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Black phosphorus-incorporated novel Ti-12Mo-10Zr implant for multimodal treatment of osteosarcoma

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Abstract

The repair and reconstruction of large bone defects after bone tumor resection is still a great clinical challenge. At present, orthopedic implant reconstruction is the mainstream treatment for repairing bone defects. However, according to clinical feedback, local tumor recurrence and nonunion of bone graft are common reasons leading to the failure of bone defect repair and reconstruction after bone tumor resection, which seriously threaten the physical and mental health of patients. On this basis, here the self-developed low modulus Ti-12Mo-10Zr alloy (TMZ) was chosen as substrate material. To improve its biological activity and osteointegration, calcium, oxygen, and phosphorus co-doped microporous coating was prepared on TMZ alloy by microarc oxidation (MAO). Then, black phosphorus (BP) nanosheets were incorporated onto MAO treated TMZ alloy to obtain multifunctional composites. The obtained BP-MAO-TMZ implant exhibited excellent photothermal effects and effective ablation of osteosarcoma cancer cells under the irradiation of 808 nm near infrared laser, while no photothermal or therapeutic effects were observed for TMZ alloy. Meanwhile, the structure/component bionic coating obtained after MAO treatment as well as the P-driven in situ biomineralization performance after incorporation of BP nanosheets endowed BP-MAO-TMZ implant with synergistic promoting effect on MC3T3-E1 osteoblasts’ activity, proliferation and differentiation ability. This study is expected to provide effective clinical solutions for problems of difficult bone regeneration and tumor recurrence after tumor resection in patients with bone tumors and to solve a series of medical problems such as poor prognosis and poor postoperative quality of patients life with malignant bone tumors.

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Acknowledgements

This work was supported by the Key Research and Development Program of Henan Province (222102310441) and the Henan Excellent Youth Science Foundation project (NO. 212300410039).

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Authors and Affiliations

  1. College of Medical Technology and Engineering, Henan University of Science and Technology, 263 Kaiyuan Avenue, Luoyang, 471023, China

    Bianyun Cai, Xueke Zhou, Xuan Zhou, Kun Lei, Meng Han, Zilin Zhang, Xiaofang Li & Guangda Li

  2. Department of Orthopedic Surgery and Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China

    Leizhen Huang

Authors
  1. Bianyun Cai
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  2. Leizhen Huang
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  3. Xueke Zhou
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  4. Xuan Zhou
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  5. Kun Lei
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  6. Meng Han
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  7. Zilin Zhang
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  8. Xiaofang Li
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  9. Guangda Li
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Contributions

BC and LH1 contributed equally to this work. Briefly, BC conceived ideas of this work and performed all the experimental work related to preparation and characterization of the material. LH carried out all the biological work. All authors participated in the data analysis and results discussion as well as the writing and revising of the manuscript. 

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Correspondence to Guangda Li.

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Bianyun Cai and Leizhen Huang contributed equally to this work.

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Cai, B., Huang, L., Zhou, X. et al. Black phosphorus-incorporated novel Ti-12Mo-10Zr implant for multimodal treatment of osteosarcoma. Biometals 37, 131–142 (2024). https://doi.org/10.1007/s10534-023-00533-6

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