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Downregulation of Kruppel-like factor 2 is associated with poor prognosis for nonsmall-cell lung cancer

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Tumor Biology

Abstract

Kruppel-like factor 2 (KLF2) expression is diminished in many malignancies. However, its expression and role in nonsmall-cell lung cancer (NSCLC) remain unknown. In this study, we found that KLF2 levels were decreased in NSCLC tissues compared with adjacent normal tissues. Its expression level was significantly correlated with TNM stages, tumor size, and lymph node metastasis. Moreover, patients with low levels of KLF2 expression had a relatively poor prognosis. Furthermore, knockdown of KLF2 expression by siRNA could promote cell proliferation, while ectopic expression of KLF2 inhibited cell proliferation and promoted apoptosis in NSCLC cells partly via regulating CDKN1A/p21 and CDKN2B/p15 protein expression. Our findings present that decreased KLF2 could be identified as a poor prognostic biomarker in NSCLC and regulate cell proliferation and apoptosis.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (81172140, 81272532), Jiangsu Province Clinical Science and Technology projects (Clinical Research Center, BL2012008) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (Public Health and Preventive Medicine, JX10231801).

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None

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Authors and Affiliations

  1. Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, No. 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, China

    Li Yin, Tong-peng Xu, Wen-ming Chen & Yong-qian Shu

  2. Department of Respiratory Medicine, Huai’an First People’s Hospital, Nanjing Medical University, No. 6 Beijing Road West, Huai’an, Jiangsu, 223300, Jiangsu Province, China

    Ji-peng Wang

  3. Department of Medical Oncology, Huai’an First People’s Hospital, Nanjing Medical University, No. 6 Beijing Road West, Huai’an, Jiangsu, 223300, Jiangsu Province, China

    Ming-de Huang

  4. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, Jiangsu Province, China

    Rui Xia, Ming Sun & Er-bao Zhang

  5. Department of Gastrointestinal Surgery, Subei People’s Hospital of Jiangsu Province, Yangzhou University, Yangzhou, 225001, Jiangsu Province, China

    Xin-xin Liu & Rong Kong

Authors
  1. Li Yin
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  2. Ji-peng Wang
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  3. Tong-peng Xu
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  4. Wen-ming Chen
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  5. Ming-de Huang
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  6. Rui Xia
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  7. Xin-xin Liu
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  8. Rong Kong
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  9. Ming Sun
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  10. Er-bao Zhang
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  11. Yong-qian Shu
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Corresponding author

Correspondence to Yong-qian Shu.

Additional information

Li Yin, Ji-peng Wang, and Tong-peng Xu contributed equally to this work and should be regarded as joint first authors.

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Yin, L., Wang, Jp., Xu, Tp. et al. Downregulation of Kruppel-like factor 2 is associated with poor prognosis for nonsmall-cell lung cancer. Tumor Biol. 36, 3075–3084 (2015). https://doi.org/10.1007/s13277-014-2943-4

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  • DOI: https://doi.org/10.1007/s13277-014-2943-4

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