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Development of a breeding method for durable resistance to angular leaf spot in common bean

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Abstract

Durable resistance, conditioned by minor resistance genes, has been described as more reliable in managing angular leaf spot (ALS) disease in the long term. The aim of this study was to develop a breeding method for durable ALS resistance in common bean and use it to accumulate minor genes into single genotypes. Four genotypes with intermediate resistance to ALS were selected from an initial 182 genotypes and used to generate a double cross segregating population. The F1 was planted in a nethouse and inoculated with a mixture of races of the ALS pathogen. The genotypes that showed intermediate resistance to ALS were subsequently selected from the F1 to the F3 segregating populations. In the F3 generation resistant plants were selected and the F4 plants were evaluated, together with the parents and several market class varieties. Data were collected on ALS disease severity, seed yield, days to physiological maturity, seed size, seed colour and growth habit. Ten F4 advanced lines with enhanced levels of resistance (disease score 1.9–3.2) were selected. These lines had improved resistance when compared to their parents (disease score 4.6–4.8) and market class varieties (disease score 5.4–8.2), which confirmed breeding progress for resistance to ALS. Simultaneous selection was done for seed yield, seed size, farmer preferred seed type and growth habit. The results of the study have shown that it is possible to develop ALS resistant common bean lines through the double cross method, using a mixture of ALS races.

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References

  • Asensio SMMC, Asensio C, Singh SP (2006) Gamete selection for resistance to common and halo bacterial blights in dry bean intergene pool populations. Crop Sci 46:131–135

    Article  Google Scholar 

  • Damasceno e Silva KJ, Souza EA, sartorato A, Frier CS (2008) Pathogenic variability of isolates of Pseudocercospora griseola, the cause of common bean angular leaf spot, and its implications for resistance breeding. J Phytopathol 156:602–606

    Article  Google Scholar 

  • Danial D, Parlevliet J, Almekinders C, Thiele G (2007) Farmers’ participation and breeding for durable disease resistance in the Andean region. Euphytica 153:385–396

    Article  Google Scholar 

  • Gichangi A, Maobe SN, Karanja D, Getabu A, Macharia CN, Ogecha JO, Nyang’au MK, Basweti E, Kitonga L (2012) Assessment of production and marketing of climbing beans by smallholder farmers in Nyanza region, Kenya. World J Agric Sci 8:293–302

    Google Scholar 

  • Gonza´ lez AM, Rodin’o AP, M Santalla, Ron AMD (2009) Genetics of intra-gene pool and inter-gene pool hybridization for seed traits in common bean (Phaseolus vulgaris L.) germplasm from Europe. Field Crop Res 112:66–76

    Article  Google Scholar 

  • Johnson R (1981) Durable resistance: definition of, genetic control and attainment in plant breeding. Phytopathology 71:567–568

    Article  Google Scholar 

  • Katungi E, Farrow A, Chianu J, Sperling I, Beebe S (2009) Common bean in Eastern and Southern Africa: a situation and outlook analysis. CIAT, Cali

    Google Scholar 

  • Mahuku GS, Jara C, Cuasquer JB, Castellanos G (2002) Genetic variability within Phaeoisariopsis griseola from Central America and its implications for resistance breeding. Plant Pathol 51:594–604

    Article  Google Scholar 

  • Mahuku GS, Iglesias AM, Jara C (2009) Genetics of angular leaf spot resistance in the Andean common bean accession G5686 and identification of markers linked to the resistance genes. Euphytica 167:381–396

    Article  CAS  Google Scholar 

  • Mahuku GS, Henrı´quez MA, Montoya C, Jara C, Teran H, Beebe S (2011) Inheritance and development of molecular markers linked to angular leaf spot resistance genes in the common bean accession G10909. Mol Breed 28:57–71

    Article  Google Scholar 

  • Ojwang’ PPO, Melis R, Songa JM, Githiri M, Bett C (2009) Participatory plant breeding approach for host plant resistance to bean fly in common bean under semi-arid Kenya conditions. Euphytica 170:383–393

    Article  Google Scholar 

  • Parlevliet JE (1983) Can horizontal resistance be recognized in the presence of vertical resistance in plants exposed to a mixture of pathogen races? Phytopathology 73:379

    Article  Google Scholar 

  • Parlevliet JE (1985) Resistance of the non-race-specific type. In: Roelfs AP, Bushnell WR (eds) The cereal rusts, vol II. Academic Press, New York, pp 501–525

    Google Scholar 

  • Parlevliet JE (1995) Genetic and breeding aspects of durable resistance of crops to pathogens. Afr Crop Sci J 3:1–13

