Skip to main content
Log in

Trametes trogii: A Biologic Powerful Tool for Dyes Decolorization and Detoxification

  • Published:
Catalysis Letters Aims and scope Submit manuscript

Abstract

The Trametes trogii laccase ability to decolorize azo and triarylmethane-dyes was studied in the absence of redox mediators. Various physico-chemical parameters were studied to achieve maximum dye decolorization. In crude-laccase presence, MG and BCG were completely degraded after 6 h at 50 mg/l of dye. Toxicity evaluation showed a final-product-detoxification.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Ben Younes S, Bouallagui Z, Gargoubi A, Sayadi S (2011) Investigation of dyes degradation intermediates with Scytalidium thermophilum laccase. Eur Food Res Technol 233:751–758

    Article  Google Scholar 

  2. Ben Younes S, Bouallagui Z, Sayadi S (2012) Catalytic behaviour and detoxifying ability of an atypical homotrimeric laccase from the thermophilic strain Scytalidium thermophilum on selected Azo and Triarylmethane dyes. J Mol Catal B Enzym 79:41–48

    Article  CAS  Google Scholar 

  3. Keharia H, Madamwar D (2003) Bioremediation concepts for treatment of dye containing wastewater: a review. Indian J Exp Biol 41:1068–1075

    CAS  Google Scholar 

  4. Daâssi D, Frikha F, Zouari-Mechichi H, Belbahri L, Woodward S, Mechichi T (2012) Application of response surface methodology to optimize decolourization of dyes by the laccase-mediator system. J Environ Manage 108:84–91

    Article  Google Scholar 

  5. Maalej-Kammoun M, Zouari-Mechichi H, Belbahri L, Woodward S, Mechichi T (2009) Malachite green decolourization and detoxification by the laccase from a newly isolated strain of Trametes sp. Int Biodeterior Biodegradation 63:600–606

    Article  CAS  Google Scholar 

  6. Junghanns C, Parra R, Keshavarz T, Schlosser D (2008) Towards higher laccase activities produced by aquatic ascomycetous fungi through combination of elicitors and an alternative substrate. Eng Life Sci 8:277–285

    Article  CAS  Google Scholar 

  7. Ben Younes S, Sayadi S (2012) Detoxification of Indigo carmine using a combined treatment via a novel trimeric thermostable laccase and microbial consortium. J Mol Catal B Enzym 87:62–68

    Article  Google Scholar 

  8. Hirschhäuser S, Fröhlich J (2007) Multiplex PCR for species discrimination of Sclerotiniaceae by novel laccase introns. Int J Food Microbiol 118:151–157

    Article  Google Scholar 

  9. Mechichi T, Mhiri N, Sayadi S (2006) Remazol Brilliant Blue R decolourization by the laccase from Trametes trogii. Chemosphere 64:998–1005

    Article  CAS  Google Scholar 

  10. Zouari-Mechichi H, Mechichi T, Dhouib A, Sayadi S, Martinez AT, Martinez MJ (2006) Laccase purification and characterization from Trametes trogii isolated in Tunisia: decolorization of textile dyes by the purified enzyme. Enzyme and Microbial Technology 39:141–148

    Article  CAS  Google Scholar 

  11. Chakroun H, Sayadi S, Mechichi T, Dhouib A (2009) High level of laccases production by Trametes trogii culture on olive mill wastewater-based media, application in textile dye decolorization. J Chem Technol Biotechnol 84:1527–1532

    Article  CAS  Google Scholar 

  12. Grassi E, Scodeller P, Filiel N, Carballo R, Levin L (2011) Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators. Int Biodeterior Biodegradation 65:635–643

    Article  CAS  Google Scholar 

  13. Garzillo AM, Colao MC, Buonocore V, Oliva R, Falcigno L, Saviano M, Santoro AM, Zappala R, Bonomo RP, Bianco C, Giardina P, Palmieri G, Sannia G (2001) Structural and kinetic characterization of native laccases from Pleurotus ostreatus, Rigidoporus lignosus and Trametes trogii. J Protein Chem 20:191–201

    Article  CAS  Google Scholar 

  14. Li K, Xu F, Eriksson KEL (1999) Comparison of fungal laccases and redox mediators in oxidation of a nonphenolic lignin model compound. Appl Environ Microbiol 65:2654–2660

    CAS  Google Scholar 

  15. Dhouib A, Hamza M, Zouari H, Mechichi T, Hmidi R, Labat M, Martinez MJ, Sayadi S (2005) Screening for ligninolytic enzyme production by diverse fungi from Tunisia. World J Microbiol Biotechnol 21:1514–1523

    Article  Google Scholar 

  16. Khlifi R, Belbahri L, Woodward S, Ellouz M, Dhouib A, Sayadi S, Mechichi T (2010) Decolourization and detoxification of textile industry wastewater by the laccase-mediator system. J Hazard Mater 175:802–808

    Article  CAS  Google Scholar 

  17. Ben Younes S, Sayadi S (2011) Purification and characterization of a novel trimeric and thermotolerant laccase produced from the ascomycete Scytalidium thermophilum strain. J Mol Catal B Enzym 73:35–42

    Article  CAS  Google Scholar 

  18. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  19. Ben Younes S, Mechichi T, Sayadi S (2007) Purification and characterization of the laccase secreted by the white rot fungus Perenniporia tephropora and its role in the decolourization of synthetic dyes. J Appl Microbiol 102:1033–1042

    CAS  Google Scholar 

  20. Capasso R, Evidenti A, Schivo L, Orru G, Marcialis MA, Cristinzio G (1995) Antibacterial polyphenols from olive oil mill waste waters. J Appl Bacteriol 79:393–398

    Article  CAS  Google Scholar 

  21. Levin L, Melignani E, Ramos AM (2010) Effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi. Dye decolorization by selected culture filtrates. Bioresour Technol 101:4554–4563

    Article  CAS  Google Scholar 

  22. Yan J, Niu J, Chen D, Chen Y, Irbis C (2014) Screening of Trametes strains for efficient decolorization of malachite green at high temperatures and ionic concentrations. Int Biodeterior Biodegradation 87:109–115

    Article  CAS  Google Scholar 

  23. Liu WX, Chao YP, Yang XQ, Bao HB, Qian SJ (2004) Biodecolorization of azo, anthraquinonic and triphenylmethane dyes by white-rot fungi and a laccase-secreting engineered strain. J Ind Microbiol Biotechnol 31:127–132

    Article  CAS  Google Scholar 

  24. Zhuo R, Ma L, Fan F, Gong Y, Wan X, Jiang M, Zhang X, Yang Y (2011) Decolorization of different dyes by a newly isolated white-rot fungi strain Ganoderma sp. En3 ands cloning and functional analysis of its laccase gene. J Hazard Mater 192:855–873

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Sonia Ben Younes or Sami Sayadi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ben Younes, S., Cherif, I., Dhouib, A. et al. Trametes trogii: A Biologic Powerful Tool for Dyes Decolorization and Detoxification. Catal Lett 146, 204–211 (2016). https://doi.org/10.1007/s10562-015-1629-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10562-015-1629-x

Keywords

Navigation