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Genetic mapping of tolerance to iron deficiency chlorosis in peanut (Arachis hypogaea L.)

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Abstract

Iron deficiency chlorosis (IDC) under calcareous and alkaline soils is a significant abiotic stress affecting the growth and yield of peanut. In this study, the genomic regions governing IDC tolerance were mapped using a recombinant inbred line (RIL) population derived from TMV 2 (susceptible to IDC) and TMV 2-NLM (tolerant to IDC), which was phenotyped during the rainy seasons of 2019 and 2020 in the iron-deficient calcareous plots. The best linear unbiased prediction (BLUP) values for IDC tolerance traits like visual chlorotic rating (VCR), and SPAD chlorophyll meter reading (SCMR) were used for QTL analysis along with a genetic map carrying 700 GBS-derived SNP, AhTE and SSR markers. In total, 11 and 12 main-effect QTLs were identified for VCR and SCMR, respectively. Among them three QTLs were major with the phenotypic variance explained (PVE) of 10.3–34.4% for VCR, and two QTL were major for SCMR with PVE of 11.5–11.7%. A region (159.3–178.3 cM) on chromosome Ah13 carrying two QTLs (one each for VCR and SCMR) was consistent with the previous report. A SNP marker, Ah14_138037990 identified from single marker analysis for VCR was located in the intronic region of the gene Arahy.QA0C1, which is important for protein-binding. Overall, this study identified new QTLs and also validated QTL for IDC tolerance. These genomic resources could be useful for genomics-assisted breeding of peanut for IDC tolerance.

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Acknowledgements

Authors acknowledge the support of Department of Genetics and Plant Breeding, College of Agriculture, Vijayapur for phenotyping and Institute of Agri-Biotechnology (IABT), UAS, Dharwad and Centre of Excellence in Genomics & Systems Biology (CEG&SB), ICRISAT, Patancheru, India for SNP genotyping/sequencing work. The financial support received from ICAR-NASF (New Delhi) is also acknowledged.

Funding

The funding support for genotyping-by-sequencing (GBS) performed in this study was received from National Agricultural Science Fund (NASF) of Indian Council of AgriculturalResearch, India.

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Authors and Affiliations

  1. Department of Biotechnology, University of Agricultural Sciences, Dharwad, 580 005, India

    Ankur D. Tayade, Mangesh P. Jadhav & Ramesh S. Bhat

  2. AICRP On Groundnut, Main Agriculture Research Station (MARS), University of Agricultural Sciences, Dharwad, 580 005, India

    Babu N. Motagi

  3. College of Agriculture, University of Agricultural Sciences, Dharwad, Vijayapur, 586 101, India

    Anjum S. Nadaf

  4. Department of Crop Physiology, University of Agricultural Sciences, Dharwad, 580 005, India

    Rajshekar V. Koti

  5. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, 502 324, India

    Sunil S. Gangurde, Vinay Sharma, Rajeev K. Varshney & Manish K. Pandey

  6. State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, Food Futures Institute, Murdoch University, Murdoch, 6150, Australia

    Rajeev K. Varshney

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  1. Ankur D. Tayade
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  2. Babu N. Motagi
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  3. Mangesh P. Jadhav
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  9. Manish K. Pandey
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  10. Ramesh S. Bhat
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Correspondence to Babu N. Motagi or Ramesh S. Bhat.

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Tayade, A.D., Motagi, B.N., Jadhav, M.P. et al. Genetic mapping of tolerance to iron deficiency chlorosis in peanut (Arachis hypogaea L.). Euphytica 218, 46 (2022). https://doi.org/10.1007/s10681-022-02996-z

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