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Synergistic influence of Vetiveria zizanioides and selected rhizospheric microbial strains on remediation of endosulfan contaminated soil

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Abstract

Application of endosulfan tolerant rhizospheric bacterial strain isolated from pesticide contaminated area, Ghaziabad in combination with V. zizanioides for the remediation of endosulfan is described herein. The dissipation of endosulfan from soil was considerably enhanced in the presence of bacterial strain and Vetiveria zizanioides together when compared to the dissipation in presence of either of them alone. Four strains— EAG-EC-12 (M1), EAG-EC-13(M2), EAG-EC-14(M3) and EAG-EC-15(M4) are used for this purpose. V. zizanioides was grown in garden soil spiked with 1500 µg g–1 of endosulfan and inoculated with 100 ml of microbial culture of above motioned strains. Effect of microbial inoculation on plant growth, endosulfan uptake and endosulfan removal efficiency were analyzed. The microbial inoculation significantly enhances the growth of test plant and endosulfan dissipation from soil (p < 0.05). The addition of bacterial strain M1, M2, M3 and M4 in treated pots showed enhanced root length by 13, 33 35, 20.2 and 4.3 %, above ground plant length by 16.38, 35.56, 24.92 and 9.8 % and biomass by 33.69, 49.63, 39.24 and 17.09 % respectively when compared with endosulfan treated plants. After 135 days of exposure, a decline in endosulfan concentration by 59.12, 64.56, 62.69 and 56.39 % was obtained in the spiked soil inoculated with bacterial strains M1, M2, M3 and M4 respectively whereas, decrease in endosulfan concentration by 72.78, 85.25, 76.91 and 65.44 % in the vegetative spiked soil inoculated with same strains was observed during same exposure period. After 135 days of growth period, enhanced removal of endosulfan from experimental soil by 13.66, 20.69, 14.22 and 9.05 % was found in vegetative experiment inoculated with same strains when compared with non vegetative experiment. Result of the study showed that use of toletant plant and tolerant bacterial strains could be the better strategy for the remediation of endosulfan contaminated soil.

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References

  • Abhilash PC, Srivastava S, Srivastava P, Singh B, Jafri A, Singh N (2011) Influence of rhizospheric microbial inoculation and tolerant plant species on the rhizoremediation of lindane. Environ Exp Bot 74:127–130

    Article  CAS  Google Scholar 

  • Agency of Toxic Substances and Disease Registry, Toxicological Profile for Endosulfan, 2000

  • Ahamed GJ, Wang MM, Zhou YH, Xia XJ, Mao WH, Shi K (2012) The growth, photosynthesis and antioxidant defense responses of five vegetable crops to phenanthrene stress. Ecotoxicol Environ Saf 80:132–139

    Article  Google Scholar 

  • Anastassiades M, Lehotay S, Štajnbaher D, Schenck F (2003) Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solidphase extraction for the determination of pesticide residues in produce. J. AOAC Int 86(22):412–431

    CAS  Google Scholar 

  • Becerra-Castro C, Kidd PS, Rodríguez-Garrido B, Monterroso C, Santos-Ucha P, Prieto-Fernández A (2013) Phytoremediation of hexachlorocyclohexane (HCH)-contaminated soils using Cytisus striatus and bacterial inoculants in soils with distinct organic matter content. Environ Pollut 178:202–210

    Article  CAS  Google Scholar 

  • Bhadauria BS, Mathur VB, Kaul R (2012) Monitoring of organochlorine pesticides in and around Keoladeo National Park, Bharatpur, Rajasthan, India. Environ Monit Assess 184:5295–5300

    Article  Google Scholar 

  • Chaudhry Q, Blom-Zandstra M, Gupta S, Joner EJ (2005) Utilizing the synergy between plants and rhizosphere microorganisms to enhance breakdown of organic pollutants in the environment. Environ Sci Pollut Res 12:34–48

    Article  CAS  Google Scholar 

  • Datta R, Das P, Smith S, Pravin Punamiya P, Ramanathan DM, Reddy R, Sarkar D (2013) Phytoremediation potential of vetiver grass [Chrysopogon zizanioides (L.)] for tetracycline. Int J Phytoremediation 15(4):343–351

    Article  CAS  Google Scholar 

  • Devi N, Chakraborty P, Shihua Q, Zhang G (2013) Selected organochlorine pesticides (OCPs) in surface soils from three major states from the north eastern part of India. Environ Monit Assess 185(8):6667–6676

