc fertilization on a sandy soil under field and outdoor container conditions. J Saudi Soc Agric Sci 16:145–153. https://doi.org/10.1016/j.jssas.2015.05.002
Duca D, Lorv J, Patten CL, Rose D, Glick BR (2014) Indole-3-acetic acid in plantmicrobe interactions. Antonie Van Leeuwenhoek 106:85–125. https://doi.org/10.1007/s10482-013-0095-y
El-Esawi MA, Al-Ghamdi AA, Ali HM, Alayafi AA (2019) Azospirillum lipoferum FK1 confers improved salt tolerance in chickpea (Cicer arietinum L.) by modulating osmolytes, antioxidant machinery and stress-related genes expression. Environ Exp Bot 159:55–65. https://doi.org/10.1016/j.envexpbot.2018.12.001
Eurostat. European Statistics (2020) Agriculture, forestry and fishery statistics. http://ec.europa.eu/eurostat/statistics-explained/index.php/Agriculture,_forestry_and_fishery_statistics. Accessed 20 Oct 2020
FAO. United Nations Food and Agricultural Organization (2017) Wheat yields global by country. http://www.fao.org/faostat/en/#data. Accessed 20 Oct 2020
Fox A, Suter M, Widmer F, Lüscher A (2020) Positive legacy effect of previous legume proportion in a ley on the performance of a following crop of Lolium multiflorum. Plant Soil 447:497–506. https://doi.org/10.1007/s11104-019-04403-4
Fróna D, Szenderák J, Harangi-Rákos M (2019) The challenge of feeding the world. Sustainability 11:5816. https://doi.org/10.3390/su11205816
Fukami J, Ollero FJ, Megías M, Hungria M (2017) Phytohormones and induction of plant-stress tolerance and defense genes by seed and foliar inoculation with Azospirillum brasilense cells and metabolites promote maize growth. AMB Express 7:153–163. https://doi.org/10.1186/s13568-017-0453-7
Fukami J, Cerezini P, Hungria M (2018a) Azospirillum: benefits that go far beyond biological nitrogen fixation. AMB Express 8:73. https://doi.org/10.1186/s13568-018-0608-1
Fukami J, Abrantes JLF, Del Cerro P, Nogueira MA, Ollero FJ, Megías M, Hungria M (2018b) Revealing different strategies of quorum sensing in Azospirillum brasilense strains Ab-V5 and Ab-V6. Arch Microbiol 200:47–56. https://doi.org/10.1007/s00203-017-1422-x
Fukami J, De La Osa C, Ollero FJ, Megías M, Hungria M (2018c) Coinoculation of maize with Azospirillum brasilense and Rhizobium tropici as a strategy to mitigate salinity stress. Funct Plant Biol 45:328–339. https://doi.org/10.1071/FP17167
Galindo FS, Teixeira Filho MCM, Buzetti S, Pagliari PH, Santini JMK, Alves CJ, Megda MM, Nogueira TAR, Andreotti M, Arf O (2019) Maize yield response to nitrogen rates and sources associated with Azospirillum brasilense. Agron J 111:1985–1997. https://doi.org/10.2134/agronj2018.07.0481
Galindo FS, Buzetti S, Rodrigues WL, Boleta EHM, Silva VM, Tavanti RFR, Fernandes GC, Biagini ALC, Rosa PAL, Teixeira Filho MCM (2020a) Inoculation of Azospirillum brasilense associated with silicon as a liming source to improve nitrogen fertilization in wheat crops. Sci Rep 10:6160. https://doi.org/10.1038/s41598-020-63095-4
Galindo FS, Pagliari PH, Buzetti S, Rodrigues WL, Santini JMK, Boleta EHM, Rosa PAL, Nogueira TAR, Lazarini E, Teixeira Filho MCM (2020b) Can silicon applied to correct soil acidity in combination with Azospirillum brasilense inoculation improve nitrogen use efficiency in maize? PLoS ONE 15:e0230954. https://doi.org/10.1371/journal.pone.0230954
