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Dietary supplementation of selenium nanoparticles modulated systemic and mucosal immune status and stress resistance of red sea bream (Pagrus major)

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Abstract

Dietary supplementation of selenium nanoparticles (Se-NPs) at different levels (0, 0.5, 1, and 2 mg kg−1 diet) was evaluated to find out the effects on serum and skin immune responses as well as stress resistance in the red sea bream (Pagrus major). After 45 days of experimental trial, serum and mucosal immune responses were significantly high in fish fed 1 mg Se-NPs kg−1 diet (P < 0.05). In this group, alternative complement pathway, total serum protein, antioxidant activity of catalase enzyme, serum bactericidal activity, serum lysozyme activity, and amounts of skin mucus secretions as well as stress resistance against low salinity stress increased significantly, when compared to fish fed Se-NP-free diet (P < 0.05). Furthermore, fish fed Se-NPs at 2 mg kg−1 diet exhibited higher alternative complement pathway, total serum protein, mucus lysozyme activity, serum and mucus peroxidases, amount of mucus secreted, and tolerance against low salinity stress than the fish fed Se-NP-free diet (P < 0.05). Interestingly, the nitro blue tetrazolium activity in all groups fed with diets supplemented with Se-NPs are significantly higher than Se-NP-free diet (P < 0.05). The present results demonstrate that the dietary supplementation with Se-NPs (mainly from 1 to 2 mg kg−1 level) could be useful for maintaining the overall health status of red sea bream.

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Authors and Affiliations

  1. Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima, 890-0056, Japan

    Mahmoud A. O. Dawood, Shunsuke Koshio & Amr I. Zaineldin

  2. Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

    Mahmoud A. O. Dawood

  3. Animal Health Research Institute(AHRI-DOKI), Cairo, Egypt

    Amr I. Zaineldin

  4. Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand

    Hien Van Doan

  5. Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

    Eman Moustafa Moustafa

  6. Department of Pharmacology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt

    Mohamed M. Abdel-Daim

  7. Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100, Murcia, Spain

    M. Angeles Esteban

  8. Aquaculture Division Fish Nutrition Research Laboratory, National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt

    Mohamed S. Hassaan

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  1. Mahmoud A. O. Dawood
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Dawood, M.A.O., Koshio, S., Zaineldin, A.I. et al. Dietary supplementation of selenium nanoparticles modulated systemic and mucosal immune status and stress resistance of red sea bream (Pagrus major). Fish Physiol Biochem 45, 219–230 (2019). https://doi.org/10.1007/s10695-018-0556-3

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