Abstract
The mining and leakage of molybdenum (Mo) can cause environmental contamination which has not been realized until recently. Bacteria that can mitigate Mo-contamination was enriched and isolated. The low temperature and different pH conditions were considered to analysis its feasibility in Northern China which suffers from a long time of low temperatures every year. The result showed that the removal rate of MoO42− by Raoultella ornithinolytica A1 reached 30.46% at 25 °C and pH 7.0 in Luria–Bertani medium (LB). Meanwhile, A1 also showed some efficiency in the reduction of MoO42− in low phosphate molybdate medium (LPM), which reached optimum at the MoO42− concentration of 10 mM. The results of FTIR indicated that the cell wall performed an essential role in the MoO42− removal process, which was illustrated by the distribution of Mo in A1 (Mo bound to cell wall accounted for 92.29% of the total MoO42− removed). In addition, low temperature (10 °C) effect the removal rate of MoO42− by − 8.38 to 11.66%, indicating the potential for the in-situ microbial remediation of Mo-contaminated environments in low temperature areas.
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Acknowledgements
The authors thank Heilongjiang Provincial Research Academy of Environmental Sciences for financial support, and Harbin Institute of Technology for technical assistance in the laboratory.
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This work was financially supported by the Scientific Research Project of the Heilongjiang Provincial Institute of Scientific Research.
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Study design and organization were performed by JX. Experiment operation and sample analysis, writing-review and editing were performed by CL, WL, XZ and ZL. Conceptualization, formal analysis, writing-review and editing were performed by AL. All authors improved the manuscript through comments and text suggestions and all authors approved the final manuscript.
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Xing, J., Li, C., Li, W. et al. Isolation and identification of the molybdenum-resistant strain Raoultella ornithinolytica A1 and its effect on MoO42− in the environment. Biodegradation 34, 169–180 (2023). https://doi.org/10.1007/s10532-022-10011-4
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DOI: https://doi.org/10.1007/s10532-022-10011-4