Gomes LC, Faria RM, de Souza E, Veloso GV, Schaefer CEG, Fernandes Filho EI (2019) Modelling and mapping soil organic carbon stocks in Brazil. Geoderma 340:337–350. https://doi.org/10.1016/j.geoderma.2019.01.007
Goswami D, Thakker JN, Dhandhukia PC, Tejada Moral M (2016) Portraying mechanics of plant growth promoting rhizobacteria (PGPR): a review. Cogent Food Agric 2:1127500. https://doi.org/10.1080/23311932.2015.1127500
Hansch R, Mendel RR (2009) Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Cur Opin Plant Biol 12:259–266. https://doi.org/10.1016/j.pbi.2009.05.006
Hatfield JL, Sauer TJ, Cruse RM (2017) Chapter one—soil: the forgotten piece of the water, food, energy Nexus. In: Sparks DL (ed) Advances in agronomy. Academic Press, Cambridge, pp 1–46
Housh AB, Powell G, Scott S, Anstaett A, Gerheart A, Benoit M, Waller S, Powell A, Guthrie JM, Higgins B, Wilder SL, Schueller MJ, Ferrieri RA (2021) Azospirillum brasilense bacterium elicits beneficial physiological and metabolic responses in Zea mays that contribute to increased host iron assimilation. ISME J. https://doi.org/10.1038/s41396-020-00866-x
Hungria M, Campo RJ, Souza EM, Pedrosa FO (2010) Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil. Plant Soil 331:413–425. https://doi.org/10.1007/s11104-009-0262-0
Hungria M, Ribeiro RA, Nogueira MA (2018) Draft genome sequences of Azospirillum brasilense strains Ab-V5 and Ab-V6, commercially used in inoculants for grasses and legumes in Brazil. Genome Announc 6:e00393-e418. https://doi.org/10.1128/genomeA.00393-18
Iannetta PPM, Young M, Bachinger J, Bergkvist G, Doltra J, Lopez-Bellido RJ, Monti M, Pappa VA, Reckling M, Topp CFE, Walker RL, Rees RM, Watson CA, James EK, Squire GR, Begg GS (2016) A comparative nitrogen balance and productivity analysis of legume and non-legume supported cropping systems: the potential role of biological nitrogen fixation. Front Plant Sci 7:1700. https://doi.org/10.3389/fpls.2016.01700
Jacoby R, Peukert M, Succurro A, Koprivova A, Kopriva S (2017) The role of soil microorganisms in plant mineral nutrition—current knowledge and future directions. Front Plant Sci 8:1617. https://doi.org/10.3389/fpls.2017.01617
Jalal A, Shah S, Teixeira Filho MCM, Khan A, Shah T, Ilyas M, Rosa PAL (2020) Agro-biofortification of zinc and iron in wheat grains. Gesunde Pflanzen 72:227–236. https://doi.org/10.1007/s10343-020-00505-7
Joy EJM, Ahmad W, Zia MH, Kumssa DB, Young SD, Ander EL, Watts MJ, Stein AJ, Broadley MR (2016) Valuing increased zinc (Zn) fertilizer-use in Pakistan. Plant Soil 411:139–150. https://doi.org/10.1007/s11104-016-2961-7
Kim Y-C, Glick BR, Bashan Y, Ryu C-M (2012) Enhancement of plant drought tolerance by microbes. In: Aroca R (ed) Plant responses to drought stress: from morphological to molecular features. Springer, Berlin, pp 383–413
Knapp S, van der Heijden MGA (2018) A global meta-analysis of yield stability in organic and conservation agriculture. Nat Commun 9:3632. https://doi.org/10.1038/s41467-018-05956-1
Korver RA, Koevoets IK, Testerink C (2018) Out of shape during stress: a key role for auxin. Trends Plant Sci 23:783–793. https://doi.org/10.1016/j.tplants.2018.05.011
Malavolta E, Vitti GC, Oliveira SA (1997) Evaluation of the nutritional status of plants: Principles and applications, 2 edn. Piracicaba, Potafos, 319 p (in Portuguese).