    Article  CAS  Google Scholar 

  • Epelde L, Mijangos I, Becerril JM, Garbisu C (2009) Soil microbial community as bioindicator of the recovery of soil functioning derived from metal phytoextraction with sorghum. Soil Biol Biochem 41(9):1788–1794

    Article  CAS  Google Scholar 

  • Glick BR, Stearns JC (2011) Making phytoremediation work better: maximizing a plant’s growth potential in the midst of adversity. Int J Phytorem 13(1):4–16

    Article  Google Scholar 

  • Glick BR, Cheng Z, Czarny J, Duan J (2007) Promotion of plant growth by ACC deaminase-producing soil bacteria. Eur J Plant Pathol 119:329–339

    Article  CAS  Google Scholar 

  • Huang XD, EI-Alawi Y, Penrose DM, Glick BR, Greenberg BM (2005) A multi-process phytoremediation system for removal of polyaromatic hydrocarbons from contaminated soils. Environ Pollut 130:465–476

    Article  Google Scholar 

  • Hussain S, Arshad M, Saleem M, Khalid A (2007) Biodegradation of alpha and beta endosulfan by soil bacteria. Biodegradation 18:731–740

    Article  CAS  Google Scholar 

  • Ibrahim SI, Lateef MFA, Khalifa HMS, Monem AEA (2013) Phytoremediation of atrazine-contaminated soil using Zea mays (maize). Annals of Agriculture Science 58(1):69–75

    Article  Google Scholar 

  • Kataoka R, Takagi K (2013) Biodegradability and biodegradation pathways of endosulfan and endosulfan sulfate. Appl Microbiol Biotechnol 97:3285–3292

    Article  CAS  Google Scholar 

  • Katayama A, Matsumura F (1993) Degradation of organochlorine pesticides particularly endosulfan by Trichoderm Harzianum. Environ Toxicol Chem 12:1059–1065

    Article  CAS  Google Scholar 

  • Kidd PS, Prieto-Fernandez A, Monterroso C (2008) Rhizospheric microbial community and hexachlorocyclohexane degradative potential in contrasting plant species. Plant Soil 32:233–247

    Article  Google Scholar 

  • Kingsley MT, Fredrickson JK, Metting FB, Seidler RJ (1994) Environmental restoration using plant-microbe bioaugmentation. In: Hinchee RE, Leeson A, Semprini L, Ong SK (eds) Bioremediation of chlorinated and polyaromatic hydrocarbon compounds. Lewis Publishers, Boca Raton, FL, pp 287–292

    Google Scholar 

  • Korade DL, Fulekar MH (2009) Rhizosphere remediation of chlorpyrifos in mycorrhizospheric soil using ryegrass. J Hazard Mater 172(2–3):1344–1350

    Article  CAS  Google Scholar 

  • Kuiper I, Bloemberg GV, Lugtenberg BJJ (2001) Selection of a plant-bacterium pair as a novel tool for rhizostimulation of polycyclic aromatic hydrocarbon-degrading bacteria. Mol Plant Microbe Interact 14:1197–1205

    Article  CAS  Google Scholar 

  • Kuiper I, Legendijk EL, Bloemberg GV, Lugtenberg BJJ (2004) Rhizoremediation: a beneficial plant-microbe interaction. Mol Plant-Microbe Interact 17:6–15

    Article  CAS  Google Scholar 

  • Kullman SW, Matsumura F (1996) Metabolic pathways utilized by Phanerochaete chrysosporium for degradation of the cyclodiene pesticide endosulfan. Appl Environ Microbiol 62(2):593–600

    CAS  Google Scholar 

  • Kumar M, Lakshmi VC, Khanna S (2008) Biodegradation and bioremediation of endosulfan contaminated soil. Bioresour Technol 99:3116–3122

    Article  CAS  Google Scholar 

  • Li Z, Xiao H, Cheng S, Zhang L, Xie X, Wu Z (2014) A comparison on the phytoremediation ability of triazophos by different macrophytes. J Environ Sci 26(2):315–322

    Article  CAS  Google Scholar 

  • Martens M (1976) Degradation of [8,9–14C]endosulfan by soil microorganisms. Appl Environ Microbiol 6:853–858

    Google Scholar 

  • Mukherjee I, Kumar A (2012) Phytoextraction of endosulfan a remediation technique. Bull Environ Contam Toxicol 88:250–254

    Article  CAS  Google Scholar 

  • Nelson JA, Morgan JA, LeCain DA, Mosier AR, Milchunas DG, Parton BA (2004) Elevated CO2 increases soil moisture and enhances plant water relations in a long term field study in semi-arid shortgrass steppe of Colorado. Plant Soil 259:169–179

