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Response of 21 common beans of diverse origins to two strains of the common bacterial blight pathogen, Xanthomonas campestris pv. phaseoli

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Abstract

Common bacterial blight (CBB) is a severe disease of common bean and the use of resistant cultivars is the most effective control. The objectives were to determine (i) the most appropriate leaf type for CBB evaluation, (ii) the aggressiveness of two bacterial strains, (iii) the presence or absence of SAP6, BC420, and SU91 resistance QTL linked markers, and (iv) the most resistant genotypes. The CBB response in the primary and trifoliolate leaves of 21 genotypes of diverse origins and two checks was evaluated in two greenhouses. Mean trifoliolate leaf score (4.8) was higher than the primary leaf (2.5). The strain Xcp25 (3.2 primary, 5.4 trifoliolate) was more aggressive than ARX8AC (1.7 primary, 4.2 trifoliolate). Andean ‘Montcalm’ with SAP6 QTL was intermediate (6.0) to ARX8AC and susceptible (8.3) to Xcp25 in the trifoliolate leaf. New Andean RCS52-2, RCS53-3, and RCS63-5B with BC420 and SU91, and 08SH840 with SAP6 and SU91 QTL were intermediate (3.5–6.2) to both strains. But, Middle American VAX 3, VAX 4, and VAX 6 with SAP6 and SU91 QTL were resistant (2.3–2.5) to ARX8AC and intermediate to Xcp25 (3.4–6.5) in the trifoliolate leaf. Further efforts are required to pyramid higher levels of resistance from across Phaseolus species and introgressed in Andean common bean.

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Abbreviations

CBB:

Common bacterial blight

QTL:

Quantitative trait locus or loci

SCAR:

Sequence characterized amplified region

Xcp :

Xanthomonas campestris pv. phaseoli

Xff :

Xanthomonas fuscans sbsp. fuscans sp. nov

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Acknowledgments

We thank Drs. Phillip Miklas for supplying seed of USDK-CBB-15 and USWK-CBB-17, Tim Porch for seed of 08SH840 and CXR 1, and M. Carmen-Asensio-Manzanera-S. for seed of the RCS Andean common bean breeding lines. We also thank Drs. Phillip Miklas and Howard Schwartz for their valuable comments and edits. The greenhouse and laboratory support from the Idaho Agricultural Experiment Station at Kimberly to carry out this research is gratefully acknowledged.

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  1. Plant, Soil and Entomological Sciences Department, Kimberly Research & Extension Center, University of Idaho, 3793 North 3600 East, Kimberly, ID, 83341-5076, USA

    Diego M. Viteri & Shree P. Singh

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  1. Diego M. Viteri
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  2. Shree P. Singh
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Correspondence to Diego M. Viteri.

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Viteri, D.M., Singh, S.P. Response of 21 common beans of diverse origins to two strains of the common bacterial blight pathogen, Xanthomonas campestris pv. phaseoli . Euphytica 200, 379–388 (2014). https://doi.org/10.1007/s10681-014-1161-x

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