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Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine

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Abstract

Grapevine fanleaf virus (GFLV) is the causal agent of grapevine degeneration disease, which causes progressive decline of infected vines and lowers the yield. The most important strategy to prevent the spread of GFLV is using healthy planting material and elimination of diseased plants. GFLV titre is known to be variable during the season; therefore we elaborated a spatio–temporal study of its distribution in different grapevine organs during the season. Our research showed that young leaves have high virus titre during the whole vegetative period, while mature leaves, tendrils and flower/berry clusters only at the beginning of the vegetative period. However seeds retained high virus titre after berries colouring. Phloem scrapings were shown to contain lower virus titres during the vegetative period, with the increase outside and at the beginning of the vegetative period. In flower/berry clusters, mature leaves and in tendrils, GFLV titre decreased significantly over the vegetative period. Semi-quantitative results obtained by ELISA were confirmed by qPCR. Additionally, different GFLV titres were shown in five different cultivars and different combinations of mixed infections with other grapevine viruses influenced the GFLV titre differently. Finally, correlation between the magnitude of symptoms’ appearance and GFLV titres was analysed.

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Acknowledgments

Operation part financed by the European Union, European Social Fund. Operation implemented in the framework of the Operational Programme for Human Resources Development for the Period 2007–2013, Priority axis 1: Promoting entrepreneurship and adaptability, Main type of activity 1.1.: Experts and researchers for competitive enterprises.

We thank all vineyards owners for kindly providing materials from their vineyards.

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Authors and Affiliations

  1. Department of Biotechnology and Systems Biology, National Institute of Biology (NIB), Večna Pot 111, 1000, Ljubljana, Slovenia

    Anastazija Jež Krebelj, Urška Čepin, Maja Ravnikar & Maruša Pompe Novak

  2. School for Viticulture and Enology, University of Nova Gorica (UNG), Glavni trg 8, 5271, Vipava, Slovenia

    Anastazija Jež Krebelj, Maja Ravnikar & Maruša Pompe Novak

  3. Regional Development Agency of Northern Primorska Ltd. Nova Gorica (RRA SP), Trg Edvarda Kardelja 3, 5000, Nova Gorica, Slovenia

    Anastazija Jež Krebelj

  4. Biosistemika research and development Ltd., Tehnološki park 24, 1000, Ljubljana, Slovenia

    Urška Čepin

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  1. Anastazija Jež Krebelj
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  2. Urška Čepin
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  3. Maja Ravnikar
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  4. Maruša Pompe Novak
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Correspondence to Anastazija Jež Krebelj.

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Krebelj, A.J., Čepin, U., Ravnikar, M. et al. Spatio-temporal distribution of Grapevine fanleaf virus (GFLV) in grapevine. Eur J Plant Pathol 142, 159–171 (2015). https://doi.org/10.1007/s10658-015-0600-4

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  • DOI: https://doi.org/10.1007/s10658-015-0600-4

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