    Article  CAS  Google Scholar 

  • Odukkathil G, Vasudevan N (2013) Enhanced biodegradation of endosulfan and its major metabolite endosulfate by a biosurfactant producing bacterium. J Environ Sci Health Part B 48:462–469

    Article  CAS  Google Scholar 

  • Ontanon OM, Gonzalez PS, Ambrosio LF, Paisio CE, Agostini E (2014) Rhizoremediation of phenol and chromium by the synergistic combination of a native bacterial strain and Brassica napushairy roots. Int Biodeterior Biodegradation 88:192–198

    Article  CAS  Google Scholar 

  • Panno MTD, Morelli IS, Engelen B, Berthe-corti L (2005) Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation. FEMS Microbiol Ecol 53:305–316

    Article  Google Scholar 

  • Ramirez-Sandoval M, Melchor-Partidaa GN, Muniz-Hernandez S, Giron-Perez MI, Rojas-Garcia AE, Medina-Diaz IM, Robledo-Marenco ML, Velazquez Fernandez JB (2011) Phytoremediatery effect and growth of two species of Ocimum in endosulfan polluted soil. J Hazard Mater 192:388–392

    CAS  Google Scholar 

  • Rani R, Juwarkar A (2012) Biodegradation of phorate in soil and rhizosphere of Brassica juncea (L.) (Indian Mustard) by a microbial consortium. Int Biodeterior Biodegradation 71:36–42

    Article  CAS  Google Scholar 

  • Shahsavari E, Adetutu EM, Taha M, Ball AS (2015) Rhizoremediation of phenanthrene and pyrene contaminated soil using wheat. J Environ Manage 155:171–176

    Article  CAS  Google Scholar 

  • Sicilliano SD, Germida JJ, Banks MK, Greer CW (2003) Changes in microbial community composition and function during polyaromatic hydrocarbom phytoremediation field trial. Appl Environ Microbiol 69:483–489

    Article  Google Scholar 

  • Silambarasan S, Abraham J (2014) Halophilic bacterium JSA4 in biomineralization of endosulfan and its metabolites isolated from Gossypium herbaceum rhizosphere soil. J Taiwan Inst Chem E 45(4):1748–1756

    Article  CAS  Google Scholar 

  • Singh N, Megharaj M, Kookana R, Naidu R, Sethunathan N (2004) Atrazine and simazine degradation in Pennnisetum rhizospher. Chemosphere 56:257–263

    Article  CAS  Google Scholar 

  • Singh V, Singh N (2014) Uptake and accumulation of endosulfan isomers and its metabolite endosulfan sulfate in naturally growing plants of contaminated area. Ecotoxicol Environ Saf 104:189–193

    Article  CAS  Google Scholar 

  • US-EPA (2002) Reregistration Eligibility Decision for Endosulfan. EPA 738-R-02-013. Pollution, Pesticides and Toxic Substances (7508C), United States Environmental Protection Agency. http://www.epa.gov/oppsrrd1/REDs/endosulfan_red.pdf

  • Vancura V, Hovadik A (1965) Root exudates of plants II. Composition of root exudates of some vegetables. Plant Soil 22:21–32

    Article  CAS  Google Scholar 

  • Warsaw AL, Fernandez RT, Kort DR, Cregg BM, Rowe B, Vandervoort C (2012) Remediation of metalaxyl, trifluralin, and nitrate from nursery runoff using container-grown woody ornamentals and phytoremediation areas. Ecol Eng 47:254–263

    Article  Google Scholar 

  • Xie HN, Gao FW, Tan W, Wang SG (2011) A short term study on the interaction of bacteria, fungi and endosulfan in soil microcosm. Sci Total Environ 412–413:375–379

    Article  Google Scholar 

Download references

Acknowledgments

Authors are grateful to the Director CSIR- National Botanical Research Institute (NBRI) for providing necessary facilities. Authors also wish to thank the Department of Science and Technology (DST) for financial support as DST-WOS-A project.

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  1. Eco Auditing Group, National Botanical Research Institute (Council of Scientific & Industrial Research), Rana Pratap Marg, Lucknow, U.P, 226001, India

    Vandana Singh, Pratiksha Singh & Nandita Singh

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  1. Vandana Singh
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  2. Pratiksha Singh
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Correspondence to Vandana Singh or Nandita Singh.

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Singh, V., Singh, P. & Singh, N. Synergistic influence of Vetiveria zizanioides and selected rhizospheric microbial strains on remediation of endosulfan contaminated soil. Ecotoxicology 25, 1327–1337 (2016). https://doi.org/10.1007/s10646-016-1685-z

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