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Monitoring by real-time PCR of three water-borne zoosporic Pythium species in potted flower and tomato greenhouses under hydroponic culture systems

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Abstract

The high-temperature-tolerant Pythium species P. aphanidermatum, P. helicoides, and P. myriotylum cause serious diseases in many crops under hydroponic culture systems in Japan. Control of the diseases is difficult because these zoosporic pathogens spread quickly. In this study, a real-time PCR method was developed for monitoring the spread of zoospores of the three pathogens. Specific primers and TaqMan probes were established using the internal transcribed spacer regions of the rDNA. Specificity was confirmed using known isolates of each species and closely related non-target species. The sensitivity of DNA detection was 10 f. for each pathogen. 10 f. DNA corresponded to 4 P. aphanidermatum, 3 P. myriotylum, and 4 P. helicoides zoospores, respectively. Therefore, this real-time PCR method was used to evaluate and monitor zoospores in the nutrient solutions of ebb-and-flow irrigation systems for potted flower production and closed hydroponic culture systems for tomato production. The results indicated that the pathogens were present in the hydroponic culture systems throughout the year, and spread before disease occurrence.

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Acknowledgment

This research was funded by the development of diagnostic manual of high-temperature tolerant Pythium species in hydroponic culture system project for application in promoting new policy of Japanese Ministry of Agriculture Forestry and Fisheries. We thank Dr. M. D. Coffey, and Dr. S. Uematsu for providing isolates of Phytophthora species.

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Author notes

  1. Mingzhu Li

    Present address: College of Life Sciences, Shaanxi Normal University, Xi’an, 710062, China

Authors and Affiliations

  1. River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Mingzhu Li, Yasushi Ishiguro, Kayoko Otsubo & Koji Kageyama

  2. Division of Regenerative Technology, Mie Prefecture Agricultural Research Center, Matsusaka, Mie, 515-2316, Japan

    Hirofumi Suzuki & Tomoko Tsuji

  3. Agri-environmental Division, Aichi Agricultural Research Center, Nagakute, Aichi, 480-1193, Japan

    Noriyuki Miyake & Hirofumi Nagai

  4. Life Science Research Center, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Haruhisa Suga

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  1. Mingzhu Li
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  2. Yasushi Ishiguro
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  3. Kayoko Otsubo
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  4. Hirofumi Suzuki
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Correspondence to Yasushi Ishiguro.

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Li, M., Ishiguro, Y., Otsubo, K. et al. Monitoring by real-time PCR of three water-borne zoosporic Pythium species in potted flower and tomato greenhouses under hydroponic culture systems. Eur J Plant Pathol 140, 229–242 (2014). https://doi.org/10.1007/s10658-014-0456-z

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  • DOI: https://doi.org/10.1007/s10658-014-0456-z

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