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Molecular and morphological characterisation of Aphelenchoides paraxui n. sp. (Nematoda: Aphelenchoididae) isolated from Quercus brantii in western Iran

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Abstract

Aphelenchoides paraxui n. sp. is described and illustrated from bark samples of an oak tree (Quercus brantii L.) in Kermanshah province, western Iran. The new species is characterized by body length of 500–660 μm (females) and 630–665 μm (males), lip region set off from body contour, lateral fields with four lines, and total stylet 8–9 μm long with small basal swellings. The excretory pore is located ca one body diam. Posterior to metacorpus valve. The spicules are relatively large (29–33 μm in dorsal limb) with apex and rostrum rounded and well developed and the end of the dorsal limb clearly curved ventrad like a hook. The female tail is conical, the terminus having a complicated step-like projection, usually with many tiny nodular protuberances. Male tail bearing six (2 + 2 + 2) caudal papillae and a well-developed mucro. The new species belongs to the Group 2 category of Aphelenchoides species sensu Shahina (1996) in which eight known species among Group 2–4 sensu Shahina namely: A. arcticus, A. asteromucronatus, A. blastophthorus, A. lichenicola, A. saprophilus, A. seiachicus, A. silvester and A. xui, are the most closed species. Molecular analyses of the partial small subunit rDNA gene (SSU), D2/D3 expansion segments of the large subunit rDNA gene (LSU) and internal transcribed spacer (ITS) revealed this as a new species and supported the morphological results.

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Notes

  1. The specific epithet was chosen because the new species is morphologically similar to A. xui.

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Acknowledgements

This research was financially supported by the University of Tehran, Iran. The Iranian authors thank Mr. Shahab Esmaeili (University of Tehran) for technical help.

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  1. Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    Mehrab Esmaeili & Ramin Heydari

  2. Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Yiwu Fang & Hongmei Li

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  1. Mehrab Esmaeili
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Correspondence to Ramin Heydari.

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Esmaeili, M., Heydari, R., Fang, Y. et al. Molecular and morphological characterisation of Aphelenchoides paraxui n. sp. (Nematoda: Aphelenchoididae) isolated from Quercus brantii in western Iran. Eur J Plant Pathol 149, 625–637 (2017). https://doi.org/10.1007/s10658-017-1212-y

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