Manzeke GM, Mtambanengwe F, Nezomba H, Mapfumo P (2014) Zinc fertilization influence on maize productivity and grain nutritional quality under integrated soil fertility management in Zimbabwe. Field Crops Res 166:128–136. https://doi.org/10.1016/j.fcr.2014.05.019
Manzeke MG, Mtambanengwe F, Nezomba H, Watts MJ, Broadley MR, Mapfumo P (2017) Zinc fertilization increases productivity and grain nutritional quality of cowpea (Vigna unguiculate [L.] Walp.) under integrated soil fertility management. Field Crops Res 213:231–244. https://doi.org/10.1016/j.fcr.2017.08.010
Marschner P (2012) Marschner’s mineral nutrition of higher plants, 3rd edn. Academic Press, New York, p 651
Martins MR, Jantalia CP, Reis VM, Döwich I, Polidoro JC, Alves BJR, Boddey RM, Urquiaga S (2018) Impact of plant growth-promoting bacteria on grain yield, protein content, and urea-15 N recovery by maize in a Cerrado Oxisol. Plant Soil 422:239–250. https://doi.org/10.1007/s11104-017-3193-1
Moll RH, Kamprath EJ, Jackson WA (1982) Analysis and interpretation of factors which contribute to efficiency of nitrogen utilization. Agron J 74:562–564. https://doi.org/10.2134/agronj1982.00021962007400030037x
Munareto JD, Martin TN, Muller TM, Nunes UR, Rosa GB, Grando LFT (2018) Compatibility of Azospirillum brasilense with fungicide and insecticide and its effects on the physiological quality of wheat seeds. Semina: Ci Agr 39:855–864. https://doi.org/10.5433/1679-0359.2018v39n2p855
Nóia Junior RS, Sentelhas PC (2019a) Soybean-maize off-season double crop system in Brazil as affected by El Niño Southern Oscillation phases. Agric Syst 173:254–267. https://doi.org/10.1016/j.agsy.2019.03.012
Nóia Júnior RS, Sentelhas PC (2019b) Soybean-maize succession in Brazil: impacts of sowing dates on climate variability, yields and economic profitability. Eur J Agron 103:140–151. https://doi.org/10.1016/j.eja.2018.12.008
Noulas C, Tziouvalekas M, Karyotis T (2018) Zinc in soils, water and food crops. J Trace Elem Med Biol 49:252–260. https://doi.org/10.1016/j.jtemb.2018.02.009
Ojeda-Barrios DL, Perea-Portillo E, Hernández-Rodríguez OA, Martínez-Téllez J, Abadía J, Lombardini L (2014) Foliar fertilization with zinc in pecan trees. HortScience 49:562–566. https://doi.org/10.21273/HORTSCI.49.5.562
Pankievicz VCS, de Amaral FP, Santos KFDN, Agtuca B, Xu Y, Schueller MJ, Arisi ACM, Steffens MBR, de Souza EM, Pedrosa FO, Stacey G, Ferrieri RA (2015) Robust biological nitrogen fixation in a model grass-bacterial association. Plant J 81:907–919. https://doi.org/10.1111/tpj.12777
Pankievicz VCS, Irving TB, Maia LGS, Ané JM (2019) Are we there yet? The long walk towards the development of efficient symbiotic associations between nitrogen-fixing bacteria and non-leguminous crops. BMC Biol 17:99. https://doi.org/10.1186/s12915-019-0710-0
Penn CJ, Camberato J (2019) A critical review on soil chemical processes that control how soil pH affects phosphorus availability to plants. Agriculture 9:120. https://doi.org/10.3390/agriculture9060120
R Core Team R: a Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria (2015). https://www.R-project.org/
Raij B van, Cantarella H, Quaggio JA, Furlani AMC (1997) Liming and fertilization recommendations for the State of São Paulo. Campinas: Instituto Agronômico de Campinas, 285p. (Boletim técnico, 100) (in Portuguese).
Raij B van, Andrade JC, Cantarella H, Quaggio JA (2001) Chemical analysis for fertility evaluation of tropical soils. Campinas: IAC, 285p (in Portuguese).
Ram H, Rashid A, Zhang W, Duarte AP, Phattarakul N, Simunji S, Kalayci M, Freitas R, Rerkasem B, Bal RS, Mahmood K, Savasli E, Lungu O, Wang ZH, de Barros VLNP, Malik SS, Arisoy RZ, Guo JX, Sohu VS, Zou CQ, Cakmak I (2016) Biofortification of wheat, rice and common bean by applying foliar zinc fertilizer along with pesticides in seven countries. Plant Soil 403:389–401. https://doi.org/10.1007/s11104-016-2815-3
Rengel Z (2015) Availability of Mn, Zn and Fe in the rhizosphere. J Soil Sci Plant Nutr 15:397–409. https://doi.org/10.4067/S0718-95162015005000036
Sacristan D, Gonzalez-Guzman A, Barron V, Torrent J, Del Campillo MC (2019) Phosphorus-induced zinc deficiency in wheat pot-grown on noncalcareous and calcareous soils of different properties. Arch Agron Soil Sci 65:208–223. https://doi.org/10.1080/03650340.2018.1492714
