Abstract
One new and three known species of the genus Xiphinema from the rhizosphere of fruit trees and rose shrubs in East Azarbaijan province, Iran, are presented based on the morphological, morphometric and molecular characters. The new species is distinguished by its 2.0-2.1 mm long body, relatively flattened lip region with 8.7-10.0 µm width, set off from body contour by a deep constriction, odontostyle 82.5-88.0 µm long, V = 52-54, reproductive system didelphic-amphidelphic with symbiotic bacteria in the reflexed ovaries, tail conoid, dorsally convex with rounded to slightly subdigitate tip (42.0-43.5 µm long, c = 61-65, c′ = 1.6-1.8), and males unknown. The new species, X. babaii sp. n., looks very close to X. californicum, and is regarded as its cryptic species, being separated from it using some morphological differences. Their separation was further corroborated using molecular data. Three known species belonging to the Xiphinema americanum group namely X. primum, X. pachtaicum and X. simile were also collected during present study, and new data were provided for them. Xiphinema simile is a new record for the Iran's nematode fauna. Molecular phylogenetic studies using partial sequences of 28S rRNA gene D2-D3 fragments were performed, and the phylogenetic relationships of the new species were discussed.
Similar content being viewed by others
References
Archidona-Yuste, A., Navas-Cortés, J. A., Cantalapiedra-Navarrete, C., Palomares-Rius, J. E., & Castillo, P. (2016). Cryptic diversity and species delimitation in the Xiphinema americanum-group complex (Nematoda: Longidoridae) as inferred from morphometrics and molecular markers. Zoological Journal of the Linnean Society, 176(2), 231-265. https://doi.org/10.1111/zoj.12316
Bazgir, E., Naghavi, A., & Zolfaghari, Z. (2020). Three species of Xiphinema americanum-group complex (Nematoda: Longidoridae), from Lorestan province, Iran. Helminthologia, 57(4), 394-401. https://doi.org/https://doi.org/10.2478/helm2020-0036
De Grisse, A. T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires. Mededelingen Rijksfakulteit Landbouwwetenschappen, Gent, 34, 351-369.
Fadaei, A. A., Coomans, A., & Kheiri, A. (2003). Three species of the Xiphinema americanum lineage (Nematoda: Longidoridae) from Iran. Nematology, 5(3), 453-61.
Getaneh, G., Bert, W., & Decraemer, W. (2015). First report, morphological and molecular characterization of Xiphinema elongatum and X. pachtaicum (Nematoda, Longidoridae) from Ethiopia. ZooKeys, 489, 1-13.
Jahanshahi Afshar, F., Pedram, P., & Mobasseri, M. (2021). Description of Xiphinema persicum n. sp. (Nematoda: Longidoridae), a X. americanum-group species from Iran. European Journal of Plant Pathology, 159, 783-797. https://doi.org/https://doi.org/10.1007/s10658-021-02201-2
Jahanshahi Afshar, F., Shahryari, F., Mokhtassi-Bidgoli, A., Castillo, P., Fouladvand, Z. M., & Pedram, M. (2020). Occurrence of Xiphinema santos Lamberti, Lemos, Agostinelli & D’Addabo 1993 (Nematoda: Longidoridae), a X. americanum-group member in Iran. European Journal of Plant Pathology, 157, 281-291. https://doi.org/https://doi.org/10.1007/s10658-020-01980-4
Kirjanova, E. S. (1951). Soil nematodes found in cotton fields and in virgin soil of Golodnaya Steppe (Uzbekistan). [In Russian]. Trudy Zoologicheskogo Instituta Akademiya Nauk USSR, 9, 625-657.
Lamberti, F., Choleva, B., & Agostinelli, A. (1983). Longidoridae from Bulgaria (Nematoda, Dorylaimida) with description of three new species of Longidorus and two new species of Xiphinema. Nematologia Mediterranea, 11, 49-72.
Lamberti, F., Hockland, S., Agostinelli, A., Moens, M., & Brown, D. J. F. (2004). The Xiphinema americanum group. III. Keys to species identification. Nematologia Mediterranea, 32, 53-56.
Lamberti, F., Molinari, S., Moens, M., & Brown, D. J. F. (2000). The Xiphinema americanum group. I. Putative species, their geographical occurrence and distribution, and regional polytomous identification keys for the group. Russian Journal of Nematology, 8(1), 65-84.
