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Preparation and biocompatibility of demineralized bone matrix/sodium alginate putty

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Abstract

Demineralized bone matrix (DBM) powder is widely used for bone regeneration due to its osteoinductivity and osteoconductivity. However, difficulties with handling, tendency to migrate from graft sites and lack of stability after surgery sometimes limit the clinical utility of this material. In this work, the possibility of using sodium alginate (ALG) carrier to deliver DBM powder was assessed. DBM–ALG putty with the DBM:ALG weight ratio of 5:5, 6:4, 7:3, 8:2 were prepared, respectively. The properties of the formed composite, including discrete degree, washout property, pH, equilibrium swelling as well as cytotoxicity in vivo, were adopted to ascertain the optimal ratio of DBM and ALG. The discrete diameter increased from 1.25 cm (5:5) to 2.08 cm (8:2) with the increase of DBM content. There was significant difference between the 8:2 group and the other groups in discrete diameter. The ratio of DBM had a significant effect on the swelling value. The pH of composites showed an increase trend with the DBM ratio’s increase, when the ratio reached 7:3, the pH (7.22) was approximately equal to the body fluid. The proliferation of MC3T3-E1 cells was inhibited in the 5:5, 6:4 and 7:3 groups, while a slightly increased in the 8:2 group. The DBM–ALG with the optimal ratio of 7:3 was confirmed based on the results of the above mentioned. The histocompatibility of DBM–ALG (7:3) was examined using a rat model in which the materials were implanted subcutaneously, compared with the polyethylene, ALG and DBM. The study in vivo showed DBM–ALG (7:3) had a lower inflammatory response than DBM, a higher vascularization than ALG. The osteoinduction of DBM–ALG (7:3) was evaluated by co-culturing with MC3T3-E1 in vitro, compared with the DMEM, ALG and DBM. The results indicated calcification area in the DBM–ALG group was similar to that in the DBM group, larger than ALG group and DMEM group. The DBM–ALG (7:3) putty is promising as a directly injectable graft for repair of bone defect.

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References

  • Bae H, Zhao L, Zhu D, Kanim LE, Wang JC, Delamarter RB (2010) Variability across ten production lots of a single demineralized bone matrix product. J Bone Joint Surg Am 92(2):427–435

    Article  PubMed  Google Scholar 

  • D’Agostino P, Barbier O (2013) An investigation of the effect of AlloMatrix bone graft in distal radial fracture: a prospective randomised controlled clinical trial. Bone Joint J 95-B(11):1514–1520

    Article  PubMed  Google Scholar 

  • Decoster TA (2012) Low morbidity reported after iliac bone-graft harvesting. J Bone Joint Surg Am 94:e139

    Article  PubMed  Google Scholar 

  • Dowling MB, Chaturvedi A, MacIntire IC, Javvaji V, Gustin J, Raghavan SR, Scalea TM, Narayan M (2016) Determination of efficacy of a novel alginate dressing in a lethal arterial injury model in swine. Injury 47(10):2105–2109

    Article  PubMed  Google Scholar 

  • Eppley BL, Pietrzak WS, Blanton MW (2005) Allograft and alloplastic bone substitutes: a review of science and technology for the craniomaxillofacial surgeon. J Craniofac Surg 16(6):981–989

    Article  PubMed  Google Scholar 

  • Fan L, Ge H, Zou S, Xiao Y, Wen H, Li Y, Feng H, Nie M (2016) Sodium alginate conjugated graphene oxide as a new carrier for drug delivery system. Int J Biol Macromol 93(Pt A):582–590

    Article  CAS  PubMed  Google Scholar 

  • Gruskin E, Doll BA, Futrell FW, Schmitz JP, Hollinger JO (2012) Demineralized bone matrix in bone repair: history and use. Adv Drug Deliv Rev 64(12):1063–1077

    Article  CAS  PubMed  Google Scholar 

  • Han B, Tang B, Nimni ME (2003) Combined effects of phosphatidylcholine and demineralized bone matrix on bone induction. Connect Tissue Res 44:160–166

    Article  CAS  PubMed  Google Scholar 

  • Ivancic A, Macaev F, Aksakal F, Boldescu V, Pogrebnoi S, Duca G (2016) Preparation of alginate-chitosan-cyclodextrin micro- and nanoparticles loaded with anti-tuberculosis compounds. Beilstein J Nanotechnol 7:1208–1218

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jang CH, Park H, Cho YB, Song CH (2008) Mastoid obliteration using a hyaluronic acid gel to deliver a mesenchymal stem cells-loaded demineralized bone matrix: an experimental study. Int J Pediatr Otorhi 72:1627–1632

    Article  Google Scholar 

  • Jin HH, Kim DH, Kim TW, Shin KK, Jung JS, Park HC (2012) In vivo evaluation of porous hydroxyapatite/chitosan-alginate composite scaffolds for bone tissue engineering. Int J Biol Macromol 51:1079–1085

    Article  CAS  PubMed  Google Scholar 

  • Kolambkar YM, Dupont KM, Boerckel JD, Huebsch N, Mooney DJ, Hutmacher DW (2011) An alginate-based hybrid system for growth delivery in the functional repair of large bone defects. Biomaterials 32:65–74

    Article  CAS  PubMed  Google Scholar 

  • Lacey DC, Simmons PJ, Graves SE, Hamilton JA (2009) Proinflammatory cytokines inhibit osteogenic differentiation from stem cells: implications for bone repair during inflammation. Osteoarthr Cartil 17:735–742

    Article  CAS  PubMed  Google Scholar 

  • Lasa C, Hollinger J, Drohan W, Macphee M (1995) Delivery of demineralized bone powder by fibrin sealant. Plast Reconstr Surg 96:1409–1417

