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Ontogeny and kinetics of carnitine palmitoyltransferase I in hepatopancreas and skeletal muscle of grass carp (Ctenopharyngodon idella)

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Abstract

The ontogeny and kinetics of carnitine palmitoyltransferase I (CPT I) were investigated in hepatopancreas and muscle throughout four developmental stages (newly hatched larvae, 1-month-old juvenile, 3-month-old, and 6-month-old, respectively) of grass carp Ctenopharyngodon idella. In hepatopancreas, the maximal velocity (V max) significantly increased from hatching to 1-month-old grass carp and then gradually declined at 6-month-old grass carp. In muscle, CPT I activity was the highest at 1-month-old grass carp, nearly twofold higher than that at hatching (P < 0.05). The Michaelis constant (K m) value was also the highest for 1-month-old in both tested tissues. Carnitine concentrations (FC, AC and TC) were the lowest for 3-month-old grass carp and remained relatively constant in both tissues from fish under the other developmental stages. The FC concentration in hepatopancreas and muscle at four developmental stages were less than the respective K m, indicating that grass carp required supplemental carnitine in their food to ensure that CPT I activity was not constrained by carnitine availability.

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Abbreviations

AC:

Acyl carnitine

TC:

Total carnitine

FC:

Free carnitine

ANOVA:

One-way analysis of variance

CPT:

Carnitine palmitoyltransferase

DTNB:

5, 5′-dithio-bis-(2-nitrobenzoic acid)

K m :

Michaelis–Menten constants

MS 222:

Tricaine methanesulfonate

SEM:

Standard error of mean

V max :

Maximal reaction velocity

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Acknowledgments

This work was funded by ‘973’ project, China (Grant No. 2009CB118706), and the Special Fund for Agro-Scientific Research in the Public Interest of China (Grant No. 201003020).

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Author notes

  1. Cai-Xia Liu

    Present address: Bureau of Aquatic Products of Xiantao City, Xiantao, 433000, People’s Republic of China

  2. Jia-Lang Zheng

    Present address: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, People’s Republic of China

Authors and Affiliations

  1. Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Wei Hu, Zhi Luo, Cai-Xia Liu & Jia-Lang Zheng

  2. Freshwater Aquaculture Collaborative Innovative Centre of Hubei Province, Wuhan, 430070, People’s Republic of China

    Wei Hu, Zhi Luo, Cai-Xia Liu & Jia-Lang Zheng

  3. College of Fisheries, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Kang-Sen Mai

Authors
  1. Wei Hu
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  2. Zhi Luo
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  3. Kang-Sen Mai
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  4. Cai-Xia Liu
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Correspondence to Zhi Luo.

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Hu, W., Luo, Z., Mai, KS. et al. Ontogeny and kinetics of carnitine palmitoyltransferase I in hepatopancreas and skeletal muscle of grass carp (Ctenopharyngodon idella). Fish Physiol Biochem 41, 1393–1401 (2015). https://doi.org/10.1007/s10695-015-0094-1

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