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Characterization of a Fusarium poae world-wide collection by using molecular markers

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Abstract

Fusarium poae has been considered as a minor species among those that cause Fusarium Head Blight (FHB) disease but in recent years several researchers have documented a high frequency of occurrence of this species. In this study, a total of 173 F. poae isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated by using inter simple sequence repeats (ISSR) and amplified fragment length polymorphism (AFLP) to evaluate genetic variability within F. poae and to amplify MAT idiomorphs as a possible mechanism that could explain part of the variability found in this species. The molecular analysis obtained from both molecular markers showed a high intraspecific variability. However, a partial clustering between F. poae isolates and their geographic origin was obtained by ISSR markers while AFLP showed isolates from different geographic locations distributed throughout the dendrogram. Moreover, ISSR grouped all the F. poae isolates into a different cluster from the F. langsethiae and F. sporotrichioides isolates used as outgroups compared with the dendrogram obtained using AFLP markers. Analysis of molecular variance (AMOVA) indicated a high genetic variability in the F. poae collection, with most of the genetic variability resulting from differences within, rather than between, American and European populations by using both molecular markers. Regarding MAT idiomorphs, for most F. poae isolates both MAT-1 and MAT-2 were present from each isolate.

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References

  • Agarwal, M., Shrivastava, N., & Padh, H. (2008). Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Reports, 27, 617–631.

    Article  CAS  PubMed  Google Scholar 

  • Akinsanmi, O. A., Backhouse, D., Simpfendorfer, S., & Chakraborty, S. (2008). Mycelial compatibility reactions of Australian Fusarium graminearum and F. pseudograminearum isolates compared with AFLP groupings. Plant Pathology, 57, 251–261.

    Article  Google Scholar 

  • Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. P. (1990). Basic local alignment search tool. Journal of Molecular Evolution, 215, 403–410.

    CAS  Google Scholar 

  • Alvarez, C. L., Somma, S., Proctor, R. H., Stea, G., Mulé, G., Logrieco, A. F., et al. (2011). Genetic diversity in Fusarium graminearum from a major wheat-producing region of Argentina. Toxins, 3, 1294–1309.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Astolfi, P., Reynoso, M. M., Ramirez, M. L., Chulze, S. N., Alves, T. C. A., Tessmann, D. J., et al. (2011). Genetic populations structure and trichothecene genotypes of Fusarium graminearum isolated from wheat in southern Brazil. Plant Pathology, 61, 289–295.

    Article  Google Scholar 

  • Audenaert, K., Van Broeck, R., Bekaert, B., De Witte, F., Heremans, B., Messens, K., et al. (2009). Fusarium head blight (FHB) in Flanders: population diversity, inter-species associations and DON contamination in commercial Winter wheat varieties. European Journal of Plant Pathology, 125, 445–458.

    Article  Google Scholar 

  • Bassam, B. J., Caetano-Anollés, G., & Gresshoff, P. M. (1991). Fast and sensitive silver staining of DNA in polyacrilamide gels. Analytical Biochemistry, 196, 80–83.

    Article  CAS  PubMed  Google Scholar 

  • Cepni, E., Tunali, B., & Gürel, F. (2012). Genetic diversity and mating types of Fusarium culmorum and Fusarium graminearum originating from different agro-ecological regions in Turkey. Journal of Basic Microbiology, 52, 1–9.

    Article  Google Scholar 

  • Chandra Nayaka, S., Wulff, E. G., Udayashankar, A. C., Nandini, B. P., Niranjana, S. R., Mortensen, C. N., et al. (2011). Prospects of molecular markers in Fusarium species diversity. Applied Microbiology and Biotechnology, 90, 1625–1639.

    Article  CAS  PubMed  Google Scholar 

  • Conde-Ferráez, L. (2007). El locus mat (mating-type) de los ascomicetos: su evolución, estructura y regulación. Revista Iberoamericana de Micología, 24, 95–99.

    Article  PubMed  Google Scholar 

  • Dinolfo, M. I., Stenglein, S. A., Moreno, M. V., Nicholson, P., Jennings, P., & Salerno, G. L. (2010). ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England. European Journal of Plant Pathology, 127, 483–491.

    Article  CAS  Google Scholar 

  • Irzykowska, L., Bocianowski, J., & Baturo-Ciesniewska, A. (2013). Association of mating-type with mycelium growth rate and genetic variability of Fusarium culmorum. Central European Journal of Biology, 8, 701–711.

    Article  CAS  Google Scholar 

  • Kerényi, Z., Táborhegyi, É., Pomázi, A., & Hornok, L. (1997). Variability amongst strains of Fusarium poae assessed by vegetative compatibility and RAPD polymorphism. Plant Pathology, 46, 882–889.

    Article  Google Scholar 

  • Kerényi, Z., Moretti, A., Waalwijk, C., Oláh, B., & Hornok, L. (2004). Mating type sequences in asexually reproducing Fusarium species. Applied and Environmental Microbiology, 70, 4419–4423.

    Article  PubMed Central  PubMed  Google Scholar 

  • Kristler, H. C., & Miao, V. P. M. (1992). New models of genetic change in filamentous fungi. Annual Review of Phytopathology, 30, 131–152.

    Article  Google Scholar 

  • Leslie, J. F., & Summerell, B. A. (2006). The Fusarium laboratory manual. Ames: Blackwell Publishing.

    Book  Google Scholar 

  • Leslie, J. F., Anderson, L. L., Bowden, R. L., & Lee, Y.-W. (2007). Inter- and intra-specific genetic variation in Fusarium. International Journal of Food Microbiology, 119, 25–32.

