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Analysis of drug sensitivity of Escherichia Coli O157H7

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Abstract

Sensitive and rapid tests of Escherichia coli drug sensitivity is very important for health of human and animals. An E. coli immunosensor was built based on electrochemical detection and immune detection technologies, through pretreating screen-printed electrodes, and analyzing the optimal reaction concentration of antigen antibody binding with the AC impedance method. Based on the detection system combining the immunosensor and electrochemical workstation, tests were carried out to measure the accuracy of E. coli concentration and drug sensitivity, and error of the detection system was calibrated in accordance with data from the electrochemical workstation. E. coli O157:H7 can be detected in the range of 103 cfu/ml ~ 1012 cfu/ml, and the detection error controlled within 5%. Results from the electrochemical workstation and those from the detection device were consistent, and both demonstrated a greater inhibitory effect of antibiotics on E. coli than on Bacillus subtilis. The electrochemical detection system is highly efficient and accurate, and could be widely applied to E. coli drug sensitivity tests in clinical medicine.

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References

  • J.Y. Park, K. Park, G. Ok et al., Detection of Escherichia coli O157:H7 Using Automated Immunomagnetic Separation and Enzyme-Based Colorimetric Assay. Sensors 20, 1395 (2020)

    Article  Google Scholar 

  • Z. Liu, L. Zhao, Q. Zhang et al., Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field. Front. Microbiol. 10, 2644 (2019)

    Article  Google Scholar 

  • S.M. Saad, J. Abdullah, S.A. Rashid et al.,  A carbon dots based fluorescence sensing for the determination of Escherichia coli O157:H7. Measurement 107845 (2020)

  • S.H. Cho, Y.S. Lim, Y.H. Kang, Comparison of Antimicrobial Resistance in Escherichia coli Strains Isolated From Healthy Poultry and Swine Farm Workers Using Antibiotics in Korea. Osong Public Health Res. Perspect. 3, 151–155 (2012)

    Article  Google Scholar 

  • B. Wu, C. Qi, L. Wang et al., Detection of microbial aerosols in hospital wards and molecular identification and dissemination of drug resistance of Escherichia coli. Environ. Int. 137, 105479 (2020)

    Article  Google Scholar 

  • R. Maluta, B. Nicholson, C. Logue et al., Complete Genomic Sequence of an Avian Pathogenic Escherichia coli Strain of Serotype O7: HNT. Genome Announc. 4, e01611-15 (2016)

    Article  Google Scholar 

  • O. Reina, S. Tsutomu, O. Hiroshi et al., Pulmonary tuberculosis misdiagnosed as extensively drug-resistant tuberculosis due to selection of a nontuberculous mycobacterial colony from a preculture dish used for a drug-susceptibility test. Respir. Investig. 58, 216–219 (2020)

    Article  Google Scholar 

  • M. Mizoguchi, Y. Matsumoto, R. Saito et al., Direct antibiotic susceptibility testing of blood cultures of gram-negative bacilli using the Drug Susceptibility Testing Microfluidic (DSTM) device. J. Infect. Chemother. 26, 554–562 (2020)

    Article  Google Scholar 

  • A. Ayalew, R. Fikru, A. Dinka et al., Prevalence and antibiotic susceptibility pattern of Escherichia coli O157:H7 isolated from harvested fish at Lake Hayq and Tekeze dam. Northern Ethiopia Heliyon 5, e02996 (2019)

    Google Scholar 

  • J. Visvalingam, K. Palaniappan, R.A. Holley, In vitro enhancement of antibiotic susceptibility of drug resistant Escherichia coli by cinnamaldehyde. Food Control. 79, 288–291 (2017)

    Article  Google Scholar 

  • S. Arends, M. Canino, R. Mendes et al., Comparison of minimum inhibitory concentration results for gepotidacin obtained using agar dilution and broth microdilution methods. Diagn. Microbiol. Infect. Dis. 98, 115107 (2020)

    Article  Google Scholar 

  • G. Igawa, M. Cassey et al., Comparison of agar dilution and broth microdilution methods for Clostridium difficile antimicrobial susceptibility testing. J. Glob. Antimicrob. Resist. 7, 43–45 (2016)

    Article  Google Scholar 

  • E. Chryssanthou, M. Cuenca-Estrella, Comparison of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antibiotic Susceptibility Testing Proposed Standard and the E-Test with the NCCLS Broth Microdilution Method for Voriconazole and Caspofungin Susceptibility Testing of Yeast Species. J. Clin. Microbiol. 40, 3841–3844 (2002)

    Article  Google Scholar 

  • R. Huang, Z. Zhao, X. Ma et al., Targeting of tumor radioiodine therapy by expression of the sodium iodide symporter under control of the survivin promoter. Cancer Gene Ther. 18, 144–152 (2011)

    Article  Google Scholar 

  • G. Pfyffer, F. Palicova, S. Rusch-Gerdes, Testing of Susceptibility of Mycobacterium tuberculosis to Pyrazinamide with the Nonradiometric BACTEC MGIT 960 System. J. Clin. Microbiol. 40, 1670–1674 (2002)

