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CYP1B1-AS1 Delays the Malignant Progression of Colorectal Cancer by Binding with NOP58

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Abstract

Background

Colorectal cancer (CRC) is a prevalent type of gastrointestinal cancer, and its poor prognosis is mainly attributed to the occurrence of invasion and metastasis. CYP1B1-AS1, as non-coding RNA, plays an important role in tumorigenesis and progression. However, the mechanism by which CYP1B1-AS1 acts in CRC is not yet understood.

Aims

The objective of this study was to investigate how CYP1B1-AS1 contributes to the development of CRC, and provide a base for CRC diagnosis and treatment.

Methods

RT-qPCR was used to detect the expression level of CYP1B1-AS1 in CRC and adjacent tissues. CCK-8, Edu, scratch healing, and transwell experiments were used to detect the changes of proliferation, migration, and invasion ability of CRC cells after overexpression or knockdown of CYP1B1-AS1 respectively. The RNA binding protein NOP58 combined with CYP1B1-AS1 was verified by RIP and RNA Pull-down experiments. Functional recovery experiments validated the interaction between CYP1B1-AS1 and NOP58 in CRC cells. The changes of EMT-related proteins were detected by Western blot, and the half-life of transcription factor SNAIL mRNA were detected by RT-qPCR after overexpression or knockdown of NOP58.

Results

CYP1B1-AS1 was found to be significantly downregulated in CRC compared to adjacent noncancerous tissues. Experiments conducted in vitro and in vivo confirmed that upregulation of CYP1B1-AS1 significantly inhibited the proliferation, migration, and invasion of CRC cells. In addition, CYP1B1-AS1 can directly bind to NOP58 and negatively regulate NOP58. The effect of overexpression CYP1B1-AS1 was reversed by NOP58 overexpression. NOP58 regulates the EMT process of CRC cells by affecting the stability of EMT-related transcription factor SNAIL mRNA, and then affects the progress of CRC.

Conclusion

This research proves that CYP1B1-AS1 can inhibit the occurrence of EMT in CRC by binding with NOP58, thus delaying the progress of CRC. This finding indicates that CYP1B1-AS1 may be a novel biomarker to improve the diagnosis and treatment of CRC.

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Acknowledgment

The authors thank you sincerely to Jiangxi Key Laboratory of Molecular Medicine for offering excellent experimental facilities for this study.

Funding

This work was funded by Natural Science Foundation of Jiangxi Provincial [Grant No: 20171BAB205064] and National Natural Science Foundation of China [Grant No: 81860432].

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Authors and Affiliations

  1. Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, No.1 Minde Road, Nanchang, 330006, Jiangxi, China

    Zhao Wu, Fei Cheng, Lebin Yuan, Xiaodong Li, Zhigang Li, Zeyu Huang, Shengping Mao, Xing Chen, Bin Lai & Wei Shen

  2. The Second Clinical Medical College of Nanchang University, Nanchang, 330006, Jiangxi, China

    Zhao Wu, Lebin Yuan, Xiaodong Li, Zhigang Li, Zeyu Huang, Shengping Mao & Xing Chen

  3. Department of Ultrasound, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, China

    Jiwei Wang

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  1. Zhao Wu
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Contributions

Study conception and design performed by WS and BL; Developed the methodology, wrote, reviewed, and revised the manuscript performed by ZW; FC, LY, XL, ZL, ZH, SM, XC, JW contributed to data acquisition, analysis, and interpretation, as well as statistical analysis; Wei Shen provided technical and material support. All authors have read and approved the final version of the manuscript.

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Correspondence to Wei Shen.

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Wu, Z., Cheng, F., Yuan, L. et al. CYP1B1-AS1 Delays the Malignant Progression of Colorectal Cancer by Binding with NOP58. Dig Dis Sci 69, 437–452 (2024). https://doi.org/10.1007/s10620-023-08206-7

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  • DOI: https://doi.org/10.1007/s10620-023-08206-7

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