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Association mapping and favorable allele mining for node of first fruiting/sympodial branch and its height in Upland cotton (Gossypium hirsutum L.)

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Abstract

Node of first fruiting/sympodial branch (NFFB) and its height (HNFFB) are two important indicators to measure cotton (Gossypium spp.) early maturity. This study performed an association mapping using the mixed linear model (MLM) procedure for NFFB and HNFFB based on 172 Upland cotton (Gossypium hirsutum L.) cultivars and 331 polymorphic SSR markers. The gene diversity index of 331 markers ranged from 0.0387 to 0.7799 with a mean value of 0.4002 and the polymorphism information content ranged from 0.0379 to 0.7473 with a mean value of 0.3375. A total of 18 markers associated with NFFB were detected in at least two environments, and the range of explained phenotypic variation by markers in different environments was 2.28–11.36 % with a mean value of 5.20 %. A total of 19 markers associated with HNFFB were detected in at least two environments, and the range of explained phenotypic variation by markers in different environments was 2.83–16.96 % with a mean value of 7.92 %. These markers detected in multiple environments are of good stability and can be used for marker-assisted selection (MAS) of target traits. In addition, favorable alleles for NFFB and HNFFB were mined. These favorable alleles and their representative cultivars are expected to be used for breeding practices. This study provides theoretical basis for the further analysis of genetic basis of NFFB and HNFFB, as well as the use of MAS for cotton early maturity.

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Abbreviations

NFFB:

Node of first fruiting/sympodial branch

HNFFB:

Height of node of first fruiting/sympodial branch

SSR:

Simple sequence repeat

QTL:

Quantitative trait locus/loci

LD:

Linkage disequilibrium

MLM:

Mixed linear model

MAS:

Marker-assisted selection

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Acknowledgments

This research was supported by National Natural Science Foundation of China (31371677), Cotton Industrial Technology System Program of Henan Province (S2013-07, Z2013-07-01), Key Project of Science and Technology of Henan Province (14A210006), and Program of University Young Core Instructor of Henan Province (2013GGJS-136).

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Authors and Affiliations

  1. Collaborative Innovation Center of Modern Biological Breeding, Henan Province/Cotton Research Institute, Henan Institute of Science and Technology, Xinxiang, 453003, Henan, China

    Chengqi Li, Jinbao Zhang, Genhai Hu, Yuanzhi Fu & Qinglian Wang

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  1. Chengqi Li
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  2. Jinbao Zhang
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  3. Genhai Hu
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  4. Yuanzhi Fu
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  5. Qinglian Wang
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Correspondence to Chengqi Li or Qinglian Wang.

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Li, C., Zhang, J., Hu, G. et al. Association mapping and favorable allele mining for node of first fruiting/sympodial branch and its height in Upland cotton (Gossypium hirsutum L.). Euphytica 210, 57–68 (2016). https://doi.org/10.1007/s10681-016-1697-z

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