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Multi-parameter surface plasmon resonance instrument for multiple nucleic acid quantitative detection

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Abstract

Multiplex nucleic acid assays can simultaneously detect the characteristics of different target nucleic acids in complex mixtures and are used in disease diagnosis, environmental monitoring, and food safety. However, traditional nucleic acid amplification assays have limitations such as complicated operation, long detection time, unstable fluorescent labeling, and mutual interference of multiplex nucleic acids. We developed a real-time, rapid, and label-free surface plasmon resonance (SPR) instrument for multiplex nucleic acid detection. The multiparametric optical system based on total internal reflection solves the multiplex detection problem by cooperating with linear light source, prism, photodetector, and mechanical transmission system. An adaptive threshold consistency correction algorithm is proposed to solve the problem of inconsistent responsiveness of different detection channels and the inability of quantitative comparison. The instrument achieves label-free and amplification-free rapid detection of these biomarkers for miRNA-21 and miRNA-141, which are widely expressed in breast cancer and prostate cancer. The multiplex nucleic acid detection takes 30 min and the biosensor has good repeatability and specificity. The instrument has a limit of detection (LODs) of 50 nM for target oligonucleotides, and the smallest absolute amount of sample that can be detected is about 4 pmol. It provides a simple and efficient point-of-care testing (POCT) detection platform for small molecules such as DNA and miRNA.

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Data availability

The data presented in this study can be available by contacting the corresponding author, Prof. Dr. Lulu Zhang (llzhang@mail.buct.edu.cn).

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Acknowledgements

This work was supported in part by the National Natural Science Foundation of China under Grant 61971026, the Natural Science Foundation of Beijing Municipality under Grant 4202053, the National Natural Science Foundation of China under Grant 81871505, the Joint Project of BRC-BC (Biomedical Translational Engineering Research Center of BUCT-CJFH) under Grant XK2020-15, and the Fundamental Research Funds for the Central Universities under Grant BUCTRC201905.

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Authors and Affiliations

  1. College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Huixiang Wang, Honggang Wang, Yafeng Huang, Hao Zhang, Yongdong Fu, Xianbo Qiu, Duli Yu & Lulu Zhang

  2. Core Facility for Protein Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Zhenwei Yang & Yuanyuan Chen

Authors
  1. Huixiang Wang
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  2. Honggang Wang
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  3. Yafeng Huang
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  4. Hao Zhang
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  5. Yongdong Fu
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  6. Zhenwei Yang
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  7. Yuanyuan Chen
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  8. Xianbo Qiu
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  9. Duli Yu
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  10. Lulu Zhang
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Contributions

Huixiang Wang: Investigation, Conceptualization, Methodology, Validation, Writing – original draft. Honggang Wang: Investigation, Methodology. Hao Zhang: Investigation, Data curation. Yafeng Huang: Software. Yongdong Fu: Investigation, Data Processing. Zhenwei Yang: Investigation, Resources. Yuanyuan Chen: Formal analysis, Resources. Xianbo Qiu: Resources, Supervision. Duli Yu: Resources, Supervision. Lulu Zhang: Supervision, Conceptualization, Funding acquisition, Validation, Writing – review & editing

Corresponding author

Correspondence to Lulu Zhang.

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Wang, H., Wang, H., Huang, Y. et al. Multi-parameter surface plasmon resonance instrument for multiple nucleic acid quantitative detection. Biomed Microdevices 25, 24 (2023). https://doi.org/10.1007/s10544-023-00664-0

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  • DOI: https://doi.org/10.1007/s10544-023-00664-0

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