Abstract
We previously found that chicken egg white extract could promote cell survival and proliferation. In the present study, we further separated this extract into its components to identify those primarily responsible for promoting cell proliferation. Components of differing molecular weight were separated from chicken egg white extract by ultrafiltration and 293T cell cultures were supplemented with various concentrations. The effects on cell proliferation were subsequently determined by a CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit (Promega). We demonstrate that components from chicken egg white smaller than 3 kDa in size are able to function as active ingredients promoting cellular proliferation. This discovery may identify a new and convenient additive for cell culture media to promote cell growth and proliferation.
Similar content being viewed by others
References
Azuma N, Mori H, Kaminogawa S, Yamauchi K (1989) Presence of cell growth promoting peptides in chicken egg white extract. Agric Biol Chem 53:1291–1295
Besler M, Mine Y (1999) The major allergen from hen’s egg white: ovomucoid (Gal d 1). Internet Symp Food Allerg 1:137–146
Freberg CT, Dahl JA, Timoskainen S, Collas P (2007) Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract. Mol Biol Cell 18:1543–1553
Lee A, Molloy MP, Baker MS, Kapur A (2013) Tandem ion exchange fractionation of chicken egg white reveals the presence of proliferative bioactivity. J Agric Food Chem 61:4079–4088
Lingappa VR, Lingappa JR, Blobel G (1979) Chicken ovalbumin contains an internal signal sequence. Nature 281:117–121
McReynolds L, O’Malley BW, Nisbet AD, Fothergill JE, Givol D, Fields S, Robertson M, Brownlee GG (1978) Sequence of chicken ovalbumin mRNA. Nature 273:723–728
Mizunoya W, Tashima A, Sato Y, Tatsumi R, Ikeuchi Y (2015) The growth-promoting activity of egg white proteins in the C2C12 myoblast cell line. Anim Sci J 86:194–199
Ruan GP, Wang JX, Pang RQ, Yao X, Cai XM, Wang Q, Ma LH, Zhu XQ, Pan XH (2012) Treatment with chicken-egg-white or whole-egg extracts maintains and enhances the survival and differentiation of spleen cells. Cytotechnology 64:541–551
Taranger CK, Noer A, Sorensen AL, Hakelien AM, Boquest AC, Collas P (2005) Induction of dedifferentiation, genomewide transcriptional programming, and epigenetic reprogramming by extracts of carcinoma and embryonic stem cells. Mol Biol Cell 16:5719–5735
Acknowledgments
This work was supported by National Support Program (2014BAI01B01) and Yunnan Provincial Science and Technology Project (2013CA005). We thank American Journal Experts for assisting in the preparation of this manuscript.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Ruan, Gp., Yao, X., Wang, Jx. et al. Components of chicken egg white extract smaller than 3 kDa in size promote 293T cell proliferation. Cytotechnology 68, 1115–1122 (2016). https://doi.org/10.1007/s10616-015-9868-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10616-015-9868-7