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Vertical flow paper-based plasmonic device for cysteine detection

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Abstract

Cystinuria, is an autosomal recessive genetic disorder involving increasingly high levels of poorly soluble cysteine in urine leading to formation of stones. Developing a facile, low-cost, point-of-care and selective sensor for diagnosis of cysteine is imperative. Accordingly, for the detection of cysteine, the present study demonstrates an inexpensive colorimetric, paper-based vertical flow plasmonic micro-well device with a two-minute turn-around time. The method encompasses the use of microbially-synthesized silver nanoparticles (AgNPs) that change from light brown / yellow to dark brown upon binding with Sulphur present in cysteine. This technique allows for visual detection up to 1 × 10−5 mM cysteine and can be easily offered as a rapid diagnostic test even at setups with minimal resources.

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References

  • V.V. Apyari, V.V. Arkhipova, A.I. Isachenko, P.A. Volkov, S.G. Dmitrienko, I.I. Torocheshnikova, Sensors Actuators B Chem. 260, 953–961 (2018)

    Article  Google Scholar 

  • M.J. Betts, R.B. Russell, Bioinformatics for geneticists (2003), pp. 289–316

    Book  Google Scholar 

  • C.S. Biyani, J.J. Cartledge, EAU-EBU update series 4, 175–183 (2006)

    Article  Google Scholar 

  • D.M. Cate, J.A. Adkins, J. Mettakoonpitak, C.S. Henry, Anal. Chem. 87, 19–41 (2015)

    Article  Google Scholar 

  • Z. Chen, S. Luo, C. Liu, Q. Cai, Anal. Bioanal. Chem. 395, 489–494 (2009)

    Article  Google Scholar 

  • T.A. Dankovich, Environ. Sci. Nano 1, 367–378 (2014)

    Article  Google Scholar 

  • P. Devaraj, P. Kumari, C. Aarti, A. Renganathan, J. Nanotechnol 2013, 598328 (2013) 5 pages

    Article  Google Scholar 

  • W. Dungchai, O. Chailapakul, C.S. Henry, Analyst 136, 77–82 (2011)

    Article  Google Scholar 

  • S. Fernandes, M. Mota, N. Lima, Nanosci. Nanotechnol. 5, 14–21 (2010)

    Google Scholar 

  • A. Guerra, M. Petrarulo, T. Schianchi, F. Allegri, T. Meschi, M. Bruno, A. Ramello, M. Baruffaldi, A. Novarini, L. Borghi, Lab. Med. 33, 214–217 (2002)

    Article  Google Scholar 

  • S. He, J. Yao, S. Xie, S. Pang, H. Gao, Chem. Phys. Lett. 343, 28–32 (2001)

    Article  Google Scholar 

  • S. Huang, Q. Xiao, R. Li, H.L. Guan, J. Liu, X.R. Liu, Z.K. He, Y. Liu, Anal. Chim. Acta 645, 73–78 (2009)

    Article  Google Scholar 

  • V.A. Kumar, T. Uchida, T. Mizuki, Y. Nakajima, Y. Katsube, T. Hanajiri, T. Maekawa, Adv. Nat. Sci. Nanosci. Nanotechnol. 7, 015002 (2016)

    Article  Google Scholar 

  • K. Kusmierek, R. Glowacki, E. Bald, Anal. Bioanal. Chem. 385, 855–860 (2006)

    Article  Google Scholar 

  • J.-S. Lee, P.A. Ulmann, M.S. Han, C.A. Mirkin, Nano Lett. 8, 529–533 (2008)

    Article  Google Scholar 

  • C.H. Lee, L. Tian, S. Singamaneni, ACS Appl. Mater. Interfaces 2, 3429–3435 (2010)

    Article  Google Scholar 

  • H. Li, Z. Cui, C. Han, Sensors Actuators B Chem. 143, 87–92 (2009)

    Article  Google Scholar 

  • L.-Q. Lu, Q. Gao, C. Song, X.-K. Tian, A.-W. Xu, RSC Adv. 4, 27297–27300 (2014)

    Article  Google Scholar 

  • L. Luo, X. Li, R.M. Crooks, Anal. Chem. 86, 12390–12397 (2014)

    Article  Google Scholar 

  • A.W. Martinez, S.T. Phillips, B.J. Wiley, M. Gupta, G.M. Whitesides, Lab Chip 8, 2146–2150 (2008)

    Article  Google Scholar 

  • S.M. Mehta, M.P. Sequeira, H. Muthurajana, J.S. D’Souza, Appl. Nanosci., 1–11 (2018)

  • N.M. Myers, E.N. Kernisan, M. Lieberman, Anal. Chem. 87, 3764–3770 (2015)

    Article  Google Scholar 

  • P. Prieto, V. Nistor, K. Nouneh, M. Oyama, M. Abd-Lefdil, R. Díaz, Appl. Surf. Sci. 258, 8807–8813 (2012)

    Article  Google Scholar 

  • A. Ravindran, S.P. Dhas, N. Chandrasekaran, A. Mukherjee, J. Exp. Nanosci. 8, 589–595 (2013)

    Article  Google Scholar 

  • O. Rusin, N.N.S. Luce, R.A. Agbaria, J.O. Escobedo, S. Jiang, I.M. Warner, F.B. Dawan, K. Lian, R.M. Strongin, J. Am. Chem. Soc. 126, 438–439 (2004)

    Article  Google Scholar 

  • N. Sumorok, D.S. Goldfarb, Curr. Opin. Nephrol. Hypertens. 22, 427–431 (2013)

    Article  Google Scholar 

  • O. Traxer, E. Lechevallier, C. Saussine, Prog. Urol. 18, 832–836 (2008)

    Article  Google Scholar 

  • P. Uznanski, J. Zakrzewska, F. Favier, S. Kazmierski, E. Bryszewska, J. Nanopart. Res. 19, 121 (2017)

    Article  Google Scholar 

  • A. Vasil’kov, R. Dovnar, S. Smotryn, N. Iaskevich, A. Naumkin, Antibiotics 7, 80 (2018)

    Article  Google Scholar 

  • J.E. Wear, B.G. Keevil, Clin. Chem. 51, 787–789 (2005)

    Article  Google Scholar 

  • R.F. Zeng, J.S. Lan, X.D. Li, H.F. Liang, Y. Liao, Y.J. Lu, T. Zhang, Y. Ding, Molecules, 22 (2017)

  • H. Zhang, W. Feng, G. Feng, Dyes Pigments 139, 73–78 (2017)

    Article  Google Scholar 

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Author notes

  1. Sourabh M. Mehta

    Present address: IITB-Monash Research Academy, Powai, Mumbai, 400076, India

Authors and Affiliations

  1. National Center for Nanosciences and Nanotechnology, University of Mumbai, Vidyanagari Campus, Kalina, Santacruz (East), Mumbai, Maharashtra, 400098, India

    Sourabh M. Mehta & Harries Muthurajan

  2. School of Biological Sciences, UM-DAE Centre for Excellence in Basic Sciences, University of Mumbai, Vidya Nagari, Kalina campus, Santacruz (East), Mumbai, 400098, India

    Shraddha Mehta & Jacinta S. D’Souza

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  1. Sourabh M. Mehta
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  2. Shraddha Mehta
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  3. Harries Muthurajan
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  4. Jacinta S. D’Souza
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Correspondence to Jacinta S. D’Souza.

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Mehta, S.M., Mehta, S., Muthurajan, H. et al. Vertical flow paper-based plasmonic device for cysteine detection. Biomed Microdevices 21, 55 (2019). https://doi.org/10.1007/s10544-019-0399-4

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