    Article  CAS  PubMed  Google Scholar 

  • Lindblad, M., Gidlund, A., Sulyok, M., Borjesson, T., Krska, R., Olsen, M., et al. (2013). Deoxynivalenol and other selected Fusarium toxins in Swedish wheat—ocurrence and correlation to specific Fusarium species. International Journal of Food Microbiology. doi:10.1016/j.ijfoodmicro.2013.07.002.

    Google Scholar 

  • Manzo-Sánchez, G., James-Kay, A., Ortiz-Vázquez, E., & Simpson-Williamson, J. (2007). Desarrollo de mapas genéticos y físicos de hongos fitopatógenos: aplicaciones y perspectivas. Revista Mexicana de Fitopatología, 25, 54–65.

    Google Scholar 

  • Mishra, P. K., Fox, R. T., & Culham, A. (2003). Inter-simple sequence repeat and aggressiveness analyses revealed high genetic diversity, recombination and long-range dispersal in Fusarium culmorum. Annals of Applied Biology, 143, 291–301.

    Article  CAS  Google Scholar 

  • Mishra, P. K., Tewari, J. P., Clear, R. M., & Turkington, T. K. (2004). Molecular genetic variation and geographical structuring in Fusarium graminearum. Annals of Applied Biology, 145, 299–307.

    Article  CAS  Google Scholar 

  • Nei, M. (1987). Molecular evolutionary genetics. New York: Columbia University Press. 512 pp.

    Google Scholar 

  • Nicholson, P., Chandler, E., Draeger, R. C., Gosman, N. E., Simpson, D. R., Thomsett, M., et al. (2003). Molecular tools to study spidemiology and toxicology of fusarium head blight of cereals. European Journal of Plant Pathology, 109, 691–703.

    Article  CAS  Google Scholar 

  • Ouellet, T., & Seifert, K. A. (1993). Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification. Phytopathology, 83, 1003–1007.

    Article  CAS  Google Scholar 

  • Parry, D. W., & Nicholson, P. (1996). Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathology, 45, 383–391.

    Article  CAS  Google Scholar 

  • Qu, B., Li, H. P., Zhang, J. B., Xu, Y. B., Huang, T., Wu, A. B., et al. (2008). Geographic distribution and genetic diversity of Fusarium graminearum and F. asiaticum on wheat spikes throughout China. Plant Pathology, 57, 15–24.

    Article  CAS  Google Scholar 

  • Rohlf, F. I. (1998). NTSYSpc. Numerical taxonomy and multivariate analysis system version 2.0. Applied biostatistics. New York: Exeter Software.

    Google Scholar 

  • Schneider, S., Roessli, D., & Excoffier, L. (2000). Arlequin. Version 2.0: A software for populations genetic data analysis. Switzerland: University of Geneva.

    Google Scholar 

  • Sneath, P. H., & Sokal, R. R. (1973). Numeral taxonomy. San Francisco: Freeman.

    Google Scholar 

  • Somma, S., Alvarez, C., Ricci, V., Ferracane, L., Ritieni, A., Logrieco, A., et al. (2010). Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy. Food Additives and Contaminants, 27, 729–737.

    Article  CAS  PubMed  Google Scholar 

  • Stenglein, S. A., & Balatti, P. A. (2006). Genetic diversity of Phaeoisariopsis griseola in Argentina as revealed by pathogenic and molecular markers. Physiological and Molecular Plant Pathology, 68, 158–167.

    Article  CAS  Google Scholar 

  • Stenglein, S. A., Dinolfo, M. I., Bongiorno, F., & Moreno, M. V. (2012). Response of wheat (Triticum spp.) and barley (Hordeum vulgare) to Fusarium poae. Agrociencia, 46, 299–306.

    Google Scholar 

  • Su, H., Wang, L., Liu, L., Chi, X., & Zhang, Y. (2008). Use of inter-simple sequence repeat markers to develop strain-specific SCAR markers for Flammulina velutipes. Journal of Applied Genetics, 49, 233–235.

    Article  PubMed  Google Scholar 

  • Vogelgsang, S., Enkerli, J., Jenny, E., Roffler, S., & Widmer, F. (2010). Characterization of Fusarium poae microsatellite markers on strains from Switzerland and other countries. Journal of Phytopathology, 159, 197–200.

    Article  Google Scholar 

  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., et al. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23, 4407–4414.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Yun, S.-H., Arie, T., Kaneko, I., Yoder, O. C., & Turgeon, B. G. (2000). Molecular organization of mating type loci in heterothallic, homothallic, and asexual Gibberella/Fusarium species. Fungal Genetics and Biology, 31, 7–20.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. Paul Nicholson for critical comments and suggestions on improving this manuscript. This research was supported by FONCYT PICT 110/2008 and 030/2011, CONICET and UNCPBA.

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Authors and Affiliations

  1. Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-INBIOTEC-CICBA, Facultad de Agronomía de Azul, UNCPBA, Av. República de Italia # 780, 7300, Azul, Buenos Aires, Argentina

    María I. Dinolfo, Eliana Castañares & Sebastián A. Stenglein

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    María I. Dinolfo, Eliana Castañares & Sebastián A. Stenglein

  3. Cátedra de Microbiología. Facultad de Agronomía de Azul, UNCPBA, Av. de Italia # 780, 7300, Azul, Buenos Aires, Argentina

    Sebastián A. Stenglein

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  1. María I. Dinolfo
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  2. Eliana Castañares
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  3. Sebastián A. Stenglein
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Correspondence to Sebastián A. Stenglein.

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Dinolfo, M.I., Castañares, E. & Stenglein, S.A. Characterization of a Fusarium poae world-wide collection by using molecular markers. Eur J Plant Pathol 140, 119–132 (2014). https://doi.org/10.1007/s10658-014-0448-z

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