Lamberti, F., Molinari, S., Moens, M., & Brown, D. J. F. (2002). The Xiphinema americanum-group. II. Morphometric relationships. Russian Journal of Nematology, 10(2), 99-112.
Lazarova, S., Peneva, V., & Kumari, S. (2016). Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae). ZooKeys, 574, 1-42.
Mobasseri, M., Hutchinson, M. C., Jahanshahi Afshar, F., & Pedram, M. (2019). New evidence of nematode endosymbiont bacteria coevolution based on one new and one known dagger nematode species of Xiphinema americanum-group (Nematoda, Longidoridae). PLoS ONE, 14(6), e0217506. https://doi.org/https://doi.org/10.1371/journal.pone.0217506
Mojtahedi, H., Sturhan, D., Akhiani, A., & Barooti, S. (1980). Xiphinema species in Iranian vineyards. Nematologia Mediterranea, 8, 170-95.
Mortazavi, P. & Pedram, M. (2020). Description of Aphelenchoides hamospiculatus n. sp. (Aphelenchoidea: Aphelenchoididae) from Golestan province, north Iran. Nematology, 23(2), 201-213. https://doi.org/https://doi.org/10.1163/15685411-bja10038
Nunn, G. B. (1992). Nematode molecular evolution: an investigation of evolutionary patterns among nematodes based upon DNA sequences. Ph.D. Dissertation, University of Nottingham, Nottingham, UK.
Nylander, J. A. A. (2004). MrModeltest v2. Program distributed by the author. Uppsala University, Sweden, Evolutionary Biology Centre.
Pedram, M. (2018). Nematodes of the family Longidoridae. In: Ghaderi, R., Kashi, L. & Karegar, A. (Eds). Plant-parasitic nematodes in Iran. Marjae Elm in collaboration with the Iranian Society of Nematology, pp. 627-667. (In Persian).
Pedram, M., Pourjam, E., Namjou, S., Atighi M. R., Cantalapiedra-Navarrete, C., Liébanas, G., Palomares-Rius, J. E. & Castillo, P. (2012). Molecular and morphological characterisation of Paralongidorus iranicus n. sp. and Paralongidorus bikanerensis (Lal & Mathur, 1987) Siddiqi, Baujard & Mounport, 1993 (Nematoda: Longidoridae) from Iran. Nematology, 14, 427-443. https://doi.org/https://doi.org/10.1163/156854111X610272
Pedram, M., Soleymanzadeh, M., Pourjam, E. & Mobasseri, M. (2018). Observations on Malenchus geraerti n. sp. (Rhabditida: Tylenchidae), a morphological and molecular phylogenetic study. Zootaxa, 4369 (3): 406-418. https://doi.org/https://doi.org/10.11646/zootaxa.4369.3.6
Ronquist, F., & Huelsenbeck, J. P. (2003). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 19, 1572-1574. https://doi.org/https://doi.org/10.1093/bioinformatics/17.8.754
Siddiqi, M.R. (2000) Tylenchida: parasites of plants and insects (2nd Ed.). CABI, New York, 833 pp.
Soleymanzadeh, M., Pedram, M., Pourjam, E., & Álvarez-Ortega, S. (2016). Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family. Nematology, 18, 987-998. https://doi.org/https://doi.org/10.1163/15685411-00003010
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725-2729. https://doi.org/https://doi.org/10.1093/molbev/mst197
Taylor, C. E. & Brown, D. J. F. (1997). Nematode vectors of plant viruses. Wallingford, UK, CAB International.
Whitehead, A. G., & Hemming, J.R. (1965). A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55, 25-38.
Ye, W., Szalanski, A. L. and Robbins, R. T. (2004). Phylogenetic relationships and genetic variation in Longidorus and Xiphinema species (Nematoda: Longidoridae) using ITS1 sequences of nuclear ribosomal DNA. Journal of Nematology, 36(1), p.14.
Funding
This research was financially supported by University of Tabriz, Tabriz, Iran.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest. The authors have no relevant financial or non-financial interests to disclose.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Naghavi, A., Niknam, G. & Vazifeh, N. A new species of Xiphinema americanum group (Nematoda: Longidoridae) from Iran, with additional data on three known species. Syst Parasitol 99, 545–555 (2022). https://doi.org/10.1007/s11230-022-10044-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11230-022-10044-6