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Cold-induced starch degradation in potato leaves — intercultivar differences in the gene expression and activity of key enzymes

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Exposure to low temperatures is one of the most important factors that generate abiotic stress in plants, and the rapid accumulation of soluble sugars belongs to significant metabolic responses to cold stress. The accumulation of soluble sugars may be at least partially triggered by an increased rate of starch degradation. The analysis of transcript profiles and starch degrading enzyme activities in leaves of two potato cultivars was performed during a 12-h exposure to 2 °C. An induction of β-amylase expression and activity as well as an accumulation of reducing sugars were observed in cv. Desiree. No accumulation of reducing sugars and no significant changes in the β-amylase activity were initially observed in cv. Russet Burbank. Surprisingly, an increased α-amylase activity was observed in the last hours of the experiment, which was accompanied by an increased amount of reducing sugars. The results indicate that the leaves of Desiree and Russet Burbank potatoes growing under cold stress may degrade starch via different pathways.

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Abbreviations

DPE1:

disproportionating enzyme 1

DPE2:

disproportionating enzyme 2

G1P:

glucose 1-phosphate

LDA:

limit dextrinase

SGH:

soluble heteroglycan

SPS:

sucrose-6-phosphate synthase

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  1. Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, PL-02776, Warsaw, Poland

    D. Sitnicka & S. Orzechowski

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  1. D. Sitnicka
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  2. S. Orzechowski
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Sitnicka, D., Orzechowski, S. Cold-induced starch degradation in potato leaves — intercultivar differences in the gene expression and activity of key enzymes. Biol Plant 58, 659–666 (2014). https://doi.org/10.1007/s10535-014-0453-2

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  • DOI: https://doi.org/10.1007/s10535-014-0453-2

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