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Transformation of Campanula by wild type Agrobacterium rhizogenes

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Abstract

Compact growth is an important quality criterion in horticulture. Many Campanula species and cultivars exhibit elongated growth which is suppressed by chemical retardation and cultural practice during production to accommodate to the consumer’s desire. The production of compact plants via transformation with wild type Agrobacterium rhizogenes is an approach with great potential to produce plants that are non-GMO. Efficient transformation and regeneration procedures vary widely among both plant genera and species. Here we present a transformation protocol for Campanula. Hairy roots were produced on 26–90% of the petioles that were used for transformation of C. portenschlagiana (Cp), a C. takesimana × C. punctata hybrid (Chybr) and C. glomerata (Cg). Isolated hairy roots grew autonomously and vigorously without added hormones. The Cg hairy roots produced chlorophyll and generated plantlets in response to treatments with cytokinin (42 µM 2iP) and auxin (0.67 µM NAA). In contrast, regeneration attempts of transformed Cp and Chybr roots lead neither to the production of chlorophyll nor to the regeneration of shoots. Agropine A. rhizogenes strains integrate split T-DNA in TL- and TR-DNA fragments into the plant genome. In this study, regenerated plants of Cg did not contain TR-DNA, indicating that a selective pressure against this T-DNA fragment may exist in Campanula.

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Acknowledgements

This project was funded by the innovation consortium “Innovative Plants” through the Danish Agency for Science, Technology and Innovation. A. rhizogenes strain A4 was kindly provided by Dr. David Tepfer, Laboratoire de Biologie de la Rhizosphère, INRA, Versailles, Cédex, France. Gartneriet PKM A/S is greatly acknowledged for providing plant material. We thank Christian Hald Madsen and Kell Kristiansen for interesting discussions.

Authors’ contribution

JNH and SVW developed the transformation procedure. JNH, HL and RM designed the experiments. JNH and UBL performed the experiments and analysed the data. JNH, HL and UBL wrote the manuscript. All authors approved the final manuscript.

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Authors and Affiliations

  1. Crop Sciences Section, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Højbakkegård Allé 9-13, 2630, Taastrup, Denmark

    Josefine Nymark Hegelund, Uffe Bjerre Lauridsen, Sabá Victoria Wallström, Renate Müller & Henrik Lütken

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  1. Josefine Nymark Hegelund
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  2. Uffe Bjerre Lauridsen
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  3. Sabá Victoria Wallström
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  4. Renate Müller
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  5. Henrik Lütken
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Correspondence to Josefine Nymark Hegelund.

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Hegelund, J.N., Lauridsen, U.B., Wallström, S.V. et al. Transformation of Campanula by wild type Agrobacterium rhizogenes . Euphytica 213, 51 (2017). https://doi.org/10.1007/s10681-017-1845-0

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