Abstract
One of the pathological hallmarks of periventricular white matter injury is the vulnerability of pre-oligodendrocytes (preOLs) to hypoxia-ischemia (HI). There is increasing evidence that basic fibroblast growth factor (bFGF) is an important signaling molecule for neurogenesis and neuroprotection in the central nervous system. However, it is unknown whether bFGF protects preOLs from oxygen/glucose deprivation (OGD) damage in vitro and promotes remyelination in HI-induced rats. In this present study, bFGF exerted a protective effect on myelin by increasing the myelin thickness, the number of myelinated axons, and myelin basic protein expression in the HI-induced demyelinated neonatal rat corpus callosum. In vitro, bFGF ameliorated the impaired mitochondria and cell processes induced by OGD to promote the survival of isolated O4-positive preOLs. Additionally, the expression of fibroblast growth factor receptor 3 (FGFR3) was dramatically up-regulated in the preOLs after bFGF administration in vivo and in vitro. Thus, bFGF-stimulated remyelination in HI-induced rats by protecting the preOLs from hypoxic injury, and the mechanism involved may be mediated by FGFR3.
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Acknowledgments
All of these experiments were performed at Research Center for Neurobiology in Xuzhou Medical College. This study was supported by Grants from the National Natural Science Foundation of China (No. 81271345 to Ruiqin Yao, 81302519 to Xuebin Qu), the Natural Science Foundation of Jiangsu Province (No. BK20131132 to Ruiqin Yao, BK20130221 to Xuebin Qu), Qing Lan Project (Ruiqin Yao) of Jiangsu Province, the Xuzhou Project of Science and Technology for Social Development (No. XZZD1324 to Hongli Yu), and College Graduate Research and Innovation Project of Jiangsu Province (CXZZ13_0983 to Rui Guo).
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Xuebin Qu and Rui Guo have equally contributed to this article.
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Qu, X., Guo, R., Zhang, Z. et al. bFGF Protects Pre-oligodendrocytes from Oxygen/Glucose Deprivation Injury to Ameliorate Demyelination. Cell Mol Neurobiol 35, 913–920 (2015). https://doi.org/10.1007/s10571-015-0186-6
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DOI: https://doi.org/10.1007/s10571-015-0186-6