Salvo LP, Ferrando L, Fernandéz-Scavino A, Salamone IEG (2018) Microorganisms reveal what plants do not: wheat growth and rhizosphere microbial communities after Azospirillum brasilense inoculation and nitrogen fertilization under field conditions. Plant Soil 424:405–417. https://doi.org/10.1007/s11104-017-3548-7
Sarma BK, Yadav SK, Singh DP, Singh HB (2012) Rhizobacteria mediated induced systemic tolerance in plants: prospects for abiotic stress management. In: Maheshwari DK (ed) Bacteria in agrobiology: stress management. Springer, Berlin, pp 225–238. https://doi.org/10.1007/978-3-642-23465-1_11
Sharma P, Jha AB, Dubey RS, Pessarakli M (2012) Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J Bot 2012:1–26. https://doi.org/10.1155/2012/217037
Shivay YS, Prasad R, Kumar S, Pal M (2015) Relative efficiency of zinc-coated urea and soil and foliar application of zinc sulphate on yield, nitrogen, phosphorus, potassium, zinc and iron biofortification in grains and uptake by basmati rice (Oryza sativa L.). J Agric Sci 7:161–173. https://doi.org/10.5539/jas.v7n2p161
Silva K, Silva EE, Farias ENC, Chaves JS, Albuquerque CNB, Cardoso C (2018) Agronomic efficiency of Bradyrhizobium preinoculation in association with chemical treatment of soybean seeds. Afr J Agric Res 13:726–732. https://doi.org/10.5897/AJAR2018.13016
Silva PCG, Tiritan CS, Echer FR, Cordeiro CFS, Rebonatti MD, Santos CH (2020) No-tillage and crop rotation increase crop yields and nitrogen stocks in sandy soils under agroclimatic risk. Field Crops Res 258:107947. https://doi.org/10.1016/j.fcr.2020.107947
Singh B, Natesan SKA, Singh BK, Usha K (2005) Improving zinc efficiency of cereals under zinc deficiency. Curr Sci 88:36–44
Soil Survey Staff (2014) Keys to soil taxonomy. Twelfh ed. USDA. Natural Resources Conservation Service, Washington, DC
Souza R, Ambrosini A, Passaglia LMP (2015) Plant growth-promoting bacteria as inoculants in agricultural soils. Gen Mol Biol 38:401–419. https://doi.org/10.1590/S1415-475738420150053
Teixeira PC, Donagemma GK, Fontana A, Teixeira WG (2017) Manual of soil analysis methods. Centro nacional de pesquisa de solos, Embrapa, Rio de Janeiro ((in Portuguese))
USDA. United States Department of Agriculture (2020) National agricultural statistics service. Crop production. http://www.nass.usda.gov/Publications/index.php. Accessed 20 Oct 2020
Wang YH, Zou CQ, Mirza Z, Li H, Zhang ZZ, Li DP, Xu CL, Zhou X, Shi XJ, Xie DT, He X, Zhang Y (2016) Cost of agronomic biofortification of wheat with zinc in China. Agron Sustain Dev 36:44–51. https://doi.org/10.1007/s13593-016-0382-x
Yang J, Kloepper JW, Ryu CM (2009) Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci 14:1–4. https://doi.org/10.1016/j.tplants.2008.10.004
Yang T, Siddique KHM, Liu K (2020) Cropping systems in agriculture and their impact on soil health—a review. Glob Ecol Conserv 23:e011118. https://doi.org/10.1016/j.gecco.2020.e01118
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421. https://doi.org/10.1111/j.1365-3180.1974.tb01084.x
Zaheer MS, Raza MAS, Saleem MF, Khan IH, Ahmad S, Iqbal R, Manevski K (2019) Investigating the effect of Azospirillum brasilense and Rhizobium pisi on agronomic traits of wheat (Triticum aestivum L.). Arch Agron Soil Sci 65:1554–1564. https://doi.org/10.1080/03650340.2019.1566954
Zeffa DM, Fantin LH, Santos OJAP, Oliveira ALM, Canteri MG, Scapim CA, Gonçalves LSA (2018) The influence of topdressing nitrogen on Azospirillum spp. inoculation in maize crops through meta-analysis. Bragantia 77:493–500. https://doi.org/10.1590/1678-4499.2017273
Zeffa DM, Perini LJ, Silva MB, de Sousa NV, Scapim CA, Oliveira ALM, Amaral Junior AT, Gonçalves LSA (2019) Azospirillum brasilense promotes increases in growth and nitrogen use efficiency of maize genotypes. PLoS ONE 14:e0215332. https://doi.org/10.1371/journal.pone.0215332
Zhang W, Liu K, Wang J, Shao X, Xu M, Li J, Wang X, Murphy DV (2015) Relative contribution of maize and external manure amendment to soil carbon sequestration in a long-term intensive maize cropping system. Sci Rep 5:10791. https://doi.org/10.1038/srep10791
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Galindo, F.S., Bellotte, J.L.M., Santini, J.M.K. et al. Zinc use efficiency of maize-wheat cropping after inoculation with Azospirillum brasilense. Nutr Cycl Agroecosyst 120, 205–221 (2021). https://doi.org/10.1007/s10705-021-10149-2
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DOI: https://doi.org/10.1007/s10705-021-10149-2