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Effect on intestinal microbiota, bioaccumulation, and oxidative stress of Carassius auratus gibelio under waterborne cadmium exposure

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Abstract

Cadmium (Cd) is a hazardous pollutant known to exert various toxic effects and other sublethal effects on aquatic organisms, and induce a variety of adverse effects on human health, and can be commonly found in environment. The aim of this study was to explore the effects of waterborne Cd exposure on the intestinal microbiota, and Cd accumulation and oxidative response in Carassius auratus gibelio (C. gibelio). The fish were exposed to waterborne Cd at 0, 1, 2, and 4 mg/L for 30 days. Waterborne Cd exposure resulted marked alterations in the composition of microbiota. At the genus level, Bacteroides, Aeromonas, Akkermansia, Acinetobacter, Chryseobacterium, Shinella, Cetobacterium and Bacillus were significantly changed in Cd groups. The results obtained indicate that Cd exposure significantly increased the bioaccumulation level of Cd and profoundly affected antioxidant enzyme including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), total antioxidant (T-AOC), malonaldehyde (MDA), and glutathione-S-transferase (GST). The present findings may provide a new framework for the role of gut microbiota in the response to environmental chemical contamination and in the pathogenesis of body disorders.

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Funding

The work was supported by the National Natural Sciences Foundational of China (no. 30972191) and the 948 Program from Ministry of Agriculture of China (no. 2014Z34).

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Author notes

  1. Nan Wang and Zhengyao Guo contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China

    Nan Wang, Zhengyao Guo, Yilin Zhang, Jia Liu, Yi Cheng, Lei Zhang & Yuehong Li

  2. Ministry of education laboratory of animal production and quality security, Jilin Agricultural University, Changchun, 130118, China

    Nan Wang, Zhengyao Guo, Yilin Zhang, Jia Liu, Yi Cheng, Lei Zhang & Yuehong Li

  3. Health Monitoring and Inspection Center of Jilin Province, Changchun, 130062, China

    Peijun Zhang

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  1. Nan Wang
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All authors participated in experiments, data processing, and paper writing. All authors agree to contribute to the journal without conflict of interest.

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Correspondence to Yuehong Li.

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This study was approved by the Ethics Committee of Jilin Agricultural University with ID no. 20121008. All authors read this guide carefully. All subjects signed their informed consents before participation.

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Wang, N., Guo, Z., Zhang, Y. et al. Effect on intestinal microbiota, bioaccumulation, and oxidative stress of Carassius auratus gibelio under waterborne cadmium exposure. Fish Physiol Biochem 46, 2299–2309 (2020). https://doi.org/10.1007/s10695-020-00870-0

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