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The male sterility of bread wheat line XN291S was resulted from the deletion of a large segment at the end of 6BS

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Abstract

The sterile lines used in the production of two-line hybrid wheat in China are mainly photoperiod-thermo sensitive male sterile (PTSMS), and it is of great significance to study the sterile features and mechanisms of novel sterile lines for wheat hybrid breeding. XN291S, a spontaneous mutant of XN291, was sterile under long-day/high-temperature and fertile under short-day/low-temperature. In this study, its male sterility was mainly controlled by the high temperature, and occurred in the binucleate stage. The sterility gene at high temperature was a major recessive XNms-6B gene located on 6BS by genetic analysis and molecular marker genetic mapping, and the large fragment deletion at the end of 6BS was proved by genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH) and further genomic resequencing. This fragment corresponded to the 0–88.3 Mb physical interval of the Chinese Spring Reference Genome (IWGSCv1.0), and contained 1077 high-confidence genes. The results of multi-omics association analysis showed that starch and sucrose metabolism, phenylpropane biosynthesis, linoleic acid metabolism, and secondary metabolite biosynthesis were the key pathways, and the deleted genes involved in heat stress response and anther development directly or indirectly resulted in the male sterility of XN291S. This study will contribute to further study of XNms-6B, and lay a foundation for the application of this sterile line in hybrid wheat breeding.

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Data availability statement

The raw transcriptomic data of XN291S and XN291 presented in the study are deposited in the NCBI Sequence Read Archive repository, accession number PRJNA810250. The raw proteomics data of XN291S and XN291 are deposited in iProX (https://www.iprox.cn), project ID: IPX0004184000.

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Acknowledgements

The authors are grateful to Prof. Puhui Dong (Henan University of Science and Technology) for his valuable materials and suggestion. We would like to thank Prof. Fangpu Han (Institute of Genetics and Development, Chinese Academy of Sciences) for his support for FISH and GISH.

Funding

This study was financially supported by the National Key Research and Development Program of China (Grant No. 2022YFD1200803 and 2016YFD0101602).

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Authors and Affiliations

  1. Key Laboratory of Crop Heterosis and Utilization (MOE) and Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China

    Minghu Zhang, Jiazheng Yu, Qijun Xie, Shaozhe Wen, Jinghui Li, Chan Bi, Chaojie Xie, Zhongfu Ni, Rongqi Liang & Mingshan You

  2. State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, 071001, China

    Minghu Zhang

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  1. Minghu Zhang
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Contributions

MY conceived the project and revised the manuscript. RL conceived the project and wrote the manuscript. MZ performed the experiments and wrote the manuscript. JY analyzed the omics data. QX, SW, JL and BC performed some survey on the field trials. CX and ZN supported greenhouse and gave the valuable suggestions. All authors contributed to the article and approved the submitted version.

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Correspondence to Rongqi Liang or Mingshan You.

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Zhang, M., Yu, J., Xie, Q. et al. The male sterility of bread wheat line XN291S was resulted from the deletion of a large segment at the end of 6BS. Euphytica 219, 62 (2023). https://doi.org/10.1007/s10681-023-03191-4

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