Skip to main content
SpringerLink
Log in

Development and characterization of new microsatellite markers for ginseng (Panax ginseng C. A. Meyer)

  • Research Article
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

Panax ginseng C.A. Meyer, commonly known as Korean or Asian ginseng, is a perennial herb native to Korea and China. Its roots are highly prized for several medicinal properties. The present study describes development and characterization of twenty-two polymorphic microsatellite markers for this species. A total of 99 alleles were detected with an average of 4.5 alleles per locus across 20 accessions. Values for observed (H O ) and expected (H E ) heterozygosities ranged from 0.05 to 1.00 and from 0.18 to 0.73, respectively. Eleven loci deviated from Hardy–Weinberg equilibrium (P < 0.001). Significant (P < 0.05) heterozygote deficiency was observed at 13 loci. Exact test for linkage disequilibrium showed significant values (P < 0.05) between 12 pairs of loci. These microsatellite markers provide powerful tools for understanding population and conservation genetics of this species and also for genetic differentiation and authentication of different Panax species being used in commercial ginseng products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Dixit A, Jin MH, Chung JW, Yu JW, Chung HK, Ma KH, Park YJ, Cho EG (2005) Development of polymorphic microsatellite markers in sesame (Sesamum indicum L.). Mol Ecol Notes 5:736–738

    Article  CAS  Google Scholar 

  • Hon CC, Chow YC, Zeng FY, Leung FCC (2003) Genetic authentication of ginseng and other traditional Chinese medicine. Acta Pharmacologica Sinica 24:841–846

    PubMed  CAS  Google Scholar 

  • Kim KY (2004) Developing one step program (SSR Manager) for rapid identification of clones with SSRs and primer designing. Thesis, Seoul National University, Seoul, Republic of Korea

  • Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129

    Article  PubMed  CAS  Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    Article  PubMed  CAS  Google Scholar 

  • Yun T-K (2001) Brief introduction of Panax ginseng C.A. Meyer. J Korean Med Sci 16:3–5

    Google Scholar 

Download references

Acknowledgements

This study was supported by Bio-Green 21 program (Grant code-20050401034738) of Rural Development Administration (RDA) and ARPC (Agricultural R & D Promotion Center), Republic of Korea.

Author information

Authors and Affiliations

  1. Genetic Resources Division, National Institute of Agricultural Biotechnology, RDA, 225, Seodun-dong, Suwon, Kyunggi, 441-707, Republic of Korea

    Kyung-Ho Ma, Anupam Dixit, Dong-Yun Lee, Tae-San Kim, Eun-Gi Cho & Yong-Jin Park

  2. National Institute of Crop Science, RDA, Suwon, 441-707, Republic of Korea

    Young-Chang Kim

  3. Bioversity International-APO Regional Office, PO Box 236, UPM Post Office, Serdang, Selangor, Darul Ehsan, Malaysia

    Yong-Jin Park

Authors
  1. Kyung-Ho Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anupam Dixit
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Young-Chang Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dong-Yun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tae-San Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Eun-Gi Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yong-Jin Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yong-Jin Park.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ma, KH., Dixit, A., Kim, YC. et al. Development and characterization of new microsatellite markers for ginseng (Panax ginseng C. A. Meyer). Conserv Genet 8, 1507–1509 (2007). https://doi.org/10.1007/s10592-007-9284-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-007-9284-4

Keywords

Search

Navigation