Abstract
Nucleic acids serve as biomarkers of disease and it is highly desirable to develop approaches to extract small number of such genomic extracts from human bodily fluids. Magnetic particles-based nucleic acid extraction is widely used for concentration of small amount of samples and is followed by DNA amplification in specific assays. However, approaches to integrate such magnetic particles based capture with micro and nanofluidic based assays are still lacking. In this report, we demonstrate a magnetophoretic-based approach for target-specific DNA extraction and concentration within a microfluidic device. This device features a large chamber for reducing flow velocity and an array of μ-magnets for enhancing magnetic flux density. With this strategy, the device is able to collect up to 95 % of the magnetic particles from the fluidic flow and to concentrate these magnetic particles in a collection region. Then an enzymatic reaction is used to detach the DNA from the magnetic particles within the microfluidic device, making the DNA available for subsequent analysis. Concentrations of over 1000-fold for 90 bp dsDNA molecules is demonstrated. This strategy can bridge the gap between detection of low concentration analytes from clinical samples and a range of micro and nanofluidic sensors and devices including nanopores, nano-cantilevers, and nanowires.
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Acknowledgments
The authors would like to acknowledge funding support from National Institute of Health (R21 CA155863), Oxford Nanopore Technologies U.K., and financial support from Mayo-Illinois Alliance for Technology Based Healthcare (http://mayoillinois.org/). Finally, authors would like to thank Dr. Gregory Damhorst for experimental advice on qPCR.
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Shim, S., Shim, J., Taylor, W.R. et al. Magnetophoretic-based microfluidic device for DNA Concentration. Biomed Microdevices 18, 28 (2016). https://doi.org/10.1007/s10544-016-0051-5
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DOI: https://doi.org/10.1007/s10544-016-0051-5