    Article  Google Scholar 

  • P. Kohner, R. Patel, J. Uhl et al., Comparison of agar dilution, broth microdilution, E-test, disk diffusion, and automated Vitek methods for testing susceptibilities of Enterococcus spp. to vancomycin. J.Clin. Microbiol. 35, 3258–63 (1997)

    Article  Google Scholar 

  • P. Ruiz, F. Zerolo, M. Casal, Comparison of Susceptibility Testing of Mycobacterium tuberculosis Using the ESP Culture System II with That Using the BACTEC Method. J. Clin. Microbiol. 38, 4663–4664 (2000)

    Article  Google Scholar 

  • C. Wenisch, C. Moore, R. Krause et al., Antifungal susceptibility testing of fluconazole by cytometry correlations with clinical outcome. J. Clin. Microbiol. 39, 2458–2462 (2001)

    Article  Google Scholar 

  • P. Ekong, M. Sanderson, P. Shridhar et al., Bayesian estimation of sensitivity and specificity of culture- and PCR-based methods for the detection of six major non-O157 Escherichia coli serogroups in cattle feces. Prev. Vet. Med. 161, 90–99 (2018)

    Article  Google Scholar 

  • L. Noll, P. Shridhar, D. Dewsbury et al., A Comparison of Culture- and PCR-Based Methods to Detect Six Major Non-O157 Serogroups of Shiga Toxin-Producing Escherichia coli in Cattle Feces. PLoS One. 10, e0135446 (2015)

    Article  Google Scholar 

  • Z. Paddock, X. Shi, J. Bai et al., Applicability of a multiplex PCR to detect O26, O45, O103, O111, O121, O145, and O157 serogroups of Escherichia coli in cattle feces. Vet. Microbiol. 156, 381–388 (2012)

    Article  Google Scholar 

  • S. Sameh, F.I. Sonbol, et al., Molecular characterization of virulence and drug resistance genes-producing Escherichia coli isolated from chicken meat: Metal oxide nanoparticles as novel antibacterial agents. Microb. Pathog. 143, 104164 (2020)

    Article  Google Scholar 

  • S. Shahbazi, M. Reza et al., Distribution of Extended-Spectrum β-Lactam, Quinolone, and Carbapenem Resistance Genes, and Genetic Diversity among Uropathogenic Escherichia coli isolates in Tehran, Iran. J. Glob. Antimicrob. Resist. 14, 118–125 (2018)

    Article  Google Scholar 

  • S. Kurbanoglu, C. Erkmen, B. Uslu, Frontiers in electrochemical enzyme based biosensors for food and drug analysis. TrAC Trends Analyt. Chem. 124, 115809 (2020)

    Article  Google Scholar 

  • C. Yafei, Y. Yajie, W. Yu et al., Development of an Escherichia coli-based electrochemical biosensor for mycotoxin toxicity detection. Bioelectrochemistry 133, 107453 (2020)

    Article  Google Scholar 

  • Y. Lei, Application of Electrochemical Immuno-sensor Based on Ketamine Hydrochloride for the Detection of Sports Illicit Drugs. J. New Mater. Electrochem. Syst. 19, 139–143 (2016)

    Article  Google Scholar 

  • K. Theyagarajan, K. Dinakaran, A. Pangajam,  Highly sensitive electrochemical detection of E. coli O157:H7 using conductive carbon dot/ZnO nanorod/PANI composite electrode. Sens. Biosensing Res. 29, 100317 (2020)

  • J. Zhang, J. Wang et al., Rapid detection of Escherichia coli based on 16S rDNA nanogap network electrochemical biosensor. Biosens. Bioelectron. 118, 9–15 (2018)

    Article  Google Scholar 

Download references

Funding

The research is supported by the National Natural Science Foundation of China (Grant No. 31772651), Shanxi Foundation for Science and Technology Activities of Returned Students, Central government’s grant to the Shanxi province for local science and technology projects.

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Authors and Affiliations

  1. College of engineering, Shanxi Agricultural University, Jinzhong, 030800, People’s Republic of China

    Minzi Xu, Zheng Yang & Xiaobing Sun

  2. College of information science and engineering, Shanxi Agricultural University, Jinzhong, 030800, People’s Republic of China

    Zhenyu Liu, Runan Zhao, Huijin Zhao, Yaning Gu & Huifei Yang

  3. College of life sciences, Shanxi Agricultural University, Jinzhong, 030800, People’s Republic of China

    Yanbo Song

Authors
  1. Minzi Xu
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  2. Zhenyu Liu
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  3. Yanbo Song
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  4. Runan Zhao
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  5. Zheng Yang
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  6. Huijin Zhao
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  7. Xiaobing Sun
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  8. Yaning Gu
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  9. Huifei Yang
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Contributions

In this article, the author’s contributions are shown below: Methodology, Z.L.; writing—original draft preparation, M.X. and R.Z.; project administration, Z.L.; data curation, Z.Y., H.Z., Y.S., X.S., Y.G., and H.Y. helped to analyze the data. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Zhenyu Liu.

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Cite this article

Xu, M., Liu, Z., Song, Y. et al. Analysis of drug sensitivity of Escherichia Coli O157H7. Biomed Microdevices 23, 29 (2021). https://doi.org/10.1007/s10544-021-00565-0

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  • DOI: https://doi.org/10.1007/s10544-021-00565-0

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