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KLF15 Inhibits Cell Proliferation in Gastric Cancer Cells via Up-Regulating CDKN1A/p21 and CDKN1C/p57 Expression

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Abstract

Background

Krüppel-like factors (KLFs) have been identified in multi-cancers and act as oncogenes or tumor suppressors. The function of KLF15, one member of KLFs, has not been well elucidated, especially in gastric cancer (GC).

Aims

This study was designed to investigate the prognostic value and biological functions of KLF15 in GC.

Methods

KLF15 protein expression in GC patients was evaluated by immunohistochemistry assays in 50 paired GC tissues and adjacent normal tissues, and correlations between KLF15 expression and clinicopathological characteristics and prognosis were analyzed. Then, we investigated the over-expression of KLF15 on cell proliferation and its mechanism in GC cells.

Results

KLF15 expression levels were significantly down-regulated in GC tissues compared to adjacent normal tissues. And KLF15 expression was negatively correlated with clinical stage, lymphatic metastasis, and distant metastasis. Furthermore, KLF15 expression could predict prognosis in patients with GC. Moreover, over-expression of KLF15 could inhibit cell proliferation partly via regulating CDKN1A/p21 and CDKN1C/p57.

Conclusion

These findings demonstrate that KLF15 plays a significant role in GC progression and could be a therapeutic target for GC.

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Acknowledgments

The authors are thankful to the National Natural Science Foundation of China for supporting this study (Grant Numbers 81672896, 81302012).

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    Authors and Affiliations

    1. Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, No.300 Guangzhou Road, Nanjing, People’s Republic of China

      Chongqi Sun, Pei Ma, Yutian Pan, Chenhui Zhao, Jie Liu, Wei Li & Yongqian Shu

    2. Department of Pathology, Yangzhou No.1 People’s Hospital, Yangzhou, People’s Republic of China

      Yanfen Wang

    3. Department of Pathology, Nanjing Medical University, Nanjing, People’s Republic of China

      Weitao Liu & Yingchen Qian

    4. Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China

      Qinnan Chen

    5. Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, Nanjing Medical University, Nanjing, People’s Republic of China

      Yongqian Shu

    6. Department of Medical Oncology, Affiliated Sir Run Run Hospital of Nanjing Medical University, Nanjing, People’s Republic of China

      Wei Li & Yongqian Shu

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    1. Chongqi Sun
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    Correspondence to Wei Li or Yongqian Shu.

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    Conflict of interest

    The authors declare that they have no conflict of interest.

    Ethical approval

    The study was performed according to the ethical standards of the institutional research committee and the standards set in the Declaration of Helsinki 1975. This article does not contain any studies with animals performed by any of the authors.

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    Informed consent was obtained from all individual participants included in the study.

    Additional information

    Yongqian Shu is the first corresponding author of this article.

    Chongqi Sun, Pei Ma, Yanfen Wang, and Weitao Liu contributed equally to this work and should be regarded as joint first authors.

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    Supplemental Figure 1

    Effects of KLF15 on KLF2 or KLF5 in GC cell lines. A. Real-time PCR analysis of KLF2 mRNA expression after over-expression of KLF15 in AGS and SGC-7901. Bars: SD. B. Real-time PCR analysis of KLF2 mRNA expression after over-expression of KLF15 in AGS and SGC-7901. Bars: SD (TIFF 2966 kb)

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    Cite this article

    Sun, C., Ma, P., Wang, Y. et al. KLF15 Inhibits Cell Proliferation in Gastric Cancer Cells via Up-Regulating CDKN1A/p21 and CDKN1C/p57 Expression. Dig Dis Sci 62, 1518–1526 (2017). https://doi.org/10.1007/s10620-017-4558-2

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