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Molecular evaluation and antimicrobial susceptibility testing of Escherichia coli isolates from food products in Turkey

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Abstract

Some strains of Escherichia coli can be important food borne pathogens. Characterization and antimicrobial resistance testing of 28 E. coli isolates from random food samples obtained in Van, Turkey were performed. Primers for 6 indicator genes (fliC, stx1, stx2, eae, hlyA, and rfbE) for shiga toxin-producing E. coli and 5 indicator genes for each pathogroup (bfpA, aggR, ipaH, daaD, st, and lt) were used. E. coli isolates were also typed using pulsed field gel electrophoresis with the XbaI restriction enzyme. Antimicrobial susceptibility of E. coli isolates was determined using the disk diffusion method for 17 antimicrobials. E. coli isolates were non-pathogenic strains represented by 25 distinguishable PFGE patterns. Antimicrobial susceptibility testing revealed that more than 40% of the E. coli isolates showed resistance to ampicillin, sulphafurazole, and tetracycline. Antimicrobial susceptibility of commensal E. coli should be monitored because these bacteria are becoming reservoirs of antimicrobial resistance genes.

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Authors and Affiliations

  1. Department of Biotechnology, Middle East Technical University, Ankara, 106800, Turkey

    Emmanuel Owusu Kyere, Ece Bulut & Yeşim Soyer

  2. Department of Agricultural Biotechnology, Ahi Evran University, Kirşehir, 40100, Turkey

    M. Dilek Avşaroğlu

  3. Department of Food Engineering, Middle East Technical University, Ankara, 106800, Turkey

    Yeşim Soyer

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  1. Emmanuel Owusu Kyere
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  2. Ece Bulut
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  3. M. Dilek Avşaroğlu
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  4. Yeşim Soyer
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Correspondence to Yeşim Soyer.

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Kyere, E.O., Bulut, E., Dilek Avşaroğlu, M. et al. Molecular evaluation and antimicrobial susceptibility testing of Escherichia coli isolates from food products in Turkey. Food Sci Biotechnol 24, 1001–1009 (2015). https://doi.org/10.1007/s10068-015-0128-6

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  • DOI: https://doi.org/10.1007/s10068-015-0128-6

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