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Suppression of tomato SlGGP aggravates methyl viologen-mediated oxidative stress

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  • Published:
Biologia Plantarum

Abstract

Ascorbate (AsA) is an important antioxidant that can scavenge reactive oxygen species to protect plant cells against oxidative stress. Guanosine 5'-diphosphate (GDP)-L-galactose phosphorylase (GGP) is a key enzyme in the AsA biosynthetic pathway. To investigate the functions of GGP in AsA synthesis and oxidative stress tolerance in tomato, antisense lines with a reduced expression of SlGGP were obtained. Photobleaching after treatment of leaf disks with methyl viologen was more severe in transgenic lines compared to wild type (WT) plants. Moreover, compared with the WT plants, the transgenic plants showed a higher content of hydrogen peroxide, superoxide anion, malondialdehyde, as well as ion leakage, but a lower content of AsA and chlorophylls, ascorbate peroxidase activity, net photosynthetic rate, and maximal photochemical efficiency of photosystem II. Results of real-time quantitative polymerase chain reaction show that suppression of the SlGGP gene in the transgenic plants reduced their oxidative stress tolerance.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbate

CAT:

catalase

DHA:

dehydroascorbate

D-Man/L-Gal:

D-mannose/L-galactose

Fv/Fm :

variable to maximum chlorophyll fluorescence ratio (maximal photochemical efficiency of photosystem II)

GDP:

guanosine 5'-diphosphate

GGP:

GDP-L-galactose phosphorylase

MDA:

malondialdehyde

MV:

methyl viologen

O2 •− :

superoxide anion radical

PFD:

photon flux density

PN :

net photosynthetic rate

PS II:

photosystem II

REC:

relative electric conductance

ROS:

reactive oxygen species

SlGalLDH :

L-galactono-1,4-lactone dehydrogenase

SOD:

superoxide dismutase

WT:

wild type

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Authors and Affiliations

  1. College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China

    D.-Y. Yang, N.-N. Ma, Z.-M. Liu, X.-C. Ma, S.-J. Zhao & Q.-W. Meng

Authors
  1. D.-Y. Yang
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  2. N.-N. Ma
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  3. Z.-M. Liu
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  4. X.-C. Ma
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  5. S.-J. Zhao
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  6. Q.-W. Meng
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Corresponding authors

Correspondence to S.-J. Zhao or Q.-W. Meng.

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Acknowledgements: This research was supported by the State Key Basic Research and Development Plan of China (2015CB150105), the Natural Science Foundation of China (31171474, 31371553), and the Research Award Foundation for Outstanding Young Scientist of Shandong Province (2014BSB01031). The first two authors contributed equally to this work.

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Yang, DY., Ma, NN., Liu, ZM. et al. Suppression of tomato SlGGP aggravates methyl viologen-mediated oxidative stress. Biol Plant 60, 677–685 (2016). https://doi.org/10.1007/s10535-016-0628-0

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  • DOI: https://doi.org/10.1007/s10535-016-0628-0

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