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Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs

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Abstract

Imazethapyr, a post-emergent herbicide used in worldwide soybean and corn crops, induces genetic and biochemical alterations in aquatic vertebrates. This study examined the relationship between biomarkers at different organization levels and imazethapyr real-life route exposure in Boana pulchella adults. Frogs were exposed to imazethapyr-based formulation Pivot® H (10.59%) at concentrations representing possible acute routes: field runoff (S1:10 mg.L−1), exposure after direct foliar application (S2:100 mg.L−1) and during direct foliar application (S3:1000 mg.L−1). Post-exposure, endpoints levels were evaluated: organism alterations, biochemical activities and cytogenetic assays. Forty-eight hours post-exposure, antioxidant enzymes decrease, micronuclei induction and DNA damage were observed in all scenarios, while cholinesterase activity increase and body condition reduction were observed in frog-exposed to S3. Ninety-six hours post-exposure, frogs showed glutathione-S-transferase inhibition in S1, micronuclei induction in S2 and S3, and DNA-damage increase in S3. Herbicides routes of exposures in real-life could indicate that authorized applications have a risk to amphibian populations.

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Abbreviations

AChE:

acetylcholinesterase

BL:

blebbed nucleus

BN:

binucleated cell

CAT:

catalase

GST:

glutathione-S-transferase

IMZT:

imazethapyr

LB:

lobed nucleus

MNs:

micronuclei

NT:

notched nucleus

PCA:

principal component analysis.

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Funding

This study was funded by Doctoral Internal Scholarships Granting Institution: National Commission for Scientific and Technical Research (CONICET). Grants File No. 11/N817 and 11/N847, Research Project at the National University of La Plata: “Empleo de herramientas bioanalíticas de respuesta temprana a la exposición de contaminantes emergentes en sistemas in vivo e in vitro. Facultad de Ciencias Naturales y Museo. Project I + D: 11/N746”, the project from Agencia Nacional de Promoción Científica y Tecnológica (FONCYT): PICT 2015-3137 “Desarrollo y aplicación de herramientas de diagnóstico de contaminación ambiental utilizando anuros autóctonos como indicadores”, and to the Research Project 2019-PE-E2-I054 Funded by INTA.

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Authors and Affiliations

  1. Centro de Investigaciones del Medio Ambiente (CIM), CONICET. Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 y 115, La Plata, B1900, Argentina

    J. M. Pérez-Iglesias & G. S. Natale

  2. Cátedra de Citología, Facultad de Ciencias Naturales y Museo. CONICET, Universidad Nacional de La Plata, Calle 64 N°3 esq. 120, Lab. 17, La Plata, B1900, Argentina

    J. M. Pérez-Iglesias & M. L. Larramendy

  3. Instituto de Química de San Luis, CONICET. Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco 917, San Luis, D5700, Argentina

    J. M. Pérez-Iglesias

  4. Instituto de Recursos Biológicos, Centro de Investigaciones de Recursos Naturales (CIRN), Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham (B1686), Buenos Aires, Argentina

    J. C. Brodeur

Authors
  1. J. M. Pérez-Iglesias
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  2. G. S. Natale
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  3. J. C. Brodeur
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  4. M. L. Larramendy
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Pérez-Iglesias, J.M. (JMPI), Brodeur, J.C. (JCB), Natale, G.S. (GSN) and Larramendy, M.L (MLL). The first draft of the manuscript was written by JMPI and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. Conceptualization: JMPI, JCB, GSN and MLL; Methodology: JMPI, JCB, GSN and MLL; Formal analysis and investigation: JMPI, JCB, and MLL; Writing – original draft preparation: JMPI; Writing – review and editing: JCB and MLL; Funding acquisition and Resources: JCB, GSN and MLL; Supervision: JCB and MLL.

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Correspondence to J. M. Pérez-Iglesias.

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Pérez-Iglesias, J.M., Natale, G.S., Brodeur, J.C. et al. Realistic scenarios of pesticide exposure alters multiple biomarkers in BOANA PULCHELLA (ANURA) Adult Frogs. Ecotoxicology 32, 309–320 (2023). https://doi.org/10.1007/s10646-023-02639-6

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