    Google Scholar 

  • Parlevliet JE, van Ommeren A (1988) Accumulation of partial resistance in barley to barley leaf rust and powdery mildew through recurrent selection against susceptibility. Euphytica 37:261–274

    Article  Google Scholar 

  • Pastor-Corrales MA, Jara C, Singh SP (1998) Pathogenic variation in, source of, and breeding for resistance to Phaeoisariopsis griseola causing angular leaf spot in common bean. Euphytica 103:161–171

    Article  Google Scholar 

  • Payne RW, Murray DA, Harding SA, Baird DB, Soutar DM (2009) GenStat for Windows (12th Edition) Introduction. VSN International, Hemel Hempstead

    Google Scholar 

  • Robinson RA (1980) New concepts in breeding for disease resistance. Annu Rev of Phytopathol 18:189–210

    Article  Google Scholar 

  • Robinson RA (1987) Plant pathosystems. Springer-Verlag, Berlin

    Google Scholar 

  • Singh SP (1994) Gamete selection for simultaneous improvement of multiple traits in common bean. Crop Sci 34:352–355

    Article  Google Scholar 

  • Singh SP (2001) Broadening the genetic base of common bean cultivars: a review. Crop Sci 41:1659–1675

    Article  Google Scholar 

  • Singh SP, Cardona C, Morales FJ, Pastor-Corrales MA, Voysest O (1998) Gamete selection for upright Carioca bean with resistance to five diseases and a leafhopper. Crop Sci 38:666–672

    Article  Google Scholar 

  • Singh SP, Teran H, Lema M, Davis MF, Hayes R, Robinson C (2008) Breeding for slow darkening, high yielding, broadly adapted dry bean pinto ‘Kimberly’ and ‘Shoshone’. J Plant Registration 2:181–186

    Google Scholar 

  • Teran H, Singh SP (2009) Gamete selection for improving physiological resistance to white mold in common bean. Euphytica 167:271–289

    Article  Google Scholar 

  • Teran H, Jara C, Mahuku G, Beebe S, Singh SP (2013) Simultaneous selection for resistance to five bacterial, fungal and viral diseases in three Andean × Middle American inter-gene pool common bean populations. Euphytica 189:283–292

    Article  CAS  Google Scholar 

  • Van der Plank JE (1968) Disease resistance in plants. Academic Press, New York

    Google Scholar 

  • Van Schoonhoven A, Pastor-Corrales MA (eds) (1987) Standard system for the evaluation of bean germplasm. CIAT, Cali

    Google Scholar 

  • Wagara IN, Mwang’ombe AW, Kimenju JW, Buruchara RA (2005) Virulence, variability and physiological races of angular leaf spot pathogen Phaeoisariopsis griseola in Kenya. Afr Plant Prot 11:23–31

    Google Scholar 

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Acknowledgments

This research was carried out by the main author while undertaking PhD studies at the University of KwaZulu–Natal’s, African Centre for Crop Improvement (ACCI). Funds for the study were provided by the Rockefeller Foundation and Bill & Melinda Gates Foundation (BMGF) through the Alliance for a Green Revolution in Africa (AGRA). Commendable institutional support was provided by the Horticultural Crops Development Authority (HCDA, now the Agriculture, Fisheries and Food Authority, Horticultural Crops Directorate) and University of Nairobi (UoN), College of Agriculture and Veterinary Sciences.

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Authors and Affiliations

  1. Agriculture, Fisheries and Food Authority, Horticulture Crops Directorate, P.O. Box 42601-00100, Nairobi, Kenya

    Beatrice N. Ng’ayu-Wanjau

  2. African Centre for Crop Improvement, University of KwaZulu-Natal, Scottsville 3209, Private Bag X01, Pietermaritzburg, KwaZulu-Natal, South Africa

    Rob Melis & Julia Sibiya

  3. Horticulture Department, Faculty of Agriculture, Jomo Kenyatta University of Agriculture and Technology, P.O. Box 62000-00200, Nairobi, Kenya

    Mwangi Githiri

  4. Department of Crop Science, Faculty of Agriculture and Veterinary Sciences, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya

    Paul M. Kimani

Authors
  1. Beatrice N. Ng’ayu-Wanjau
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  2. Rob Melis
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  3. Mwangi Githiri
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  4. Julia Sibiya
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  5. Paul M. Kimani
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Correspondence to Beatrice N. Ng’ayu-Wanjau.

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Ng’ayu-Wanjau, B.N., Melis, R., Githiri, M. et al. Development of a breeding method for durable resistance to angular leaf spot in common bean. Euphytica 208, 91–100 (2016). https://doi.org/10.1007/s10681-015-1582-1

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  • DOI: https://doi.org/10.1007/s10681-015-1582-1

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