    Article  PubMed  Google Scholar 

  • Lee JH, Baek HR, Lee KM, Lee HK, Im SB, Kim YS, Lee JH, Chang BS, Lee CK (2014) The effect of poloxamer 407-based hydrogel on the osteoinductivity of demineralized bone matrix. Clin Orthop Surg 6(4):455–461

    Article  PubMed  PubMed Central  Google Scholar 

  • Li Z, Ramay HR, Hauch KD, Xiao D, Zhang M (2005) Chitosan alginate hybrid caffolds for bone tissue engineering. Biomaterials 26:3919–3928

    Article  CAS  PubMed  Google Scholar 

  • Li X, Jin L, Balian G, Laurencin CT, Anderson DG (2006) Demineralized bone matrixgelatin as scaffold for osteochondral tissue engineering. Biomaterials 27(11):2426–2433

    Article  CAS  PubMed  Google Scholar 

  • Moshaverinia A, Ansari S, Chen C, Xu X, Akiyama K, Snead ML (2013) Co-encapsulation of anti-BMP2 monoclonal antibody and mesenchymal stem cells in alginate microspheres for bone tissue engineering. Biomaterials 34:6572–6579

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nakaoka R, Hirano Y, Mooney DJ, Tsuchiya T, Matsuoka A (2013) Study on the potential of RGD- and PHSRN-modified alginates as artificial extracellular matrices for engineering bone. J Artif Organs 16(3):284–293

    Article  CAS  PubMed  Google Scholar 

  • Oakes DA, Lee CC, Lieberman JR (2003) An evaluation of human demineralized bone matrices in a rat femoral defect model. Clin Orthop Relat Res 413:281–290

    Article  Google Scholar 

  • Pietrzak WS, Woodell-May J, McDonald N (2006) Assay of bone morphogenetic protein-2, -4, and -7 in human demineralized bone matrix. J Craniofac Surg 17:84–90

    Article  PubMed  Google Scholar 

  • Reddi AH, Anderson WA (1976) Collagenous bone matrix-induced endochondral ossification hemopoiesis. J Cell Biol 69:557–572

    Article  CAS  PubMed  Google Scholar 

  • Rubert M, Alonso-Sande M, Monjo M, Ramis JM (2012) Evaluation of alginate and hyaluronic acid for their use in bone tissue engineering. Biointerphases 7:44

    Article  CAS  Google Scholar 

  • Sajesh KM, Jayakumar R, Nair SV, Chennazhi KP (2013) Biocompatible conducting chitosan/polypyrrole-alginate composite scaffold for bone tissue engineering. Int J Biol Macromol 62:465–471

    Article  CAS  PubMed  Google Scholar 

  • Sato T, Kikuchi M, Aizawa M (2017) Preparation of hydroxyapatite/collagen injectable bone paste with an anti-washout property utilizing sodium alginate. Part 1: influences of excess supplementation of calcium compounds. J Mater Sci Mater Med 28(3):49–55

    Article  PubMed  Google Scholar 

  • Schallenberger MA, Rossmeier K, Lovick HM, Meyer TR, Aberman HM, Juda GA (2014) Comparison of the osteogenic potential of OsteoSelect demineralized bone matrix putty to NovaBone calcium-phosphosilicate synthetic putty in a cranial defect model. Craniofac Surg 25(2):657–661

    Article  Google Scholar 

  • Schlag G, Redl H (1988) Fibrin sealant in orthopedic surgery. Clin Orthop 227:269–285

    CAS  PubMed  Google Scholar 

  • Sharawy M (1990) Bone induction in primates by demineralized bone matrix. J Oral Maxillofac Surg 48(5):547

    Article  CAS  PubMed  Google Scholar 

  • Urist MR (1965) Bone: formation by autoinduction. Science 150:893–899

    Article  CAS  PubMed  Google Scholar 

  • Wang JC, Alanay A, Mark D, Kanim LE, Campbell PA, Dawson EG, Lieberman JR (2007) A comparison of commercially available demineralized bone matrix for spinal fusion. Eur Spine J 16(8):1233–1240

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhang Y, Wang J, Wang J, Niu X, Liu J, Gao L, Zhai X, Chu K (2015) Preparation of porous PLA/DBM composite biomaterials and experimental research of repair rabbit radius segmental bone defect. Cell Tissue Bank 16(4):615–622

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by Natural Science Foundation of Shanxi Province of China (Award Nos. 201601D011126, 2006021050), Shanxi Scientific Rearch Fund of Traditional Chinese Medicine (Award No.2016ZYYC02), and Scientific Research Fund for the Doctoral Young Scholars, Shanxi University of Traditional Chinese Medicine (SXTCM) (Award No. 2014bk05).

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Authors and Affiliations

  1. Shanxi University of Traditional Chinese Medicine, Taiyuan, Shanxi, China

    Yumin Zhang, Yanmiao Ma, Xiaojun Niu, Jianchun Liu, Lan Gao, Xiaoyan Zhai, Kaibo Chu, Bo Han, Liwang Yang & Jue Wang

  2. Shanxi Medical University, Taiyuan, Shanxi, China

    Jianru Wang

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  1. Yumin Zhang
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  2. Jianru Wang
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  3. Yanmiao Ma
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Correspondence to Yumin Zhang.

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Zhang, Y., Wang, J., Ma, Y. et al. Preparation and biocompatibility of demineralized bone matrix/sodium alginate putty. Cell Tissue Bank 18, 205–216 (2017). https://doi.org/10.1007/s10561-017-9627-4

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  • DOI: https://doi.org/10.1007/s10561-017-9627-4

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